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Bcl-2 does not protect Burkitt's lymphoma cells from oxidant-induced cell death.
Blood 1997; 89(12):4480-92Blood

Abstract

Bcl-2 is an oncogene that confers deregulated growth potential to B lymphocytes through its ability to inhibit apoptotic cell death. A specific molecular activity for the Bcl-2 protein has not been identified, but several lines of evidence have supported a role in protection of cells from oxidative stress. We investigated whether there is a correlation between expression of high levels of Bcl-2 and susceptibility of human Burkitt's lymphoma cell lines to H2O2-induced killing. The amount of H2O2 required to kill 50% of cells in 24 hours varied widely in the seven different lymphoma cell lines that were tested, ranging from 35 to 500 micromol/L H2O2. However, expression of high levels of endogenous Bcl-2 did not protect the cells from H2O2-induced killing, even though it was effective in protecting the cells from apoptosis induced by agents such as A23187. Thus, Bcl-2 was functional in preventing apoptosis but did not act in an antioxidant capacity. The results were confirmed using a Burkitt's lymphoma cell line overexpressing transfected bcl-2. The results may be explained by the observation that H2O2 was inefficient at inducing apoptosis in these mature B-cell lines. Nonapoptotic death induced by H2O2 was not prevented by Bcl-2.

Authors+Show Affiliations

Laboratory of Immunology, Food and Drug Administration, Center for Biologics Evaluation and Research, Bethesda, MD 20892-4555, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9192772

Citation

Lee, Y, and E Shacter. "Bcl-2 Does Not Protect Burkitt's Lymphoma Cells From Oxidant-induced Cell Death." Blood, vol. 89, no. 12, 1997, pp. 4480-92.
Lee Y, Shacter E. Bcl-2 does not protect Burkitt's lymphoma cells from oxidant-induced cell death. Blood. 1997;89(12):4480-92.
Lee, Y., & Shacter, E. (1997). Bcl-2 does not protect Burkitt's lymphoma cells from oxidant-induced cell death. Blood, 89(12), pp. 4480-92.
Lee Y, Shacter E. Bcl-2 Does Not Protect Burkitt's Lymphoma Cells From Oxidant-induced Cell Death. Blood. 1997 Jun 15;89(12):4480-92. PubMed PMID: 9192772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bcl-2 does not protect Burkitt's lymphoma cells from oxidant-induced cell death. AU - Lee,Y, AU - Shacter,E, PY - 1997/6/15/pubmed PY - 1997/6/15/medline PY - 1997/6/15/entrez SP - 4480 EP - 92 JF - Blood JO - Blood VL - 89 IS - 12 N2 - Bcl-2 is an oncogene that confers deregulated growth potential to B lymphocytes through its ability to inhibit apoptotic cell death. A specific molecular activity for the Bcl-2 protein has not been identified, but several lines of evidence have supported a role in protection of cells from oxidative stress. We investigated whether there is a correlation between expression of high levels of Bcl-2 and susceptibility of human Burkitt's lymphoma cell lines to H2O2-induced killing. The amount of H2O2 required to kill 50% of cells in 24 hours varied widely in the seven different lymphoma cell lines that were tested, ranging from 35 to 500 micromol/L H2O2. However, expression of high levels of endogenous Bcl-2 did not protect the cells from H2O2-induced killing, even though it was effective in protecting the cells from apoptosis induced by agents such as A23187. Thus, Bcl-2 was functional in preventing apoptosis but did not act in an antioxidant capacity. The results were confirmed using a Burkitt's lymphoma cell line overexpressing transfected bcl-2. The results may be explained by the observation that H2O2 was inefficient at inducing apoptosis in these mature B-cell lines. Nonapoptotic death induced by H2O2 was not prevented by Bcl-2. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/9192772/Bcl_2_does_not_protect_Burkitt's_lymphoma_cells_from_oxidant_induced_cell_death_ L2 - http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=9192772 DB - PRIME DP - Unbound Medicine ER -