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Modification of odd-chain length unsaturated fatty acids by hepatocytes of rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil.
Lipids 1997; 32(6):611-9L

Abstract

Hepatocytes isolated from rainbow trout fed on diets containing either fish oil or olive oil were incubated with individual odd-chain length unsaturated fatty acids (19:1n-9, 19:2n-6, 19:3n-3, 21:2n-6, 21:3n-6, 21:4n-6, 21:3n-3, and 21:5n-3) to examine whether these fatty acids were substrates for modification by desaturation and elongation. All odd-chain length fatty acids were readily assimilated into the lipids of hepatocytes from both dietary groups of fish, but their conversion to longer-chain, more unsaturated derivatives was more pronounced with cells from trout fed olive oil. Thus, the conversion of 19:2n-6 and 21:2n-6 to 21:3n-6 and 21:4n-6, and of 19:3n-3 to 21:4n-3 and 21:5n-3, was most obvious in cells from the olive oil group, as was the conversion of 21:3n-6 and 21:3n-3 to 21:4n-6 and 21:4n-3, respectively. Elongation of 19:1n-9 to 21:1n-9 and 23:1n-9 occurred in cells from both groups. No 23:6n-3 was detectable as a product of 19:3n-3 or 21:3n-3. However, this fatty acid was a major product formed by cells from fish fed olive oil presented with 21:5n-3. Cells from both groups of fish incorporated 21:4n-6 and 21:5n-3 into their lipids largely without modification but chain-shortened around 40, 23, and 19% of the incorporated 21:2n-6, 21:3n-3, and 19:1n-9, respectively. The results demonstrate that odd-chain length unsaturated fatty acids can act as substrates for the desaturation, elongation, and chain-shortening systems of trout hepatocytes.

Authors+Show Affiliations

N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Sciences, University of Stirling, Scotland, United Kingdom. covarodr@ull.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9208390

Citation

Rodríguez, C, et al. "Modification of Odd-chain Length Unsaturated Fatty Acids By Hepatocytes of Rainbow Trout (Oncorhynchus Mykiss) Fed Diets Containing Fish Oil or Olive Oil." Lipids, vol. 32, no. 6, 1997, pp. 611-9.
Rodríguez C, Henderson RJ, Porter AE, et al. Modification of odd-chain length unsaturated fatty acids by hepatocytes of rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil. Lipids. 1997;32(6):611-9.
Rodríguez, C., Henderson, R. J., Porter, A. E., & Dick, J. R. (1997). Modification of odd-chain length unsaturated fatty acids by hepatocytes of rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil. Lipids, 32(6), pp. 611-9.
Rodríguez C, et al. Modification of Odd-chain Length Unsaturated Fatty Acids By Hepatocytes of Rainbow Trout (Oncorhynchus Mykiss) Fed Diets Containing Fish Oil or Olive Oil. Lipids. 1997;32(6):611-9. PubMed PMID: 9208390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modification of odd-chain length unsaturated fatty acids by hepatocytes of rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil. AU - Rodríguez,C, AU - Henderson,R J, AU - Porter,A E, AU - Dick,J R, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 611 EP - 9 JF - Lipids JO - Lipids VL - 32 IS - 6 N2 - Hepatocytes isolated from rainbow trout fed on diets containing either fish oil or olive oil were incubated with individual odd-chain length unsaturated fatty acids (19:1n-9, 19:2n-6, 19:3n-3, 21:2n-6, 21:3n-6, 21:4n-6, 21:3n-3, and 21:5n-3) to examine whether these fatty acids were substrates for modification by desaturation and elongation. All odd-chain length fatty acids were readily assimilated into the lipids of hepatocytes from both dietary groups of fish, but their conversion to longer-chain, more unsaturated derivatives was more pronounced with cells from trout fed olive oil. Thus, the conversion of 19:2n-6 and 21:2n-6 to 21:3n-6 and 21:4n-6, and of 19:3n-3 to 21:4n-3 and 21:5n-3, was most obvious in cells from the olive oil group, as was the conversion of 21:3n-6 and 21:3n-3 to 21:4n-6 and 21:4n-3, respectively. Elongation of 19:1n-9 to 21:1n-9 and 23:1n-9 occurred in cells from both groups. No 23:6n-3 was detectable as a product of 19:3n-3 or 21:3n-3. However, this fatty acid was a major product formed by cells from fish fed olive oil presented with 21:5n-3. Cells from both groups of fish incorporated 21:4n-6 and 21:5n-3 into their lipids largely without modification but chain-shortened around 40, 23, and 19% of the incorporated 21:2n-6, 21:3n-3, and 19:1n-9, respectively. The results demonstrate that odd-chain length unsaturated fatty acids can act as substrates for the desaturation, elongation, and chain-shortening systems of trout hepatocytes. SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/9208390/Modification_of_odd_chain_length_unsaturated_fatty_acids_by_hepatocytes_of_rainbow_trout__Oncorhynchus_mykiss__fed_diets_containing_fish_oil_or_olive_oil_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0024-4201&date=1997&volume=32&issue=6&spage=611 DB - PRIME DP - Unbound Medicine ER -