Tags

Type your tag names separated by a space and hit enter

Peroxide-induced damage in lenses of transgenic mice with deficient and elevated levels of glutathione peroxidase.
Ophthalmologica. 1997; 211(3):192-200.O

Abstract

Transgenic mice with elevated glutathione peroxidase (GSHPx) activity and gene knockout animals with a deficiency of the enzyme were used to investigate the role of GSHPx in defending the lens against H2O2-induced damage. The effects of peroxide on cultured lenses were determined by using light and transmission electron microscopy to evaluate morphological changes occurring in the epithelium and superficial cortex of the central and equatorial regions of the lens. DNA single-strand breaks in the epithelium were also examined. Following a 30-min exposure to 25 microM H2O2, lenses from normal animals showed distinct changes in the morphology of both the epithelium and superficial cortex. The damage to these cells was extensive in lenses of gene knockout mice in which activity of GSHPx was undetectable. In marked contrast, lenses of transgenic mice, which had 5-fold higher activities of GSHPx, were able to resist the cytotoxic effects. Similar to damage to cell morphology, the extent of DNA strand breaks was significantly lower (40% of control) in H2O2-exposed lenses as compared to normal lenses while DNA damage in gene knockout lenses was 5 times greater than that of GSHPx-rich transgenic lenses. The present studies extend our previous findings on the role of the glutathione redox cycle in the detoxification of peroxide and demonstrate that an increase in GSHPx activity protects the lens against peroxide-induced changes in cell morphology and DNA strand breaks.

Authors+Show Affiliations

Eye Research Institute, Oakland University, Rochester, MI 48309-4401, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9176901

Citation

Reddy, V N., et al. "Peroxide-induced Damage in Lenses of Transgenic Mice With Deficient and Elevated Levels of Glutathione Peroxidase." Ophthalmologica. Journal International D'ophtalmologie. International Journal of Ophthalmology. Zeitschrift Fur Augenheilkunde, vol. 211, no. 3, 1997, pp. 192-200.
Reddy VN, Lin LR, Ho YS, et al. Peroxide-induced damage in lenses of transgenic mice with deficient and elevated levels of glutathione peroxidase. Ophthalmologica. 1997;211(3):192-200.
Reddy, V. N., Lin, L. R., Ho, Y. S., Magnenat, J. L., Ibaraki, N., Giblin, F. J., & Dang, L. (1997). Peroxide-induced damage in lenses of transgenic mice with deficient and elevated levels of glutathione peroxidase. Ophthalmologica. Journal International D'ophtalmologie. International Journal of Ophthalmology. Zeitschrift Fur Augenheilkunde, 211(3), 192-200.
Reddy VN, et al. Peroxide-induced Damage in Lenses of Transgenic Mice With Deficient and Elevated Levels of Glutathione Peroxidase. Ophthalmologica. 1997;211(3):192-200. PubMed PMID: 9176901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peroxide-induced damage in lenses of transgenic mice with deficient and elevated levels of glutathione peroxidase. AU - Reddy,V N, AU - Lin,L R, AU - Ho,Y S, AU - Magnenat,J L, AU - Ibaraki,N, AU - Giblin,F J, AU - Dang,L, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 192 EP - 200 JF - Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde JO - Ophthalmologica VL - 211 IS - 3 N2 - Transgenic mice with elevated glutathione peroxidase (GSHPx) activity and gene knockout animals with a deficiency of the enzyme were used to investigate the role of GSHPx in defending the lens against H2O2-induced damage. The effects of peroxide on cultured lenses were determined by using light and transmission electron microscopy to evaluate morphological changes occurring in the epithelium and superficial cortex of the central and equatorial regions of the lens. DNA single-strand breaks in the epithelium were also examined. Following a 30-min exposure to 25 microM H2O2, lenses from normal animals showed distinct changes in the morphology of both the epithelium and superficial cortex. The damage to these cells was extensive in lenses of gene knockout mice in which activity of GSHPx was undetectable. In marked contrast, lenses of transgenic mice, which had 5-fold higher activities of GSHPx, were able to resist the cytotoxic effects. Similar to damage to cell morphology, the extent of DNA strand breaks was significantly lower (40% of control) in H2O2-exposed lenses as compared to normal lenses while DNA damage in gene knockout lenses was 5 times greater than that of GSHPx-rich transgenic lenses. The present studies extend our previous findings on the role of the glutathione redox cycle in the detoxification of peroxide and demonstrate that an increase in GSHPx activity protects the lens against peroxide-induced changes in cell morphology and DNA strand breaks. SN - 0030-3755 UR - https://www.unboundmedicine.com/medline/citation/9176901/Peroxide_induced_damage_in_lenses_of_transgenic_mice_with_deficient_and_elevated_levels_of_glutathione_peroxidase_ L2 - https://www.karger.com?DOI=10.1159/000310788 DB - PRIME DP - Unbound Medicine ER -