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Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.
Plant Cell. 1997 Jun; 9(6):909-23.PC

Abstract

A putative yeast sn-2 acyltransferase gene (SLC1-1), reportedly a variant acyltransferase that suppresses a genetic defect in sphingolipid long-chain base biosynthesis, has been expressed in a yeast SLC deletion strain. The SLC1-1 gene product was shown in vitro to encode an sn-2 acyltransferase capable of acylating sn-1 oleoyl-lysophosphatidic acid, using a range of acyl-CoA thioesters, including 18:1-, 22:1-, and 24:0-CoAs. The SLC1-1 gene was introduced into Arabidopsis and a high erucic acid-containing Brassica napus cv Hero under the control of a constitutive (tandem cauliflower mosaic virus 35S) promoter. The resulting transgenic plants showed substantial increases of 8 to 48% in seed oil content (expressed on the basis of seed dry weight) and increases in both overall proportions and amounts of very-long-chain fatty acids in seed triacylglycerols (TAGs). Furthermore, the proportion of very-long-chain fatty acids found at the sn-2 position of TAGs was increased, and homogenates prepared from developing seeds of transformed plants exhibited elevated lysophosphatidic acid acyltransferase (EC 2.3.1.51) activity. Thus, the yeast sn-2 acyltransferase has been shown to encode a protein that can exhibit lysophosphatidic acid acyltransferase activity and that can be used to change total fatty acid content and composition as well as to alter the stereospecific acyl distribution of fatty acids in seed TAGs.

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Authors+Show Affiliations

Zou J
National Research Council of Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada.
Katavic V
No affiliation info available
Giblin EM
No affiliation info available
Barton DL
No affiliation info available
MacKenzie SL
No affiliation info available
Keller WA
No affiliation info available
Hu X
No affiliation info available
Taylor DC
No affiliation info available

MeSH

1-Acylglycerol-3-Phosphate O-AcyltransferaseAcylationAcyltransferasesAllelesArabidopsisBrassicaCloning, MolecularDyneinsFatty AcidsFungal ProteinsGenes, PlantGenes, SuppressorPlant OilsPlants, Genetically ModifiedSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSeedsTriglycerides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9212466

Citation

Zou, J, et al. "Modification of Seed Oil Content and Acyl Composition in the Brassicaceae By Expression of a Yeast Sn-2 Acyltransferase Gene." The Plant Cell, vol. 9, no. 6, 1997, pp. 909-23.
Zou J, Katavic V, Giblin EM, et al. Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell. 1997;9(6):909-23.
Zou, J., Katavic, V., Giblin, E. M., Barton, D. L., MacKenzie, S. L., Keller, W. A., Hu, X., & Taylor, D. C. (1997). Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. The Plant Cell, 9(6), 909-23.
Zou J, et al. Modification of Seed Oil Content and Acyl Composition in the Brassicaceae By Expression of a Yeast Sn-2 Acyltransferase Gene. Plant Cell. 1997;9(6):909-23. PubMed PMID: 9212466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. AU - Zou,J, AU - Katavic,V, AU - Giblin,E M, AU - Barton,D L, AU - MacKenzie,S L, AU - Keller,W A, AU - Hu,X, AU - Taylor,D C, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 909 EP - 23 JF - The Plant cell JO - Plant Cell VL - 9 IS - 6 N2 - A putative yeast sn-2 acyltransferase gene (SLC1-1), reportedly a variant acyltransferase that suppresses a genetic defect in sphingolipid long-chain base biosynthesis, has been expressed in a yeast SLC deletion strain. The SLC1-1 gene product was shown in vitro to encode an sn-2 acyltransferase capable of acylating sn-1 oleoyl-lysophosphatidic acid, using a range of acyl-CoA thioesters, including 18:1-, 22:1-, and 24:0-CoAs. The SLC1-1 gene was introduced into Arabidopsis and a high erucic acid-containing Brassica napus cv Hero under the control of a constitutive (tandem cauliflower mosaic virus 35S) promoter. The resulting transgenic plants showed substantial increases of 8 to 48% in seed oil content (expressed on the basis of seed dry weight) and increases in both overall proportions and amounts of very-long-chain fatty acids in seed triacylglycerols (TAGs). Furthermore, the proportion of very-long-chain fatty acids found at the sn-2 position of TAGs was increased, and homogenates prepared from developing seeds of transformed plants exhibited elevated lysophosphatidic acid acyltransferase (EC 2.3.1.51) activity. Thus, the yeast sn-2 acyltransferase has been shown to encode a protein that can exhibit lysophosphatidic acid acyltransferase activity and that can be used to change total fatty acid content and composition as well as to alter the stereospecific acyl distribution of fatty acids in seed TAGs. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/9212466/Modification_of_seed_oil_content_and_acyl_composition_in_the_brassicaceae_by_expression_of_a_yeast_sn_2_acyltransferase_gene_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=9212466 DB - PRIME DP - Unbound Medicine ER -