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Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins.
EMBO J. 1997 May 01; 16(9):2535-44.EJ

Abstract

Rad51p is a eukaryotic homolog of RecA, the central homologous pairing and strand exchange protein in Escherichia coli. Rad54p belongs to the Swi2p/Snf2p family of DNA-stimulated ATPases. Both proteins are also important members of the RAD52 group which controls recombinational DNA damage repair of double-strand breaks and other DNA lesions in Saccharomyces cerevisiae. Here we demonstrate by genetic, molecular and biochemical criteria that Rad51 and Rad54 proteins interact. Strikingly, overexpression of Rad54p can functionally suppress the UV and methyl methanesulfonate sensitivity caused by a deletion of the RAD51 gene. However, no suppression was observed for the defects of rad51 cells in the repair of gamma-ray-induced DNA damage, mating type switching or spontaneous hetero-allelic recombination. This suppression is genetically dependent on the presence of two other members of the recombinational repair group, RAD55 and RAD57. Our data provide compelling evidence that Rad51 and Rad54 proteins interact in vivo and that this interaction is functionally important for recombinational DNA damage repair. As both proteins are conserved throughout evolution from yeasts to humans, a similar protein-protein interaction may be expected in other organisms.

Authors+Show Affiliations

Institute of General Microbiology, Bern, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9171366

Citation

Clever, B, et al. "Recombinational Repair in Yeast: Functional Interactions Between Rad51 and Rad54 Proteins." The EMBO Journal, vol. 16, no. 9, 1997, pp. 2535-44.
Clever B, Interthal H, Schmuckli-Maurer J, et al. Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. EMBO J. 1997;16(9):2535-44.
Clever, B., Interthal, H., Schmuckli-Maurer, J., King, J., Sigrist, M., & Heyer, W. D. (1997). Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. The EMBO Journal, 16(9), 2535-44.
Clever B, et al. Recombinational Repair in Yeast: Functional Interactions Between Rad51 and Rad54 Proteins. EMBO J. 1997 May 1;16(9):2535-44. PubMed PMID: 9171366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. AU - Clever,B, AU - Interthal,H, AU - Schmuckli-Maurer,J, AU - King,J, AU - Sigrist,M, AU - Heyer,W D, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 2535 EP - 44 JF - The EMBO journal JO - EMBO J VL - 16 IS - 9 N2 - Rad51p is a eukaryotic homolog of RecA, the central homologous pairing and strand exchange protein in Escherichia coli. Rad54p belongs to the Swi2p/Snf2p family of DNA-stimulated ATPases. Both proteins are also important members of the RAD52 group which controls recombinational DNA damage repair of double-strand breaks and other DNA lesions in Saccharomyces cerevisiae. Here we demonstrate by genetic, molecular and biochemical criteria that Rad51 and Rad54 proteins interact. Strikingly, overexpression of Rad54p can functionally suppress the UV and methyl methanesulfonate sensitivity caused by a deletion of the RAD51 gene. However, no suppression was observed for the defects of rad51 cells in the repair of gamma-ray-induced DNA damage, mating type switching or spontaneous hetero-allelic recombination. This suppression is genetically dependent on the presence of two other members of the recombinational repair group, RAD55 and RAD57. Our data provide compelling evidence that Rad51 and Rad54 proteins interact in vivo and that this interaction is functionally important for recombinational DNA damage repair. As both proteins are conserved throughout evolution from yeasts to humans, a similar protein-protein interaction may be expected in other organisms. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/9171366/Recombinational_repair_in_yeast:_functional_interactions_between_Rad51_and_Rad54_proteins_ L2 - https://doi.org/10.1093/emboj/16.9.2535 DB - PRIME DP - Unbound Medicine ER -