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Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin.
Eur J Immunol. 1997 May; 27(5):1236-41.EJ

Abstract

We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-alpha chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon. RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.

Authors+Show Affiliations

Allergy and Clinical Immunology, Imperial College School of Medicine at the National Heart & Lung Institute, London, GB.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9174616

Citation

Barata, L T., et al. "Allergen-induced Recruitment of Fc Epsilon RI+ Eosinophils in Human Atopic Skin." European Journal of Immunology, vol. 27, no. 5, 1997, pp. 1236-41.
Barata LT, Ying S, Grant JA, et al. Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin. Eur J Immunol. 1997;27(5):1236-41.
Barata, L. T., Ying, S., Grant, J. A., Humbert, M., Barkans, J., Meng, Q., Durham, S. R., & Kay, A. B. (1997). Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin. European Journal of Immunology, 27(5), 1236-41.
Barata LT, et al. Allergen-induced Recruitment of Fc Epsilon RI+ Eosinophils in Human Atopic Skin. Eur J Immunol. 1997;27(5):1236-41. PubMed PMID: 9174616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin. AU - Barata,L T, AU - Ying,S, AU - Grant,J A, AU - Humbert,M, AU - Barkans,J, AU - Meng,Q, AU - Durham,S R, AU - Kay,A B, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 1236 EP - 41 JF - European journal of immunology JO - Eur J Immunol VL - 27 IS - 5 N2 - We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-alpha chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon. RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/9174616/Allergen_induced_recruitment_of_Fc_epsilon_RI+_eosinophils_in_human_atopic_skin_ L2 - https://doi.org/10.1002/eji.1830270527 DB - PRIME DP - Unbound Medicine ER -