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Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches.
Mol Microbiol. 1997 May; 24(4):697-709.MM

Abstract

Salmonella typhimurium initiates infection of a host by invading M cells of Peyer's patches within the small intestine. The ability of the bacteria to invade mammalian cells has been shown to be regulated by environmental conditions, including oxygen concentrations, osmolarity, and growth phase. We have previously created oxygen-regulated Tn5lacZY S. typhimurium mutants that are defective in invasion. We have now identified the invasion genes disrupted by eight of the transposon insertions. These genes encode transcriptional regulators (hilA and invF), type III secretory components (orgA, invG and spaR) and secreted proteins (invC and invD). Examination of the protein-secretion profiles of the non-invasive mutants indicated that each of the mutants was defective in secretion of between one and six proteins. We have also demonstrated that the loss of tissue culture cell invasiveness corresponds to an inability to invade and destroy M cells of Peyer's patches in a murine ligated loop model. Virulence studies, performed in mice, demonstrated that these defects significantly reduced the ability of the mutants to cause murine typhoid fever by an oral route of infection. Virulence by an intraperitoneal route of infection was unaffected. The data indicate that in vitro invasiveness, invasion-protein secretion, and M-cell invasion are critical indicators of S. typhimurium virulence.

Authors+Show Affiliations

Department of Microbiology, University of Iowa School of Medicine, Iowa City 52242, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9194698

Citation

Penheiter, K L., et al. "Non-invasive Salmonella Typhimurium Mutants Are Avirulent Because of an Inability to Enter and Destroy M Cells of Ileal Peyer's Patches." Molecular Microbiology, vol. 24, no. 4, 1997, pp. 697-709.
Penheiter KL, Mathur N, Giles D, et al. Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches. Mol Microbiol. 1997;24(4):697-709.
Penheiter, K. L., Mathur, N., Giles, D., Fahlen, T., & Jones, B. D. (1997). Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches. Molecular Microbiology, 24(4), 697-709.
Penheiter KL, et al. Non-invasive Salmonella Typhimurium Mutants Are Avirulent Because of an Inability to Enter and Destroy M Cells of Ileal Peyer's Patches. Mol Microbiol. 1997;24(4):697-709. PubMed PMID: 9194698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches. AU - Penheiter,K L, AU - Mathur,N, AU - Giles,D, AU - Fahlen,T, AU - Jones,B D, PY - 1997/5/1/pubmed PY - 1997/5/1/medline PY - 1997/5/1/entrez SP - 697 EP - 709 JF - Molecular microbiology JO - Mol Microbiol VL - 24 IS - 4 N2 - Salmonella typhimurium initiates infection of a host by invading M cells of Peyer's patches within the small intestine. The ability of the bacteria to invade mammalian cells has been shown to be regulated by environmental conditions, including oxygen concentrations, osmolarity, and growth phase. We have previously created oxygen-regulated Tn5lacZY S. typhimurium mutants that are defective in invasion. We have now identified the invasion genes disrupted by eight of the transposon insertions. These genes encode transcriptional regulators (hilA and invF), type III secretory components (orgA, invG and spaR) and secreted proteins (invC and invD). Examination of the protein-secretion profiles of the non-invasive mutants indicated that each of the mutants was defective in secretion of between one and six proteins. We have also demonstrated that the loss of tissue culture cell invasiveness corresponds to an inability to invade and destroy M cells of Peyer's patches in a murine ligated loop model. Virulence studies, performed in mice, demonstrated that these defects significantly reduced the ability of the mutants to cause murine typhoid fever by an oral route of infection. Virulence by an intraperitoneal route of infection was unaffected. The data indicate that in vitro invasiveness, invasion-protein secretion, and M-cell invasion are critical indicators of S. typhimurium virulence. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/9194698/Non_invasive_Salmonella_typhimurium_mutants_are_avirulent_because_of_an_inability_to_enter_and_destroy_M_cells_of_ileal_Peyer's_patches_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1997&volume=24&issue=4&spage=697 DB - PRIME DP - Unbound Medicine ER -