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Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics.
Anal Biochem. 1997 Jun 01; 248(2):277-87.AB

Abstract

The aim of the present study was to compare the performances of gas chromatography/mass spectrometry (GC/MS) and gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) in stable isotope kinetic studies. In the analysis of cholesterol and leucine, GC/C/IRMS gave precise and linear results over a large scale of 13C enrichment (-22 to +760 delta/1000 for cholesterol, -26 to +600 delta/1000 for leucine). Compared with GC/MS, GC/C/IRMS was much more accurate and reproducible, especially at low [13C]-cholesterol enrichment (-12 delta/1000), with cholesterol samples ranging from 0.11 to 17 ng. Cholesterol ester kinetics in rabbit plasma low-density lipoproteins was studied after injection of 3 mg [3,4-(13)C]cholesterol. A smooth and regular kinetic curve was obtained with GC/C/IRMS; results were much less reproducible with GC/MS. Finally, the performances of GC/C/IRMS were demonstrated in the simultaneous kinetic study of three human plasma apolipoproteins during a primed constant infusion of 0.7 mg.kg-1.h-1 L-[l-13C]leucine. Kinetic curves were obtained in very-low-density lipoproteins and low-density lipoproteins for apolipoprotein B100, and in high-density lipoproteins for apolipoproteins AI and AIL.

Authors+Show Affiliations

Laboratoire de Biochimie des Lipoprotéines, INSERM CJF 93-10, Faculté de Médecine, Dijon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9177755

Citation

Pont, F, et al. "Isotope Ratio Mass Spectrometry, Compared With Conventional Mass Spectrometry in Kinetic Studies at Low and High Enrichment Levels: Application to Lipoprotein Kinetics." Analytical Biochemistry, vol. 248, no. 2, 1997, pp. 277-87.
Pont F, Duvillard L, Maugeais C, et al. Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics. Anal Biochem. 1997;248(2):277-87.
Pont, F., Duvillard, L., Maugeais, C., Athias, A., Perségol, L., Gambert, P., & Vergès, B. (1997). Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics. Analytical Biochemistry, 248(2), 277-87.
Pont F, et al. Isotope Ratio Mass Spectrometry, Compared With Conventional Mass Spectrometry in Kinetic Studies at Low and High Enrichment Levels: Application to Lipoprotein Kinetics. Anal Biochem. 1997 Jun 1;248(2):277-87. PubMed PMID: 9177755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics. AU - Pont,F, AU - Duvillard,L, AU - Maugeais,C, AU - Athias,A, AU - Perségol,L, AU - Gambert,P, AU - Vergès,B, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 277 EP - 87 JF - Analytical biochemistry JO - Anal Biochem VL - 248 IS - 2 N2 - The aim of the present study was to compare the performances of gas chromatography/mass spectrometry (GC/MS) and gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) in stable isotope kinetic studies. In the analysis of cholesterol and leucine, GC/C/IRMS gave precise and linear results over a large scale of 13C enrichment (-22 to +760 delta/1000 for cholesterol, -26 to +600 delta/1000 for leucine). Compared with GC/MS, GC/C/IRMS was much more accurate and reproducible, especially at low [13C]-cholesterol enrichment (-12 delta/1000), with cholesterol samples ranging from 0.11 to 17 ng. Cholesterol ester kinetics in rabbit plasma low-density lipoproteins was studied after injection of 3 mg [3,4-(13)C]cholesterol. A smooth and regular kinetic curve was obtained with GC/C/IRMS; results were much less reproducible with GC/MS. Finally, the performances of GC/C/IRMS were demonstrated in the simultaneous kinetic study of three human plasma apolipoproteins during a primed constant infusion of 0.7 mg.kg-1.h-1 L-[l-13C]leucine. Kinetic curves were obtained in very-low-density lipoproteins and low-density lipoproteins for apolipoprotein B100, and in high-density lipoproteins for apolipoproteins AI and AIL. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/9177755/Isotope_ratio_mass_spectrometry_compared_with_conventional_mass_spectrometry_in_kinetic_studies_at_low_and_high_enrichment_levels:_application_to_lipoprotein_kinetics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(97)92130-8 DB - PRIME DP - Unbound Medicine ER -