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Isolation of a cDNA encoding a tyrosine kinase expressed in murine skin.
Exp Dermatol 1997; 6(3):140-6ED

Abstract

Tyrosine phosphorylation is widely recognized as playing an important role in cell differentiation, proliferation and carcinogenesis. We used the polymerase chain reaction (PCR) method to identify protein tyrosine kinases that were expressed in the skin. Mixed oligonucleotide probes were used to amplify and screen neonatal murine skin mRNA for clones encoding amino acid contiguities, the conservation of which is characteristic of the protein tyrosine kinase family. When the PCR products were sequenced, a novel clone encoding protein tyrosine kinase, PTK70, was identified. A full-length cDNA was isolated from a mouse thymus cDNA library. The nucleotide and deduced amino acid sequence showed that it featured src-homology (SH) 2 domain, SH3 domain and kinase domain like other src family protein tyrosine kinases, but lacked the N-terminal myristylation site and C-terminal tyrosine residue. Although the mRNA of PTK70 was detected in various tissues ubiquitously, the degree of its expression differed among tissues. Murine skin is one in which PTK70 was expressed strongly, with its expression being much stronger in the epidermis and in the cell line derived from murine keratinocytes than in those from melanoma or fibroblast cell lines. These evidences suggest that PTK70 may be involved in proliferation or differentiation of keratinocytes in the skin.

Authors+Show Affiliations

Department of Dermatology, University of Tsukuba, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9226137

Citation

Kawachi, Y, et al. "Isolation of a cDNA Encoding a Tyrosine Kinase Expressed in Murine Skin." Experimental Dermatology, vol. 6, no. 3, 1997, pp. 140-6.
Kawachi Y, Nakauchi H, Otsuka F. Isolation of a cDNA encoding a tyrosine kinase expressed in murine skin. Exp Dermatol. 1997;6(3):140-6.
Kawachi, Y., Nakauchi, H., & Otsuka, F. (1997). Isolation of a cDNA encoding a tyrosine kinase expressed in murine skin. Experimental Dermatology, 6(3), pp. 140-6.
Kawachi Y, Nakauchi H, Otsuka F. Isolation of a cDNA Encoding a Tyrosine Kinase Expressed in Murine Skin. Exp Dermatol. 1997;6(3):140-6. PubMed PMID: 9226137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of a cDNA encoding a tyrosine kinase expressed in murine skin. AU - Kawachi,Y, AU - Nakauchi,H, AU - Otsuka,F, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 140 EP - 6 JF - Experimental dermatology JO - Exp. Dermatol. VL - 6 IS - 3 N2 - Tyrosine phosphorylation is widely recognized as playing an important role in cell differentiation, proliferation and carcinogenesis. We used the polymerase chain reaction (PCR) method to identify protein tyrosine kinases that were expressed in the skin. Mixed oligonucleotide probes were used to amplify and screen neonatal murine skin mRNA for clones encoding amino acid contiguities, the conservation of which is characteristic of the protein tyrosine kinase family. When the PCR products were sequenced, a novel clone encoding protein tyrosine kinase, PTK70, was identified. A full-length cDNA was isolated from a mouse thymus cDNA library. The nucleotide and deduced amino acid sequence showed that it featured src-homology (SH) 2 domain, SH3 domain and kinase domain like other src family protein tyrosine kinases, but lacked the N-terminal myristylation site and C-terminal tyrosine residue. Although the mRNA of PTK70 was detected in various tissues ubiquitously, the degree of its expression differed among tissues. Murine skin is one in which PTK70 was expressed strongly, with its expression being much stronger in the epidermis and in the cell line derived from murine keratinocytes than in those from melanoma or fibroblast cell lines. These evidences suggest that PTK70 may be involved in proliferation or differentiation of keratinocytes in the skin. SN - 0906-6705 UR - https://www.unboundmedicine.com/medline/citation/9226137/Isolation_of_a_cDNA_encoding_a_tyrosine_kinase_expressed_in_murine_skin_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0906-6705&date=1997&volume=6&issue=3&spage=140 DB - PRIME DP - Unbound Medicine ER -