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A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13.
Genomics. 1997 Jun 15; 42(3):436-45.G

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS-content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FISH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region.

Authors+Show Affiliations

Laboratory of Gene Transfer, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9205115

Citation

Guru, S C., et al. "A 2.8-Mb Clone Contig of the Multiple Endocrine Neoplasia Type 1 (MEN1) Region at 11q13." Genomics, vol. 42, no. 3, 1997, pp. 436-45.
Guru SC, Olufemi SE, Manickam P, et al. A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13. Genomics. 1997;42(3):436-45.
Guru, S. C., Olufemi, S. E., Manickam, P., Cummings, C., Gieser, L. M., Pike, B. L., Bittner, M. L., Jiang, Y., Chinault, A. C., Nowak, N. J., Brzozowska, A., Crabtree, J. S., Wang, Y., Roe, B. A., Weisemann, J. M., Boguski, M. S., Agarwal, S. K., Burns, A. L., Spiegel, A. M., ... Chandrasekharappa, S. C. (1997). A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13. Genomics, 42(3), 436-45.
Guru SC, et al. A 2.8-Mb Clone Contig of the Multiple Endocrine Neoplasia Type 1 (MEN1) Region at 11q13. Genomics. 1997 Jun 15;42(3):436-45. PubMed PMID: 9205115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13. AU - Guru,S C, AU - Olufemi,S E, AU - Manickam,P, AU - Cummings,C, AU - Gieser,L M, AU - Pike,B L, AU - Bittner,M L, AU - Jiang,Y, AU - Chinault,A C, AU - Nowak,N J, AU - Brzozowska,A, AU - Crabtree,J S, AU - Wang,Y, AU - Roe,B A, AU - Weisemann,J M, AU - Boguski,M S, AU - Agarwal,S K, AU - Burns,A L, AU - Spiegel,A M, AU - Marx,S J, AU - Flejter,W L, AU - de Jong,P J, AU - Collins,F S, AU - Chandrasekharappa,S C, PY - 1997/6/15/pubmed PY - 1997/6/15/medline PY - 1997/6/15/entrez SP - 436 EP - 45 JF - Genomics JO - Genomics VL - 42 IS - 3 N2 - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS-content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FISH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region. SN - 0888-7543 UR - https://www.unboundmedicine.com/medline/citation/9205115/A_2_8_Mb_clone_contig_of_the_multiple_endocrine_neoplasia_type_1__MEN1__region_at_11q13_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0888-7543(97)94783-2 DB - PRIME DP - Unbound Medicine ER -