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Detection of Pneumocystis carinii in tracheal aspirates of intubated patients using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) stains.
Crit Care Med. 1997 Jun; 25(6):948-52.CC

Abstract

OBJECTIVE

To compare the detection rate of Pneumocystis carinii in endotracheal aspirates with that rate in bronchoalveolar lavage fluid, using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) staining methods.

DESIGN

Prospective, consecutive cases.

SETTING

Medical intensive care unit at Ben Taub General Hospital.

PATIENTS

Thirty-one intubated patients admitted with respiratory failure and suspected P. carinii pneumonia.

INTERVENTIONS

An endotracheal aspirate specimen was obtained after maximally advancing a closed-system suction catheter, instilling aliquot portions of saline, and suctioning the lavage fluid. This procedure was followed within 30 mins by fiberoptic bronchoscopy and bronchoalveolar lavage.

MEASUREMENTS AND MAIN RESULTS

Endotracheal aspirate and bronchoalveolar lavage specimens from each patient were mixed with Saccomano's fixative, blended, and centrifuged. Using a modified method for P. carinii cysts, the sediment was stained with the test calcofluor-white stain Solution A and the test antibody stain. The test antibody stain on the bronchoalveolar lavage specimens was positive for P. carinii for 13 patients and was used as the standard for comparison. In the endotracheal aspirate specimens, the test antibody stain detected 12 (92%) P. carinii-positive patients while the test calcofluor-white stain detected ten (77%) P. carinii-positive patients.

CONCLUSIONS

We described a simple method for obtaining, processing, and staining endotracheal aspirate specimens for P. carinii. Obtaining an endotracheal aspirate specimen did not require specially trained personnel or a specialized and more expensive catheter, and was not associated with any complications.

Authors+Show Affiliations

Department of Medicine, Baylor College of Medicine, Houston, TX, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9201045

Citation

Alvarez, F, et al. "Detection of Pneumocystis Carinii in Tracheal Aspirates of Intubated Patients Using Calcofluor-white (Fungi-Fluor) and Immunofluorescence Antibody (Genetic Systems) Stains." Critical Care Medicine, vol. 25, no. 6, 1997, pp. 948-52.
Alvarez F, Bandi V, Stager C, et al. Detection of Pneumocystis carinii in tracheal aspirates of intubated patients using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) stains. Crit Care Med. 1997;25(6):948-52.
Alvarez, F., Bandi, V., Stager, C., & Guntupalli, K. K. (1997). Detection of Pneumocystis carinii in tracheal aspirates of intubated patients using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) stains. Critical Care Medicine, 25(6), 948-52.
Alvarez F, et al. Detection of Pneumocystis Carinii in Tracheal Aspirates of Intubated Patients Using Calcofluor-white (Fungi-Fluor) and Immunofluorescence Antibody (Genetic Systems) Stains. Crit Care Med. 1997;25(6):948-52. PubMed PMID: 9201045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Pneumocystis carinii in tracheal aspirates of intubated patients using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) stains. AU - Alvarez,F, AU - Bandi,V, AU - Stager,C, AU - Guntupalli,K K, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 948 EP - 52 JF - Critical care medicine JO - Crit Care Med VL - 25 IS - 6 N2 - OBJECTIVE: To compare the detection rate of Pneumocystis carinii in endotracheal aspirates with that rate in bronchoalveolar lavage fluid, using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) staining methods. DESIGN: Prospective, consecutive cases. SETTING: Medical intensive care unit at Ben Taub General Hospital. PATIENTS: Thirty-one intubated patients admitted with respiratory failure and suspected P. carinii pneumonia. INTERVENTIONS: An endotracheal aspirate specimen was obtained after maximally advancing a closed-system suction catheter, instilling aliquot portions of saline, and suctioning the lavage fluid. This procedure was followed within 30 mins by fiberoptic bronchoscopy and bronchoalveolar lavage. MEASUREMENTS AND MAIN RESULTS: Endotracheal aspirate and bronchoalveolar lavage specimens from each patient were mixed with Saccomano's fixative, blended, and centrifuged. Using a modified method for P. carinii cysts, the sediment was stained with the test calcofluor-white stain Solution A and the test antibody stain. The test antibody stain on the bronchoalveolar lavage specimens was positive for P. carinii for 13 patients and was used as the standard for comparison. In the endotracheal aspirate specimens, the test antibody stain detected 12 (92%) P. carinii-positive patients while the test calcofluor-white stain detected ten (77%) P. carinii-positive patients. CONCLUSIONS: We described a simple method for obtaining, processing, and staining endotracheal aspirate specimens for P. carinii. Obtaining an endotracheal aspirate specimen did not require specially trained personnel or a specialized and more expensive catheter, and was not associated with any complications. SN - 0090-3493 UR - https://www.unboundmedicine.com/medline/citation/9201045/Detection_of_Pneumocystis_carinii_in_tracheal_aspirates_of_intubated_patients_using_calcofluor_white__Fungi_Fluor__and_immunofluorescence_antibody__Genetic_Systems__stains_ L2 - https://dx.doi.org/10.1097/00003246-199706000-00009 DB - PRIME DP - Unbound Medicine ER -