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Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock.
Mol Microbiol 1997; 24(4):687-95MM

Abstract

Synthesis of trehalose in the yeast Saccharomyces cerevisiae is catalysed by the trehalose-6-phosphate (Tre6P) synthase/phosphatase complex, which is composed of at least three different subunits encoded by the genes TPS1, TPS2, and TSL1. Previous studies indicated that Tps1 and Tps2 carry the catalytic activities of trehalose synthesis, namely Tre6P synthase (Tps1) and Tre6P phosphatase (Tps2), while TsI1 was suggested to have regulatory functions. In this study two different approaches have been used to clarify the molecular composition of the trehalose synthase complex as well as the functional role of its potential subunits. Two-hybrid analyses of the in vivo interactions of Tps1, Tps2, TsI1, and Tps3, a protein with high homology to TsI1, revealed that both TsI1 and Tps3 can interact with Tps1 and Tps2; the latter two proteins also interact with each other. In addition, trehalose metabolism upon heat shock was analysed in a set of 16 isogenic yeast strains carrying deletions of TPS1, TPS2, TSL1, and TPS3 in all possible combinations. These results not only confirm the previously suggested roles for Tps1 and Tps2, but also provide, for the first time, evidence that TsI1 and Tps3 may share a common function with respect to regulation and/or structural stabilization of the Tre6P synthase/phosphatase complex in exponentially growing, heat-shocked cells.

Authors+Show Affiliations

Botanisches Institut der Universität, Basel, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9194697

Citation

Reinders, A, et al. "Structural Analysis of the Subunits of the Trehalose-6-phosphate Synthase/phosphatase Complex in Saccharomyces Cerevisiae and Their Function During Heat Shock." Molecular Microbiology, vol. 24, no. 4, 1997, pp. 687-95.
Reinders A, Bürckert N, Hohmann S, et al. Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. Mol Microbiol. 1997;24(4):687-95.
Reinders, A., Bürckert, N., Hohmann, S., Thevelein, J. M., Boller, T., Wiemken, A., & De Virgilio, C. (1997). Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. Molecular Microbiology, 24(4), pp. 687-95.
Reinders A, et al. Structural Analysis of the Subunits of the Trehalose-6-phosphate Synthase/phosphatase Complex in Saccharomyces Cerevisiae and Their Function During Heat Shock. Mol Microbiol. 1997;24(4):687-95. PubMed PMID: 9194697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. AU - Reinders,A, AU - Bürckert,N, AU - Hohmann,S, AU - Thevelein,J M, AU - Boller,T, AU - Wiemken,A, AU - De Virgilio,C, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 687 EP - 95 JF - Molecular microbiology JO - Mol. Microbiol. VL - 24 IS - 4 N2 - Synthesis of trehalose in the yeast Saccharomyces cerevisiae is catalysed by the trehalose-6-phosphate (Tre6P) synthase/phosphatase complex, which is composed of at least three different subunits encoded by the genes TPS1, TPS2, and TSL1. Previous studies indicated that Tps1 and Tps2 carry the catalytic activities of trehalose synthesis, namely Tre6P synthase (Tps1) and Tre6P phosphatase (Tps2), while TsI1 was suggested to have regulatory functions. In this study two different approaches have been used to clarify the molecular composition of the trehalose synthase complex as well as the functional role of its potential subunits. Two-hybrid analyses of the in vivo interactions of Tps1, Tps2, TsI1, and Tps3, a protein with high homology to TsI1, revealed that both TsI1 and Tps3 can interact with Tps1 and Tps2; the latter two proteins also interact with each other. In addition, trehalose metabolism upon heat shock was analysed in a set of 16 isogenic yeast strains carrying deletions of TPS1, TPS2, TSL1, and TPS3 in all possible combinations. These results not only confirm the previously suggested roles for Tps1 and Tps2, but also provide, for the first time, evidence that TsI1 and Tps3 may share a common function with respect to regulation and/or structural stabilization of the Tre6P synthase/phosphatase complex in exponentially growing, heat-shocked cells. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/9194697/Structural_analysis_of_the_subunits_of_the_trehalose_6_phosphate_synthase/phosphatase_complex_in_Saccharomyces_cerevisiae_and_their_function_during_heat_shock_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1997&volume=24&issue=4&spage=687 DB - PRIME DP - Unbound Medicine ER -