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Diagnosis of scrub typhus in Malaysian aborigines using nested polymerase chain reaction.

Abstract

A rapid diagnostic system for scrub typhus using nested polymerase chain reaction (PCR) was applied to clinical samples from Malaysian Aborigines. Whole blood from twenty-four patients suspected of scrub typhus infection were tested using nested polymerase chain reaction and sera were evaluated by the indirect immunoperoxidase test. Antibody responses towards Rickettsia tsutsugamushi were observed in seventeen patients with the majority having high titers of IgG antibodies. Seven patients were seronegative. The nested PCR amplified R. tsutsugamushi DNA from six patients, of which two were negative serologically and four had high titers of IgG antibodies. Second samples collected seven days after treatment were negative by PCR testing. Nested PCR is highly sensitive and specific and may be used to provide rapid confirmation of scrub typhus cases in endemic region.

Authors+Show Affiliations

Bacteriology Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9185274

Citation

Tay, S T., et al. "Diagnosis of Scrub Typhus in Malaysian Aborigines Using Nested Polymerase Chain Reaction." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 27, no. 3, 1996, pp. 580-3.
Tay ST, Nazma S, Rohani MY. Diagnosis of scrub typhus in Malaysian aborigines using nested polymerase chain reaction. Southeast Asian J Trop Med Public Health. 1996;27(3):580-3.
Tay, S. T., Nazma, S., & Rohani, M. Y. (1996). Diagnosis of scrub typhus in Malaysian aborigines using nested polymerase chain reaction. The Southeast Asian Journal of Tropical Medicine and Public Health, 27(3), 580-3.
Tay ST, Nazma S, Rohani MY. Diagnosis of Scrub Typhus in Malaysian Aborigines Using Nested Polymerase Chain Reaction. Southeast Asian J Trop Med Public Health. 1996;27(3):580-3. PubMed PMID: 9185274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of scrub typhus in Malaysian aborigines using nested polymerase chain reaction. AU - Tay,S T, AU - Nazma,S, AU - Rohani,M Y, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - 580 EP - 3 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 27 IS - 3 N2 - A rapid diagnostic system for scrub typhus using nested polymerase chain reaction (PCR) was applied to clinical samples from Malaysian Aborigines. Whole blood from twenty-four patients suspected of scrub typhus infection were tested using nested polymerase chain reaction and sera were evaluated by the indirect immunoperoxidase test. Antibody responses towards Rickettsia tsutsugamushi were observed in seventeen patients with the majority having high titers of IgG antibodies. Seven patients were seronegative. The nested PCR amplified R. tsutsugamushi DNA from six patients, of which two were negative serologically and four had high titers of IgG antibodies. Second samples collected seven days after treatment were negative by PCR testing. Nested PCR is highly sensitive and specific and may be used to provide rapid confirmation of scrub typhus cases in endemic region. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/9185274/Diagnosis_of_scrub_typhus_in_Malaysian_aborigines_using_nested_polymerase_chain_reaction_ L2 - http://www.diseaseinfosearch.org/result/7277 DB - PRIME DP - Unbound Medicine ER -