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T7-promoter-based Escherichia coli expression system induced with bacteriophage M13HEP.
Chin J Biotechnol. 1996; 12(4):207-13.CJ

Abstract

The bacteriophage M13HEP was constructed by cloning the T7 RNA polymerase gene into phage M13mp18 RF DNA to express T7 RNA polymerase under the control of the lac promoter. Through M13HEP phage infection, T7 RNA polymerase could be introduced into an expression strain and heterologous genes under the control of the T7 promoter can be induced to express. Using this phage M13HEP induction system, many heterologous genes, especially some genes whose products are toxic to the host strain, were successfully expressed. By transferring F' pilli from E. coli XL1-blue to E. coli HMS174, a new E. coli strain HMS174F' was obtained to make the construction, expression, and single-stranded DNA rescue of the T7 expression plasmid be conveniently performed in the same strain.

Authors+Show Affiliations

Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9187491

Citation

Chen, C, et al. "T7-promoter-based Escherichia Coli Expression System Induced With Bacteriophage M13HEP." Chinese Journal of Biotechnology, vol. 12, no. 4, 1996, pp. 207-13.
Chen C, Huang H, Yang X, et al. T7-promoter-based Escherichia coli expression system induced with bacteriophage M13HEP. Chin J Biotechnol. 1996;12(4):207-13.
Chen, C., Huang, H., Yang, X., Xia, Q., Li, B., & Wang, Y. (1996). T7-promoter-based Escherichia coli expression system induced with bacteriophage M13HEP. Chinese Journal of Biotechnology, 12(4), 207-13.
Chen C, et al. T7-promoter-based Escherichia Coli Expression System Induced With Bacteriophage M13HEP. Chin J Biotechnol. 1996;12(4):207-13. PubMed PMID: 9187491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - T7-promoter-based Escherichia coli expression system induced with bacteriophage M13HEP. AU - Chen,C, AU - Huang,H, AU - Yang,X, AU - Xia,Q, AU - Li,B, AU - Wang,Y, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 207 EP - 13 JF - Chinese journal of biotechnology JO - Chin. J. Biotechnol. VL - 12 IS - 4 N2 - The bacteriophage M13HEP was constructed by cloning the T7 RNA polymerase gene into phage M13mp18 RF DNA to express T7 RNA polymerase under the control of the lac promoter. Through M13HEP phage infection, T7 RNA polymerase could be introduced into an expression strain and heterologous genes under the control of the T7 promoter can be induced to express. Using this phage M13HEP induction system, many heterologous genes, especially some genes whose products are toxic to the host strain, were successfully expressed. By transferring F' pilli from E. coli XL1-blue to E. coli HMS174, a new E. coli strain HMS174F' was obtained to make the construction, expression, and single-stranded DNA rescue of the T7 expression plasmid be conveniently performed in the same strain. SN - 1042-749X UR - https://www.unboundmedicine.com/medline/citation/9187491/T7_promoter_based_Escherichia_coli_expression_system_induced_with_bacteriophage_M13HEP_ DB - PRIME DP - Unbound Medicine ER -