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A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes.
J Immunol. 1997 Aug 01; 159(3):1140-9.JI

Abstract

By searching the expressed sequence tag (EST) database, we identified partial cDNA sequences encoding a polypeptide with significant sequence identity to the human CC chemokine macrophage-inflammatory protein-1 alpha (MIP-1 alpha)/LD78 alpha. We determined the complete cDNA sequence that contained a reading frame of 89 amino acids with 61% identity to human MIP-1 alpha/LD78 alpha. The mRNA was expressed constitutively at high levels in human lung and at low levels in some lymphoid tissues. Furthermore, the mRNA was strongly induced in several human cell lines, including monocytic U937 cells, by PMA. From these results, we designated this novel CC chemokine as PARC from pulmonary and activation-regulated chemokine. In situ hybridization analyses showed that alveolar macrophages, follicular dendritic cells in the germinal centers of regional lymph nodes, and peripheral blood monocytes stimulated with LPS express PARC mRNA. Using the human CC chemokine yeast artificial chromosome contig that we constructed recently, we mapped the PARC gene (SCYA18) within one of the two subregions of the CC chemokine gene cluster at chromosome 17q11.2. To investigate its biologic activity, the PARC protein was expressed in insect cells. PARC was chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Binding analysis using PARC fused with alkaline phosphatase-(His)6 showed the presence of a single class of receptors for PARC on lymphocytes with a Kd of 1.9 nM and 590 sites/cell. Thus, PARC is a novel CC chemokine with a close phylogenic relationship with MIP-1 alpha/LD78 alpha, but with a highly selective activity on lymphocytes.

Authors+Show Affiliations

Department of Biochemistry, Kumamoto University Medical School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9233607

Citation

Hieshima, K, et al. "A Novel Human CC Chemokine PARC That Is Most Homologous to Macrophage-inflammatory Protein-1 alpha/LD78 Alpha and Chemotactic for T Lymphocytes, but Not for Monocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 159, no. 3, 1997, pp. 1140-9.
Hieshima K, Imai T, Baba M, et al. A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes. J Immunol. 1997;159(3):1140-9.
Hieshima, K., Imai, T., Baba, M., Shoudai, K., Ishizuka, K., Nakagawa, T., Tsuruta, J., Takeya, M., Sakaki, Y., Takatsuki, K., Miura, R., Opdenakker, G., Van Damme, J., Yoshie, O., & Nomiyama, H. (1997). A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes. Journal of Immunology (Baltimore, Md. : 1950), 159(3), 1140-9.
Hieshima K, et al. A Novel Human CC Chemokine PARC That Is Most Homologous to Macrophage-inflammatory Protein-1 alpha/LD78 Alpha and Chemotactic for T Lymphocytes, but Not for Monocytes. J Immunol. 1997 Aug 1;159(3):1140-9. PubMed PMID: 9233607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel human CC chemokine PARC that is most homologous to macrophage-inflammatory protein-1 alpha/LD78 alpha and chemotactic for T lymphocytes, but not for monocytes. AU - Hieshima,K, AU - Imai,T, AU - Baba,M, AU - Shoudai,K, AU - Ishizuka,K, AU - Nakagawa,T, AU - Tsuruta,J, AU - Takeya,M, AU - Sakaki,Y, AU - Takatsuki,K, AU - Miura,R, AU - Opdenakker,G, AU - Van Damme,J, AU - Yoshie,O, AU - Nomiyama,H, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 1140 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 159 IS - 3 N2 - By searching the expressed sequence tag (EST) database, we identified partial cDNA sequences encoding a polypeptide with significant sequence identity to the human CC chemokine macrophage-inflammatory protein-1 alpha (MIP-1 alpha)/LD78 alpha. We determined the complete cDNA sequence that contained a reading frame of 89 amino acids with 61% identity to human MIP-1 alpha/LD78 alpha. The mRNA was expressed constitutively at high levels in human lung and at low levels in some lymphoid tissues. Furthermore, the mRNA was strongly induced in several human cell lines, including monocytic U937 cells, by PMA. From these results, we designated this novel CC chemokine as PARC from pulmonary and activation-regulated chemokine. In situ hybridization analyses showed that alveolar macrophages, follicular dendritic cells in the germinal centers of regional lymph nodes, and peripheral blood monocytes stimulated with LPS express PARC mRNA. Using the human CC chemokine yeast artificial chromosome contig that we constructed recently, we mapped the PARC gene (SCYA18) within one of the two subregions of the CC chemokine gene cluster at chromosome 17q11.2. To investigate its biologic activity, the PARC protein was expressed in insect cells. PARC was chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Binding analysis using PARC fused with alkaline phosphatase-(His)6 showed the presence of a single class of receptors for PARC on lymphocytes with a Kd of 1.9 nM and 590 sites/cell. Thus, PARC is a novel CC chemokine with a close phylogenic relationship with MIP-1 alpha/LD78 alpha, but with a highly selective activity on lymphocytes. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9233607/A_novel_human_CC_chemokine_PARC_that_is_most_homologous_to_macrophage_inflammatory_protein_1_alpha/LD78_alpha_and_chemotactic_for_T_lymphocytes_but_not_for_monocytes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9233607 DB - PRIME DP - Unbound Medicine ER -