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NMR study of the galactomannans of Trichophyton mentagrophytes and Trichophyton rubrum.
Biochem J. 1997 Apr 01; 323 (Pt 1):297-305.BJ

Abstract

Around 90% of chronic dermatophyte infections are caused by the fungi Trichophyton mentagrophytes and Trichophyton rubrum. One of the causes of the chronic infection resides in the immunosuppressive effects of the cell-wall components of these organisms. Therefore we have attempted to identify the chemical structure of galactomannan, one of the major cell-wall components. The cell-wall polysaccharides secreted by T. mentagrophytes and T. rubrum were isolated from the culture medium and fractionated into three subfractions by DEAE-Sephadex chromatography. Analysis of each subfraction by NMR indicated that there are two kinds of polysaccharides present, i.e. mannan and galactomannan. The mannan has a linear backbone consisting of alpha1,6-linked mannose units, with alpha1,2-linked mannose units as side chains. The core mannan moiety of the galactomannan was analysed by a sequential NMR assignment method after removing the galactofuranose units by acid treatment. The result indicates that the mannan moiety has a linear repeating structure of alpha1,2-linked mannotetraose units connected by an alpha1,6 linkage. The H-1 signals of the two intermediary alpha1, 2-linked mannoses of the tetraose unit showed a significant upfield shift (Deltadelta=0.05-0.08 p.p.m.), due to the steric effect of an alpha1,6-linked mannose unit. The attachment point of the galactofuranose units was determined at C-3 of the core mannan by the assignment of the downfield-shifted 13C signals of the galactomannan compared with those of the acid-modified product. In these galactomannans there were no polygalactofuranosyl chains which have been found in Penicillium charlesii and Aspergillus fumigatus.

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Authors+Show Affiliations

Ikuta K
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981, Japan.
Shibata N
No affiliation info available
Blake JS
No affiliation info available
Dahl MV
No affiliation info available
Nelson RD
No affiliation info available
Hisamichi K
No affiliation info available
Kobayashi H
No affiliation info available
Suzuki S
No affiliation info available
Okawa Y
No affiliation info available

MeSH

Carbohydrate ConformationCarbohydrate SequenceMagnetic Resonance SpectroscopyMannansMolecular Sequence DataTrichophyton

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9173896

Citation

Ikuta, K, et al. "NMR Study of the Galactomannans of Trichophyton Mentagrophytes and Trichophyton Rubrum." The Biochemical Journal, vol. 323 (Pt 1), 1997, pp. 297-305.
Ikuta K, Shibata N, Blake JS, et al. NMR study of the galactomannans of Trichophyton mentagrophytes and Trichophyton rubrum. Biochem J. 1997;323 (Pt 1):297-305.
Ikuta, K., Shibata, N., Blake, J. S., Dahl, M. V., Nelson, R. D., Hisamichi, K., Kobayashi, H., Suzuki, S., & Okawa, Y. (1997). NMR study of the galactomannans of Trichophyton mentagrophytes and Trichophyton rubrum. The Biochemical Journal, 323 (Pt 1), 297-305.
Ikuta K, et al. NMR Study of the Galactomannans of Trichophyton Mentagrophytes and Trichophyton Rubrum. Biochem J. 1997 Apr 1;323 (Pt 1):297-305. PubMed PMID: 9173896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NMR study of the galactomannans of Trichophyton mentagrophytes and Trichophyton rubrum. AU - Ikuta,K, AU - Shibata,N, AU - Blake,J S, AU - Dahl,M V, AU - Nelson,R D, AU - Hisamichi,K, AU - Kobayashi,H, AU - Suzuki,S, AU - Okawa,Y, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 297 EP - 305 JF - The Biochemical journal JO - Biochem J VL - 323 (Pt 1) N2 - Around 90% of chronic dermatophyte infections are caused by the fungi Trichophyton mentagrophytes and Trichophyton rubrum. One of the causes of the chronic infection resides in the immunosuppressive effects of the cell-wall components of these organisms. Therefore we have attempted to identify the chemical structure of galactomannan, one of the major cell-wall components. The cell-wall polysaccharides secreted by T. mentagrophytes and T. rubrum were isolated from the culture medium and fractionated into three subfractions by DEAE-Sephadex chromatography. Analysis of each subfraction by NMR indicated that there are two kinds of polysaccharides present, i.e. mannan and galactomannan. The mannan has a linear backbone consisting of alpha1,6-linked mannose units, with alpha1,2-linked mannose units as side chains. The core mannan moiety of the galactomannan was analysed by a sequential NMR assignment method after removing the galactofuranose units by acid treatment. The result indicates that the mannan moiety has a linear repeating structure of alpha1,2-linked mannotetraose units connected by an alpha1,6 linkage. The H-1 signals of the two intermediary alpha1, 2-linked mannoses of the tetraose unit showed a significant upfield shift (Deltadelta=0.05-0.08 p.p.m.), due to the steric effect of an alpha1,6-linked mannose unit. The attachment point of the galactofuranose units was determined at C-3 of the core mannan by the assignment of the downfield-shifted 13C signals of the galactomannan compared with those of the acid-modified product. In these galactomannans there were no polygalactofuranosyl chains which have been found in Penicillium charlesii and Aspergillus fumigatus. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/9173896/NMR_study_of_the_galactomannans_of_Trichophyton_mentagrophytes_and_Trichophyton_rubrum_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj3230297 DB - PRIME DP - Unbound Medicine ER -