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Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysis.
Anal Biochem. 1997 May 15; 248(1):7-14.AB

Abstract

In this study we report the development of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)-based methods for the structural characterization of muropeptides derived from peptidoglycan. Prior to analysis, peptidoglycan samples were subjected to enzymatic digestion with muramidase and the resulting muropeptides were purified by HPLC. A new matrix, 5-chloro-2-mercaptobenzothiazole, was employed for the MALDI-MS analysis. The results have demonstrated that sub-picomole to femtomole detection can be achieved in both positive mode and negative mode, allowing unambiguous determination of the molecular masses of monomeric and oligomeric muropeptides. Structural information from monomeric muropeptides was obtained by further postsource decay (PSD) analysis. Fragmentation patterns in positive mode and negative mode PSD were complementary for the elucidation of the peptide chain sequence. Lysostaphin digestion was also incorporated with MALDI mass mapping analysis for determination of peptide chain cross-linking patterns of muropeptide oligomers from Staphylococcus aureus strains.

Authors+Show Affiliations

Department of Biochemistry, Michigan State University, East Lansing 48824, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9177719

Citation

Xu, N, et al. "Structural Characterization of Peptidoglycan Muropeptides By Matrix-assisted Laser Desorption Ionization Mass Spectrometry and Postsource Decay Analysis." Analytical Biochemistry, vol. 248, no. 1, 1997, pp. 7-14.
Xu N, Huang ZH, de Jonge BL, et al. Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysis. Anal Biochem. 1997;248(1):7-14.
Xu, N., Huang, Z. H., de Jonge, B. L., & Gage, D. A. (1997). Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysis. Analytical Biochemistry, 248(1), 7-14.
Xu N, et al. Structural Characterization of Peptidoglycan Muropeptides By Matrix-assisted Laser Desorption Ionization Mass Spectrometry and Postsource Decay Analysis. Anal Biochem. 1997 May 15;248(1):7-14. PubMed PMID: 9177719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterization of peptidoglycan muropeptides by matrix-assisted laser desorption ionization mass spectrometry and postsource decay analysis. AU - Xu,N, AU - Huang,Z H, AU - de Jonge,B L, AU - Gage,D A, PY - 1997/5/15/pubmed PY - 1997/5/15/medline PY - 1997/5/15/entrez SP - 7 EP - 14 JF - Analytical biochemistry JO - Anal Biochem VL - 248 IS - 1 N2 - In this study we report the development of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)-based methods for the structural characterization of muropeptides derived from peptidoglycan. Prior to analysis, peptidoglycan samples were subjected to enzymatic digestion with muramidase and the resulting muropeptides were purified by HPLC. A new matrix, 5-chloro-2-mercaptobenzothiazole, was employed for the MALDI-MS analysis. The results have demonstrated that sub-picomole to femtomole detection can be achieved in both positive mode and negative mode, allowing unambiguous determination of the molecular masses of monomeric and oligomeric muropeptides. Structural information from monomeric muropeptides was obtained by further postsource decay (PSD) analysis. Fragmentation patterns in positive mode and negative mode PSD were complementary for the elucidation of the peptide chain sequence. Lysostaphin digestion was also incorporated with MALDI mass mapping analysis for determination of peptide chain cross-linking patterns of muropeptide oligomers from Staphylococcus aureus strains. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/9177719/Structural_characterization_of_peptidoglycan_muropeptides_by_matrix_assisted_laser_desorption_ionization_mass_spectrometry_and_postsource_decay_analysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(97)92073-X DB - PRIME DP - Unbound Medicine ER -