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Differential effect of nitric oxide synthase inhibitors on acetylcholine-induced relaxation of rat pulmonary and celiac artery rings.
Can J Physiol Pharmacol. 1997 Apr; 75(4):279-86.CJ

Abstract

The effect of NG-monomethyl-L-arginine (L-NMMA) and N omega-nitro-L-arginine methyl ester (L-NAME) on acetylcholine (ACh) induced relaxation of rat intrapulmonary artery and celiac artery ring segments was studied in vitro with and without meclofenamate pretreatment. L-NMMA, and to a lesser extent L-NAME, raised baseline tone more in pulmonary than celiac arteries. Pretreatment of pulmonary and celiac artery rings with meclofenamate did not alter this contractile effect. Pulmonary artery and celiac artery ring segments were precontracted with phenylephrine, and cumulative concentration-relaxation curves to ACh were obtained before and after incubation of the rings with L-NAME or L-NMMA. L-NAME inhibited the ACh-induced relaxation of pulmonary arterial rings at 10000 fold lower concentrations than those needed to only partly inhibit the ACh-induced relaxation of celiac artery rings. L-NMMA (10-300 microM) inhibited the ACh-induced relaxation of pulmonary arterial rings in a concentration-dependent manner, whereas L-NMMA (300 microM) only partially inhibited the ACh-induced relaxation of celiac artery rings. The inhibition of ACh-induced relaxation by L-NAME and to a lesser extent by L-NMMA was more when pulmonary and celiac artery rings were pretreated with meclofenamate (1.0 microM). These results suggest that in the pulmonary artery nitric oxide plays a greater role in the modulation of baseline vascular tone and ACh-induced relaxation than in the celiac artery. In addition, cyclooxygenase products do not contribute to the direct contractile responses to L-NAME and L-NMMA in both the pulmonary and celiac artery rings but do modulate the ACh-induced relaxation of these vessels.

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Authors+Show Affiliations

Yaghi A
A.C. Burton Vascular Biology Laboratory, Victoria Hospital, London Health Sciences Centre, Canada.
Paterson NA
No affiliation info available
McCormack DG
No affiliation info available

MeSH

AcetylcholineAnimalsCeliac ArteryCyclooxygenase InhibitorsEnzyme InhibitorsIn Vitro TechniquesMaleMeclofenamic AcidMicrocirculationNG-Nitroarginine Methyl EsterNitric Oxide SynthasePulmonary ArteryRatsRats, Sprague-DawleyVasodilationomega-N-Methylarginine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9196853

Citation

Yaghi, A, et al. "Differential Effect of Nitric Oxide Synthase Inhibitors On Acetylcholine-induced Relaxation of Rat Pulmonary and Celiac Artery Rings." Canadian Journal of Physiology and Pharmacology, vol. 75, no. 4, 1997, pp. 279-86.
Yaghi A, Paterson NA, McCormack DG. Differential effect of nitric oxide synthase inhibitors on acetylcholine-induced relaxation of rat pulmonary and celiac artery rings. Can J Physiol Pharmacol. 1997;75(4):279-86.
Yaghi, A., Paterson, N. A., & McCormack, D. G. (1997). Differential effect of nitric oxide synthase inhibitors on acetylcholine-induced relaxation of rat pulmonary and celiac artery rings. Canadian Journal of Physiology and Pharmacology, 75(4), 279-86.
Yaghi A, Paterson NA, McCormack DG. Differential Effect of Nitric Oxide Synthase Inhibitors On Acetylcholine-induced Relaxation of Rat Pulmonary and Celiac Artery Rings. Can J Physiol Pharmacol. 1997;75(4):279-86. PubMed PMID: 9196853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effect of nitric oxide synthase inhibitors on acetylcholine-induced relaxation of rat pulmonary and celiac artery rings. AU - Yaghi,A, AU - Paterson,N A, AU - McCormack,D G, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 279 EP - 86 JF - Canadian journal of physiology and pharmacology JO - Can J Physiol Pharmacol VL - 75 IS - 4 N2 - The effect of NG-monomethyl-L-arginine (L-NMMA) and N omega-nitro-L-arginine methyl ester (L-NAME) on acetylcholine (ACh) induced relaxation of rat intrapulmonary artery and celiac artery ring segments was studied in vitro with and without meclofenamate pretreatment. L-NMMA, and to a lesser extent L-NAME, raised baseline tone more in pulmonary than celiac arteries. Pretreatment of pulmonary and celiac artery rings with meclofenamate did not alter this contractile effect. Pulmonary artery and celiac artery ring segments were precontracted with phenylephrine, and cumulative concentration-relaxation curves to ACh were obtained before and after incubation of the rings with L-NAME or L-NMMA. L-NAME inhibited the ACh-induced relaxation of pulmonary arterial rings at 10000 fold lower concentrations than those needed to only partly inhibit the ACh-induced relaxation of celiac artery rings. L-NMMA (10-300 microM) inhibited the ACh-induced relaxation of pulmonary arterial rings in a concentration-dependent manner, whereas L-NMMA (300 microM) only partially inhibited the ACh-induced relaxation of celiac artery rings. The inhibition of ACh-induced relaxation by L-NAME and to a lesser extent by L-NMMA was more when pulmonary and celiac artery rings were pretreated with meclofenamate (1.0 microM). These results suggest that in the pulmonary artery nitric oxide plays a greater role in the modulation of baseline vascular tone and ACh-induced relaxation than in the celiac artery. In addition, cyclooxygenase products do not contribute to the direct contractile responses to L-NAME and L-NMMA in both the pulmonary and celiac artery rings but do modulate the ACh-induced relaxation of these vessels. SN - 0008-4212 UR - https://www.unboundmedicine.com/medline/citation/9196853/Differential_effect_of_nitric_oxide_synthase_inhibitors_on_acetylcholine_induced_relaxation_of_rat_pulmonary_and_celiac_artery_rings_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=9196853.ui DB - PRIME DP - Unbound Medicine ER -