Tags

Type your tag names separated by a space and hit enter

Measurement of NO synthesis in humans by L-[15N2]arginine: application to the response to vaccination.
Am J Physiol. 1997 Jun; 272(6 Pt 2):R1888-96.AJ

Abstract

Induction of nitric oxide (NO) synthesis is a key element of the inflammatory response in humans. We describe a sensitive gas isotope ratio mass spectrometric (GIRMS) method for measuring urinary [15N]nitrate production during intravenous infusion of L-[guanidino-15N2]arginine and its application to investigate the effects of a controlled inflammatory stimulus, typhoid vaccination, on NO synthesis in humans. Intravenous infusion of L-[15N2]arginine at 5-12 mumol.kg-1.h-1 for 24 h in three subjects was used to determine arginine and nitrate pool kinetics. Eight subjects received primed constant infusion of 2.5 mumol.kg-1.h-1 of L-[15N2]arginine for 12 h once before and again after typhoid vaccination. NO synthesis was calculated from 15N enrichment of plasma arginine and urinary nitrate, measured by gas chromatography mass spectrometry and GIRMS, respectively, and total urinary nitrate excretion. Baseline NO synthesis was 298 +/- 44 nmol.h-1.kg lean body mass-1, representing 0.41% of arginine flux. After vaccination, NO synthesis (267 +/- 77 nmol.h-1.kg-1) was not increased (P = 0.18), despite demonstration of an acute phase response. Typhoid vaccination is not accompanied by accelerated NO synthesis.

Authors+Show Affiliations

Division of Infectious Diseases, Saint George's Hospital Medical School, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9227604

Citation

Macallan, D C., et al. "Measurement of NO Synthesis in Humans By L-[15N2]arginine: Application to the Response to Vaccination." The American Journal of Physiology, vol. 272, no. 6 Pt 2, 1997, pp. R1888-96.
Macallan DC, Smith LM, Ferber J, et al. Measurement of NO synthesis in humans by L-[15N2]arginine: application to the response to vaccination. Am J Physiol. 1997;272(6 Pt 2):R1888-96.
Macallan, D. C., Smith, L. M., Ferber, J., Milne, E., Griffin, G. E., Benjamin, N., & McNurlan, M. A. (1997). Measurement of NO synthesis in humans by L-[15N2]arginine: application to the response to vaccination. The American Journal of Physiology, 272(6 Pt 2), R1888-96.
Macallan DC, et al. Measurement of NO Synthesis in Humans By L-[15N2]arginine: Application to the Response to Vaccination. Am J Physiol. 1997;272(6 Pt 2):R1888-96. PubMed PMID: 9227604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of NO synthesis in humans by L-[15N2]arginine: application to the response to vaccination. AU - Macallan,D C, AU - Smith,L M, AU - Ferber,J, AU - Milne,E, AU - Griffin,G E, AU - Benjamin,N, AU - McNurlan,M A, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - R1888 EP - 96 JF - The American journal of physiology JO - Am. J. Physiol. VL - 272 IS - 6 Pt 2 N2 - Induction of nitric oxide (NO) synthesis is a key element of the inflammatory response in humans. We describe a sensitive gas isotope ratio mass spectrometric (GIRMS) method for measuring urinary [15N]nitrate production during intravenous infusion of L-[guanidino-15N2]arginine and its application to investigate the effects of a controlled inflammatory stimulus, typhoid vaccination, on NO synthesis in humans. Intravenous infusion of L-[15N2]arginine at 5-12 mumol.kg-1.h-1 for 24 h in three subjects was used to determine arginine and nitrate pool kinetics. Eight subjects received primed constant infusion of 2.5 mumol.kg-1.h-1 of L-[15N2]arginine for 12 h once before and again after typhoid vaccination. NO synthesis was calculated from 15N enrichment of plasma arginine and urinary nitrate, measured by gas chromatography mass spectrometry and GIRMS, respectively, and total urinary nitrate excretion. Baseline NO synthesis was 298 +/- 44 nmol.h-1.kg lean body mass-1, representing 0.41% of arginine flux. After vaccination, NO synthesis (267 +/- 77 nmol.h-1.kg-1) was not increased (P = 0.18), despite demonstration of an acute phase response. Typhoid vaccination is not accompanied by accelerated NO synthesis. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/9227604/Measurement_of_NO_synthesis_in_humans_by_L_[15N2]arginine:_application_to_the_response_to_vaccination_ L2 - http://journals.physiology.org/doi/full/10.1152/ajpregu.1997.272.6.R1888?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -