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CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica.
Microbiology (Reading). 1997 Jun; 143 (Pt 6):2009-20.M

Abstract

PCR was used to amplify fragments corresponding to the chitin synthase (CHS) genes from the Oomycetes Saprolegnia monoica, Phytophthora capsici and Achlya ambisexualis, utilizing as primers, oligonucleotides designed from the conserved region of CHS genes of chitinous fungi. Chitin synthase homologues were found in the three cellulosic fungi. The chitin synthase 2 gene (CHS2) from S. monoica was cloned, sequenced and characterized. The amino acid sequence deduced from the CHS2 genomic DNA revealed several domains, corresponding to the catalytic domains and polypeptide signatures, of high identity with CHS genes from chitinous fungi. Existence of a CHS gene family in S. monoica was supported by the identification of two CHS sequences among the PCR products, the localization of CHS homologues on two chromosomes, and the detection of two transcripts in mycelia and protoplasts. Polyclonal anti-chitin synthase antibodies raised against the N-terminal and the neutral fragments of the CHS2 products revealed, respectively, two and four proteins in membrane fractions and a truncated active form in entrapped product. The overall comparison of the structure and organization of CHS genes indicates that in spite of their divergent evolution, Oomycetes and chitinous fungi have evolved with conserved chitin synthase systems.

Authors+Show Affiliations

Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 5534, Université Lyon, Villeurbanne, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9202477

Citation

Mort-Bontemps, M, et al. "CHS2, a Chitin Synthase Gene From the Oomycete Saprolegnia Monoica." Microbiology (Reading, England), vol. 143 (Pt 6), 1997, pp. 2009-20.
Mort-Bontemps M, Gay L, Fèvre M. CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica. Microbiology (Reading). 1997;143 (Pt 6):2009-20.
Mort-Bontemps, M., Gay, L., & Fèvre, M. (1997). CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica. Microbiology (Reading, England), 143 (Pt 6), 2009-20.
Mort-Bontemps M, Gay L, Fèvre M. CHS2, a Chitin Synthase Gene From the Oomycete Saprolegnia Monoica. Microbiology (Reading). 1997;143 (Pt 6):2009-20. PubMed PMID: 9202477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CHS2, a chitin synthase gene from the oomycete Saprolegnia monoica. AU - Mort-Bontemps,M, AU - Gay,L, AU - Fèvre,M, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 2009 EP - 20 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 143 (Pt 6) N2 - PCR was used to amplify fragments corresponding to the chitin synthase (CHS) genes from the Oomycetes Saprolegnia monoica, Phytophthora capsici and Achlya ambisexualis, utilizing as primers, oligonucleotides designed from the conserved region of CHS genes of chitinous fungi. Chitin synthase homologues were found in the three cellulosic fungi. The chitin synthase 2 gene (CHS2) from S. monoica was cloned, sequenced and characterized. The amino acid sequence deduced from the CHS2 genomic DNA revealed several domains, corresponding to the catalytic domains and polypeptide signatures, of high identity with CHS genes from chitinous fungi. Existence of a CHS gene family in S. monoica was supported by the identification of two CHS sequences among the PCR products, the localization of CHS homologues on two chromosomes, and the detection of two transcripts in mycelia and protoplasts. Polyclonal anti-chitin synthase antibodies raised against the N-terminal and the neutral fragments of the CHS2 products revealed, respectively, two and four proteins in membrane fractions and a truncated active form in entrapped product. The overall comparison of the structure and organization of CHS genes indicates that in spite of their divergent evolution, Oomycetes and chitinous fungi have evolved with conserved chitin synthase systems. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/9202477/CHS2_a_chitin_synthase_gene_from_the_oomycete_Saprolegnia_monoica_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/00221287-143-6-2009 DB - PRIME DP - Unbound Medicine ER -