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Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys by in situ hybridization.
Int J Cancer. 1997 Jul 03; 72(1):160-5.IJ

Abstract

SIV infection of macaques is a well-established animal model for studying the pathogenesis of HIV infection in humans. During the course of SIV infection, up to 40% of cynomolgus macaques (Macaca fascicularis) develop SIV-associated non-Hodgkin's lymphomas. In the present study, we characterized malignant lymphomas of SIV(mac) 251/32H-infected rhesus macaques (Macaca mulatto) of our cohort in terms of clinical outcome, histopathology and EBV association. Histopathologic changes of lymphoid malignancies were classified according to the Kiel classification. For detection of the EBV-encoded small RNAs EBER1 and EBER2, a method of non-isotopic in situ hybridization was established. The presence of EBNA-2 antigens was assessed by immunohistochemistry. Seven of 43 rhesus macaques developed highly malignant B-cell lymphomas of the centroblastic, immunoblastic and Burkitt subtypes within 18-29 months post-experimental SIV infection. In situ hybridization revealed the presence of small EBER1 and -2 RNAs in 6 of 7 disease cases. EBNA-2 antigens could be demonstrated in only 4 of 7 tissue specimens. As expected, the Burkitt-type of lymphoma was negative for EBNA-2 antigen staining. In accordance with findings on SIV-associated lymphomas of cynomolgus macaques, infection with an EBV-related herpesvirus could be demonstrated in almost 90% of lymphomas in SIV-infected rhesus macaques. In contrast, the presence of EBV in lymphomas had been documented previously in only 30-40% of HIV-infected patients. Further studies should thus define the precise role of herpesvirus infection for lymphomagenesis in SIV- and HIV-induced immunodeficiency.

Authors+Show Affiliations

Department of Virology/Immunology, German Primate Center, Göttingen.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9212238

Citation

Pingel, S, et al. "Detection of Epstein-Barr Virus Small RNAs EBER1 and EBER2 in Lymphomas of SIV-infected Rhesus Monkeys By in Situ Hybridization." International Journal of Cancer, vol. 72, no. 1, 1997, pp. 160-5.
Pingel S, Hannig H, Mätz-Rensing K, et al. Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys by in situ hybridization. Int J Cancer. 1997;72(1):160-5.
Pingel, S., Hannig, H., Mätz-Rensing, K., Kaup, F. J., Hunsmann, G., & Bodemer, W. (1997). Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys by in situ hybridization. International Journal of Cancer, 72(1), 160-5.
Pingel S, et al. Detection of Epstein-Barr Virus Small RNAs EBER1 and EBER2 in Lymphomas of SIV-infected Rhesus Monkeys By in Situ Hybridization. Int J Cancer. 1997 Jul 3;72(1):160-5. PubMed PMID: 9212238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys by in situ hybridization. AU - Pingel,S, AU - Hannig,H, AU - Mätz-Rensing,K, AU - Kaup,F J, AU - Hunsmann,G, AU - Bodemer,W, PY - 1997/7/3/pubmed PY - 2000/6/20/medline PY - 1997/7/3/entrez SP - 160 EP - 5 JF - International journal of cancer JO - Int J Cancer VL - 72 IS - 1 N2 - SIV infection of macaques is a well-established animal model for studying the pathogenesis of HIV infection in humans. During the course of SIV infection, up to 40% of cynomolgus macaques (Macaca fascicularis) develop SIV-associated non-Hodgkin's lymphomas. In the present study, we characterized malignant lymphomas of SIV(mac) 251/32H-infected rhesus macaques (Macaca mulatto) of our cohort in terms of clinical outcome, histopathology and EBV association. Histopathologic changes of lymphoid malignancies were classified according to the Kiel classification. For detection of the EBV-encoded small RNAs EBER1 and EBER2, a method of non-isotopic in situ hybridization was established. The presence of EBNA-2 antigens was assessed by immunohistochemistry. Seven of 43 rhesus macaques developed highly malignant B-cell lymphomas of the centroblastic, immunoblastic and Burkitt subtypes within 18-29 months post-experimental SIV infection. In situ hybridization revealed the presence of small EBER1 and -2 RNAs in 6 of 7 disease cases. EBNA-2 antigens could be demonstrated in only 4 of 7 tissue specimens. As expected, the Burkitt-type of lymphoma was negative for EBNA-2 antigen staining. In accordance with findings on SIV-associated lymphomas of cynomolgus macaques, infection with an EBV-related herpesvirus could be demonstrated in almost 90% of lymphomas in SIV-infected rhesus macaques. In contrast, the presence of EBV in lymphomas had been documented previously in only 30-40% of HIV-infected patients. Further studies should thus define the precise role of herpesvirus infection for lymphomagenesis in SIV- and HIV-induced immunodeficiency. SN - 0020-7136 UR - https://www.unboundmedicine.com/medline/citation/9212238/Detection_of_Epstein_Barr_virus_small_RNAs_EBER1_and_EBER2_in_lymphomas_of_SIV_infected_rhesus_monkeys_by_in_situ_hybridization_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0020-7136&date=1997&volume=72&issue=1&spage=160 DB - PRIME DP - Unbound Medicine ER -