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In vitro toxicity testing of alcohol-soluble proteins from diploid wheat Triticum monococcum in celiac disease.
J Biochem Toxicol. 1996; 11(6):313-8.JB

Abstract

Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Trificum monococcum with contrasting storage protein compositions and bread-making characteristics were found unable to agglutinate K562(S) cells even at a peptide concentration as high as 14 g/L, agglutination being strongly correlated with toxicity in celiac disease. When fractionated by affinity chromatography on Sepharose-6B coupled with mannan, peptic-tryptic digests separated into three fractions. Fraction C peptides were shown to agglutinate K562(S) cells, whereas peptides in fractions A and B and in the mixed fraction B + C were inactive, suggesting that fraction B contains "protective" peptides that interfere with toxic peptides in fraction C in their agglutinating activity. These results offer an opportunity to study the biochemical and genetic bases of wheat toxicity at the diploid level. Moreover, the reduced toxicity, if any, of Triticum monococcum in the celiac disease, along with the good grain characteristics of some "monococcum" accessions, greatly increases the economical prospects of this wheat species.

Authors+Show Affiliations

Laboratorio di Metabolismo e Biochimica Patologica, Istituto Superiore di Sanità, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9176743

Citation

De Vincenzi, M, et al. "In Vitro Toxicity Testing of Alcohol-soluble Proteins From Diploid Wheat Triticum Monococcum in Celiac Disease." Journal of Biochemical Toxicology, vol. 11, no. 6, 1996, pp. 313-8.
De Vincenzi M, Luchetti R, Giovannini C, et al. In vitro toxicity testing of alcohol-soluble proteins from diploid wheat Triticum monococcum in celiac disease. J Biochem Toxicol. 1996;11(6):313-8.
De Vincenzi, M., Luchetti, R., Giovannini, C., Pogna, N. E., Saponaro, C., Galterio, G., & Gasbarrini, G. (1996). In vitro toxicity testing of alcohol-soluble proteins from diploid wheat Triticum monococcum in celiac disease. Journal of Biochemical Toxicology, 11(6), 313-8.
De Vincenzi M, et al. In Vitro Toxicity Testing of Alcohol-soluble Proteins From Diploid Wheat Triticum Monococcum in Celiac Disease. J Biochem Toxicol. 1996;11(6):313-8. PubMed PMID: 9176743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro toxicity testing of alcohol-soluble proteins from diploid wheat Triticum monococcum in celiac disease. AU - De Vincenzi,M, AU - Luchetti,R, AU - Giovannini,C, AU - Pogna,N E, AU - Saponaro,C, AU - Galterio,G, AU - Gasbarrini,G, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 313 EP - 8 JF - Journal of biochemical toxicology JO - J Biochem Toxicol VL - 11 IS - 6 N2 - Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Trificum monococcum with contrasting storage protein compositions and bread-making characteristics were found unable to agglutinate K562(S) cells even at a peptide concentration as high as 14 g/L, agglutination being strongly correlated with toxicity in celiac disease. When fractionated by affinity chromatography on Sepharose-6B coupled with mannan, peptic-tryptic digests separated into three fractions. Fraction C peptides were shown to agglutinate K562(S) cells, whereas peptides in fractions A and B and in the mixed fraction B + C were inactive, suggesting that fraction B contains "protective" peptides that interfere with toxic peptides in fraction C in their agglutinating activity. These results offer an opportunity to study the biochemical and genetic bases of wheat toxicity at the diploid level. Moreover, the reduced toxicity, if any, of Triticum monococcum in the celiac disease, along with the good grain characteristics of some "monococcum" accessions, greatly increases the economical prospects of this wheat species. SN - 0887-2082 UR - https://www.unboundmedicine.com/medline/citation/9176743/In_vitro_toxicity_testing_of_alcohol_soluble_proteins_from_diploid_wheat_Triticum_monococcum_in_celiac_disease_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0887-2082&date=1996&volume=11&issue=6&spage=313 DB - PRIME DP - Unbound Medicine ER -