Tags

Type your tag names separated by a space and hit enter

Distribution of fiber types determined by in situ hybridization of myosin heavy chain mRNA and enzyme histochemistry in rat skeletal muscles.
Cell Mol Biol (Noisy-le-grand). 1997 May; 43(3):319-27.CM

Abstract

We analyzed fiber types in rat skeletal muscles using a novel combination of in situ hybridization of myosin heavy chain (MyHC) mRNA, and enzyme histochemistry for succinate dehydrogenase (SD), which displayed metabolic properties. The fiber types were classified into the four major subtypes of I(beta/slow), IIA, IIX and IIB, and their intermediate types coexpressed two MyHC mRNAs: I and IIA, IIA and IIX, or IIX and IIB. The distribution of fiber types differed markedly in each skeletal muscle. The superficial region of limb muscles was composed mainly of fast-twitch fibers with oxidative-glycolytic and glycolytic activities, such as type IIX and type IIB. In contrast, the deep region was composed almost exclusively of type I and type IIA fibers both with oxidative activity. In this region, type IIA/IIX hybrid fibers were noted more frequently than type I/IIA and IIX/IIB hybrid fibers. In axial muscles, slow-twitch fibers and fast-twitch fibers composed predominantly of type IIB were distributed dispersively. The diaphragm and masseter showed a high proportion of type IIX and type IIB, respectively, to adapt to tissue-specific functional requirements and more frequently contained type IIX/IIB hybrid fibers than other observed muscles.

Authors+Show Affiliations

Department of Anatomy, Osaka Medical College, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9193786

Citation

Kanbara, K, et al. "Distribution of Fiber Types Determined By in Situ Hybridization of Myosin Heavy Chain mRNA and Enzyme Histochemistry in Rat Skeletal Muscles." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 43, no. 3, 1997, pp. 319-27.
Kanbara K, Sakai A, Watanabe M, et al. Distribution of fiber types determined by in situ hybridization of myosin heavy chain mRNA and enzyme histochemistry in rat skeletal muscles. Cell Mol Biol (Noisy-le-grand). 1997;43(3):319-27.
Kanbara, K., Sakai, A., Watanabe, M., Furuya, E., & Shimada, M. (1997). Distribution of fiber types determined by in situ hybridization of myosin heavy chain mRNA and enzyme histochemistry in rat skeletal muscles. Cellular and Molecular Biology (Noisy-le-Grand, France), 43(3), 319-27.
Kanbara K, et al. Distribution of Fiber Types Determined By in Situ Hybridization of Myosin Heavy Chain mRNA and Enzyme Histochemistry in Rat Skeletal Muscles. Cell Mol Biol (Noisy-le-grand). 1997;43(3):319-27. PubMed PMID: 9193786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of fiber types determined by in situ hybridization of myosin heavy chain mRNA and enzyme histochemistry in rat skeletal muscles. AU - Kanbara,K, AU - Sakai,A, AU - Watanabe,M, AU - Furuya,E, AU - Shimada,M, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 319 EP - 27 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 43 IS - 3 N2 - We analyzed fiber types in rat skeletal muscles using a novel combination of in situ hybridization of myosin heavy chain (MyHC) mRNA, and enzyme histochemistry for succinate dehydrogenase (SD), which displayed metabolic properties. The fiber types were classified into the four major subtypes of I(beta/slow), IIA, IIX and IIB, and their intermediate types coexpressed two MyHC mRNAs: I and IIA, IIA and IIX, or IIX and IIB. The distribution of fiber types differed markedly in each skeletal muscle. The superficial region of limb muscles was composed mainly of fast-twitch fibers with oxidative-glycolytic and glycolytic activities, such as type IIX and type IIB. In contrast, the deep region was composed almost exclusively of type I and type IIA fibers both with oxidative activity. In this region, type IIA/IIX hybrid fibers were noted more frequently than type I/IIA and IIX/IIB hybrid fibers. In axial muscles, slow-twitch fibers and fast-twitch fibers composed predominantly of type IIB were distributed dispersively. The diaphragm and masseter showed a high proportion of type IIX and type IIB, respectively, to adapt to tissue-specific functional requirements and more frequently contained type IIX/IIB hybrid fibers than other observed muscles. SN - 0145-5680 UR - https://www.unboundmedicine.com/medline/citation/9193786/Distribution_of_fiber_types_determined_by_in_situ_hybridization_of_myosin_heavy_chain_mRNA_and_enzyme_histochemistry_in_rat_skeletal_muscles_ DB - PRIME DP - Unbound Medicine ER -