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Biosafety guidelines for sorting of unfixed cells.
Cytometry. 1997 Jun 01; 28(2):99-117.C

Abstract

The International Society of Analytical Cytology (ISAC) Biohazard Working Group presents guidelines for sorting of unfixed cells, including known biohazardous samples, using jet-in-air, deflected-droplet cell sorters. There is a risk that personnel operating these instruments could become exposed to droplets and aerosols containing biological agents present in the samples. The following guidelines can aid in the prevention of exposures of laboratory personnel to pathogens contained in the sort samples. The document provides biosafety recommendations for sample handling, operator training and protection, laboratory facility design, and instrument setup and maintenance. In addition, it describes in detail methods for assessment of instrument aerosol containment. Recommendations provided here may also help laboratories to obtain institutional and/or regulatory agency approval for sorting of unfixed and known biohazardous samples.

Authors+Show Affiliations

Schmid I
Department of Hematology and Oncology, University of California at Los Angeles. schmid@cyto.medsch.ucla.edu
Nicholson JK
No affiliation info available
Giorgi JV
No affiliation info available
Janossy G
No affiliation info available
Kunkl A
No affiliation info available
Lopez PA
No affiliation info available
Perfetto S
No affiliation info available
Seamer LC
No affiliation info available
Dean PN
No affiliation info available

MeSH

Cell SeparationContainment of BiohazardsFlow CytometryGuidelines as TopicHumansSafety ManagementTissue Fixation

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9181299

Citation

Schmid, I, et al. "Biosafety Guidelines for Sorting of Unfixed Cells." Cytometry, vol. 28, no. 2, 1997, pp. 99-117.
Schmid I, Nicholson JK, Giorgi JV, et al. Biosafety guidelines for sorting of unfixed cells. Cytometry. 1997;28(2):99-117.
Schmid, I., Nicholson, J. K., Giorgi, J. V., Janossy, G., Kunkl, A., Lopez, P. A., Perfetto, S., Seamer, L. C., & Dean, P. N. (1997). Biosafety guidelines for sorting of unfixed cells. Cytometry, 28(2), 99-117.
Schmid I, et al. Biosafety Guidelines for Sorting of Unfixed Cells. Cytometry. 1997 Jun 1;28(2):99-117. PubMed PMID: 9181299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosafety guidelines for sorting of unfixed cells. AU - Schmid,I, AU - Nicholson,J K, AU - Giorgi,J V, AU - Janossy,G, AU - Kunkl,A, AU - Lopez,P A, AU - Perfetto,S, AU - Seamer,L C, AU - Dean,P N, PY - 1997/6/1/pubmed PY - 2000/6/20/medline PY - 1997/6/1/entrez SP - 99 EP - 117 JF - Cytometry JO - Cytometry VL - 28 IS - 2 N2 - The International Society of Analytical Cytology (ISAC) Biohazard Working Group presents guidelines for sorting of unfixed cells, including known biohazardous samples, using jet-in-air, deflected-droplet cell sorters. There is a risk that personnel operating these instruments could become exposed to droplets and aerosols containing biological agents present in the samples. The following guidelines can aid in the prevention of exposures of laboratory personnel to pathogens contained in the sort samples. The document provides biosafety recommendations for sample handling, operator training and protection, laboratory facility design, and instrument setup and maintenance. In addition, it describes in detail methods for assessment of instrument aerosol containment. Recommendations provided here may also help laboratories to obtain institutional and/or regulatory agency approval for sorting of unfixed and known biohazardous samples. SN - 0196-4763 UR - https://www.unboundmedicine.com/medline/citation/9181299/Biosafety_guidelines_for_sorting_of_unfixed_cells_ L2 - https://doi.org/10.1002/(sici)1097-0320(19970601)28:2<99::aid-cyto2>3.0.co;2-b DB - PRIME DP - Unbound Medicine ER -