TY - JOUR T1 - Determination of saccharides labelled with a fluorescent reagent, DBD-ProCZ, by liquid chromatography. AU - Toyo'oka,T, AU - Kuze,A, PY - 1997/5/1/pubmed PY - 2000/6/20/medline PY - 1997/5/1/entrez SP - 132 EP - 6 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 11 IS - 3 N2 - Saccharides were determined by high-performance liquid chromatography (HPLC) after precolumn tagging with 4-(2-carbazoylpyrrolidin-1-yl)-7-(N,N-dimethylaminosulphonyl)-2,1, 3-benzoxadiazole (DBD-ProCZ). The tagging conditions were optimized with D-glucose. Monosaccharides and oligosaccharides were efficiently labelled under conditions of 65 degrees C for 180 min in water: acetonitrile mixture containing 0.5% trichloroacetic acid (TCA). The resulting derivatives were detected at 540 nm (excitation at 450 nm) after separation by HPLC. The separation of individual saccharides was possible using water: acetonitrile as the mobile phase using a reversed-phase column (Inertsil ODS-80A) and a normal-phase column (TSKgel Amide-80). N-Acetyl-D-glucosamine (GluNAC), N-acetyllactosamine (LacNAC) and tri-N-acetyl-chitotriose (tri-N-AceChito), which are components of glycoconjugates, were completely separated with an isocratic elution using as Inertsil ODS column. The on-column detection limit with the proposed HPLC separation and fluorescence detection at the low pmol level. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/9192104/Determination_of_saccharides_labelled_with_a_fluorescent_reagent_DBD_ProCZ_by_liquid_chromatography_ L2 - https://doi.org/10.1002/(SICI)1099-0801(199705)11:3<132::AID-BMC633>3.0.CO;2-N DB - PRIME DP - Unbound Medicine ER -