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Whole-blood immunoassay (SimpliRED) versus plasma immunoassay (NycoCard) for the diagnosis of clinically suspected deep vein thrombosis.
Vasa. 1997 May; 26(2):97-101.VASA

Abstract

BACKGROUND

The level of D-dimer in the blood reflects the level of lysed, cross-linked fibrin, and is a useful diagnostic marker in patients with clinically suspected deep vein thrombosis (DVT). In this study, two assays for the measurement of D-dimer levels were compared: the new, whole-blood immunoassay, SimpliRED, which can be performed at the patient's bedside in two minutes; and the plasma immunoassay, NycoCard.

PATIENTS AND METHODS

D-dimer levels were determined using these two techniques in 108 patients with clinically suspected DVT. To ascertain or rule out the diagnosis of DVT compression ultrasonography using a colour coded Duplex instrument was done. 8 doubtful cases were diagnosed by ascending phlebography. By these procedures DVT was confirmed in 33 patients and ruled out in the remaining 75 cases.

RESULTS

The SimpliRED assay exhibited a sensitivity of 1.0 (CI 0.89-1.0) and a specificity of 0.75 (CI 0.63-0.84); negative predictive value 1.0 (CI 0.94-1.0), positive predictive value 0.63 (CI 0.49-1.0). By contrast, the NycoCard assay displayed a sensitivity of 0.85 (CI 0.68-0.95) and a specificity of 0.65 (CI 0.53-0.76); negative predictive value 0.91 (CI 0.80-0.97), positive predictive value of 0.52 (CI 0.38-0.66).

CONCLUSION

The results show that the SimpliRED assay is a valuable tool in the diagnosis of clinically suspected DVT, especially when laboratory facilities are not accessible.

Authors+Show Affiliations

Mayer W
Department of Dermatology, Wilhelminenspital, Vienna.
Hirschwehr R
No affiliation info available
Hippmann G
No affiliation info available
Odpadlik H
No affiliation info available
Bayer P
No affiliation info available
Partsch H
No affiliation info available

MeSH

AdultAgedAged, 80 and overFemaleFibrin Fibrinogen Degradation ProductsHumansImmunoassayMaleMiddle AgedPlethysmographyPoint-of-Care SystemsPredictive Value of TestsThrombophlebitisUltrasonography, Doppler, Color

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9174385

Citation

Mayer, W, et al. "Whole-blood Immunoassay (SimpliRED) Versus Plasma Immunoassay (NycoCard) for the Diagnosis of Clinically Suspected Deep Vein Thrombosis." VASA. Zeitschrift Fur Gefasskrankheiten, vol. 26, no. 2, 1997, pp. 97-101.
Mayer W, Hirschwehr R, Hippmann G, et al. Whole-blood immunoassay (SimpliRED) versus plasma immunoassay (NycoCard) for the diagnosis of clinically suspected deep vein thrombosis. Vasa. 1997;26(2):97-101.
Mayer, W., Hirschwehr, R., Hippmann, G., Odpadlik, H., Bayer, P., & Partsch, H. (1997). Whole-blood immunoassay (SimpliRED) versus plasma immunoassay (NycoCard) for the diagnosis of clinically suspected deep vein thrombosis. VASA. Zeitschrift Fur Gefasskrankheiten, 26(2), 97-101.
Mayer W, et al. Whole-blood Immunoassay (SimpliRED) Versus Plasma Immunoassay (NycoCard) for the Diagnosis of Clinically Suspected Deep Vein Thrombosis. Vasa. 1997;26(2):97-101. PubMed PMID: 9174385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole-blood immunoassay (SimpliRED) versus plasma immunoassay (NycoCard) for the diagnosis of clinically suspected deep vein thrombosis. AU - Mayer,W, AU - Hirschwehr,R, AU - Hippmann,G, AU - Odpadlik,H, AU - Bayer,P, AU - Partsch,H, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 97 EP - 101 JF - VASA. Zeitschrift fur Gefasskrankheiten JO - Vasa VL - 26 IS - 2 N2 - BACKGROUND: The level of D-dimer in the blood reflects the level of lysed, cross-linked fibrin, and is a useful diagnostic marker in patients with clinically suspected deep vein thrombosis (DVT). In this study, two assays for the measurement of D-dimer levels were compared: the new, whole-blood immunoassay, SimpliRED, which can be performed at the patient's bedside in two minutes; and the plasma immunoassay, NycoCard. PATIENTS AND METHODS: D-dimer levels were determined using these two techniques in 108 patients with clinically suspected DVT. To ascertain or rule out the diagnosis of DVT compression ultrasonography using a colour coded Duplex instrument was done. 8 doubtful cases were diagnosed by ascending phlebography. By these procedures DVT was confirmed in 33 patients and ruled out in the remaining 75 cases. RESULTS: The SimpliRED assay exhibited a sensitivity of 1.0 (CI 0.89-1.0) and a specificity of 0.75 (CI 0.63-0.84); negative predictive value 1.0 (CI 0.94-1.0), positive predictive value 0.63 (CI 0.49-1.0). By contrast, the NycoCard assay displayed a sensitivity of 0.85 (CI 0.68-0.95) and a specificity of 0.65 (CI 0.53-0.76); negative predictive value 0.91 (CI 0.80-0.97), positive predictive value of 0.52 (CI 0.38-0.66). CONCLUSION: The results show that the SimpliRED assay is a valuable tool in the diagnosis of clinically suspected DVT, especially when laboratory facilities are not accessible. SN - 0301-1526 UR - https://www.unboundmedicine.com/medline/citation/9174385/Whole_blood_immunoassay__SimpliRED__versus_plasma_immunoassay__NycoCard__for_the_diagnosis_of_clinically_suspected_deep_vein_thrombosis_ L2 - http://www.diseaseinfosearch.org/result/7087 DB - PRIME DP - Unbound Medicine ER -