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Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes.
Toxicol Appl Pharmacol. 1997 Jun; 144(2):279-86.TA

Abstract

Primary rat hepatocyte cultures exposed to tert-butylhydroperoxide (t-BHP) or cumene hydroperoxide were used to assess the antioxidative and protective potential of water-soluble extracts of artichoke leaves. Both hydroperoxides stimulated the production of malondialdehyde (MDA), particularly when the cells were pretreated with diethylmaleate (DEM) in order to diminish the level of cellular glutathione (GSH). Addition of artichoke extracts did not affect basal MDA production, but prevented the hydroperoxide-induced increase of MDA formation in a concentration-dependent manner when presented simultaneously or prior to the peroxides. The effective concentrations (down to 0.001 mg/ml) were well below the cytotoxic levels of the extracts which started above 1 mg/ml. The protective potential assessed by the LDH leakage assay and the MTT assay closely paralleled the reduction in MDA production and largely prevented hepatocyte necrosis induced by the hydroperoxides. The artichoke extracts did not affect the cellular level of glutathione (GSH), but diminished the loss of total GSH and the cellular leakage of GSSG resulting from exposure to t-BHP. Chlorogenic acid and cynarin accounted for only part of the antioxidative principle of the extracts which was resistant against tryptic digestion, boiling, acidification, and other treatments, but was slightly sensitive to alkalinization. These results demonstrate that artichoke extracts have a marked antioxidative and protective potential. Primary hepatocyte cultures seem suitable for identifying the constituents responsible for these effects and for elucidating their possible mode of action.

Authors+Show Affiliations

Physiologisch-Chemisches Institut, University of Tübingen, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9194411

Citation

Gebhardt, R. "Antioxidative and Protective Properties of Extracts From Leaves of the Artichoke (Cynara Scolymus L.) Against Hydroperoxide-induced Oxidative Stress in Cultured Rat Hepatocytes." Toxicology and Applied Pharmacology, vol. 144, no. 2, 1997, pp. 279-86.
Gebhardt R. Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicol Appl Pharmacol. 1997;144(2):279-86.
Gebhardt, R. (1997). Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicology and Applied Pharmacology, 144(2), 279-86.
Gebhardt R. Antioxidative and Protective Properties of Extracts From Leaves of the Artichoke (Cynara Scolymus L.) Against Hydroperoxide-induced Oxidative Stress in Cultured Rat Hepatocytes. Toxicol Appl Pharmacol. 1997;144(2):279-86. PubMed PMID: 9194411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. A1 - Gebhardt,R, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 279 EP - 86 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 144 IS - 2 N2 - Primary rat hepatocyte cultures exposed to tert-butylhydroperoxide (t-BHP) or cumene hydroperoxide were used to assess the antioxidative and protective potential of water-soluble extracts of artichoke leaves. Both hydroperoxides stimulated the production of malondialdehyde (MDA), particularly when the cells were pretreated with diethylmaleate (DEM) in order to diminish the level of cellular glutathione (GSH). Addition of artichoke extracts did not affect basal MDA production, but prevented the hydroperoxide-induced increase of MDA formation in a concentration-dependent manner when presented simultaneously or prior to the peroxides. The effective concentrations (down to 0.001 mg/ml) were well below the cytotoxic levels of the extracts which started above 1 mg/ml. The protective potential assessed by the LDH leakage assay and the MTT assay closely paralleled the reduction in MDA production and largely prevented hepatocyte necrosis induced by the hydroperoxides. The artichoke extracts did not affect the cellular level of glutathione (GSH), but diminished the loss of total GSH and the cellular leakage of GSSG resulting from exposure to t-BHP. Chlorogenic acid and cynarin accounted for only part of the antioxidative principle of the extracts which was resistant against tryptic digestion, boiling, acidification, and other treatments, but was slightly sensitive to alkalinization. These results demonstrate that artichoke extracts have a marked antioxidative and protective potential. Primary hepatocyte cultures seem suitable for identifying the constituents responsible for these effects and for elucidating their possible mode of action. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/9194411/Antioxidative_and_protective_properties_of_extracts_from_leaves_of_the_artichoke__Cynara_scolymus_L___against_hydroperoxide_induced_oxidative_stress_in_cultured_rat_hepatocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(97)98130-8 DB - PRIME DP - Unbound Medicine ER -