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Bcl-2 and Hsp27 act at different levels to suppress programmed cell death.
Oncogene 1997; 15(3):347-60O

Abstract

Apoptosis and necrosis, two morphologically distinct forms of cell death, can be induced by common stimuli depending on the doses and the cell type. This study compares the protective effect of oncoprotein Bcl-2 and of the small stress protein Hsp27 on these two types of cell death. We use rat embryo fibroblasts conditionally immortalized by the tsA58 mutant of SV40 large T antigen as parental cells to develop cell lines carrying inducible bcl-2 or hsp27 genes. Two apoptotic stimuli were used: shift to the restrictive temperature that induced p53-mediated apoptosis and treatment with low doses of hydrogen peroxide. Necrosis was induced by high doses of hydrogen peroxide. Although Bcl-2 and Hsp27 protect these cells from necrotic death, only Bcl-2 appears capable of preventing apoptotic death. Bcl-2 protection is not mediated by a negative effect on the induction of the p53 responsive genes bax or waf1 but it slows down at least two stages of apoptosis: decrease of mitochondrial membrane potential and subsequent morphological changes. In contrast, although Hsp27 has been recently shown to inhibit apoptosis induced by various stimuli, its overexpression has no effect on apoptosis in this cell system. It should be also noticed that the apoptotic stimuli (temperature shift or hydrogen peroxide treatment) induce Hsp27, but not Bcl-2 accumulation suggesting that, in parental cells, Hsp27 might already provide some protection. However, taken together these results suggest that Hsp27, as well as Bcl-2, acts at several levels to inhibit cell death, but that their protective functions only partially overlap.

Authors+Show Affiliations

Centre de Génétique Moléculaire, UPR 9061 du CNRS, Gif-sur-Yvette cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9233769

Citation

Guénal, I, et al. "Bcl-2 and Hsp27 Act at Different Levels to Suppress Programmed Cell Death." Oncogene, vol. 15, no. 3, 1997, pp. 347-60.
Guénal I, Sidoti-de Fraisse C, Gaumer S, et al. Bcl-2 and Hsp27 act at different levels to suppress programmed cell death. Oncogene. 1997;15(3):347-60.
Guénal, I., Sidoti-de Fraisse, C., Gaumer, S., & Mignotte, B. (1997). Bcl-2 and Hsp27 act at different levels to suppress programmed cell death. Oncogene, 15(3), pp. 347-60.
Guénal I, et al. Bcl-2 and Hsp27 Act at Different Levels to Suppress Programmed Cell Death. Oncogene. 1997 Jul 17;15(3):347-60. PubMed PMID: 9233769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bcl-2 and Hsp27 act at different levels to suppress programmed cell death. AU - Guénal,I, AU - Sidoti-de Fraisse,C, AU - Gaumer,S, AU - Mignotte,B, PY - 1997/7/17/pubmed PY - 1997/7/17/medline PY - 1997/7/17/entrez SP - 347 EP - 60 JF - Oncogene JO - Oncogene VL - 15 IS - 3 N2 - Apoptosis and necrosis, two morphologically distinct forms of cell death, can be induced by common stimuli depending on the doses and the cell type. This study compares the protective effect of oncoprotein Bcl-2 and of the small stress protein Hsp27 on these two types of cell death. We use rat embryo fibroblasts conditionally immortalized by the tsA58 mutant of SV40 large T antigen as parental cells to develop cell lines carrying inducible bcl-2 or hsp27 genes. Two apoptotic stimuli were used: shift to the restrictive temperature that induced p53-mediated apoptosis and treatment with low doses of hydrogen peroxide. Necrosis was induced by high doses of hydrogen peroxide. Although Bcl-2 and Hsp27 protect these cells from necrotic death, only Bcl-2 appears capable of preventing apoptotic death. Bcl-2 protection is not mediated by a negative effect on the induction of the p53 responsive genes bax or waf1 but it slows down at least two stages of apoptosis: decrease of mitochondrial membrane potential and subsequent morphological changes. In contrast, although Hsp27 has been recently shown to inhibit apoptosis induced by various stimuli, its overexpression has no effect on apoptosis in this cell system. It should be also noticed that the apoptotic stimuli (temperature shift or hydrogen peroxide treatment) induce Hsp27, but not Bcl-2 accumulation suggesting that, in parental cells, Hsp27 might already provide some protection. However, taken together these results suggest that Hsp27, as well as Bcl-2, acts at several levels to inhibit cell death, but that their protective functions only partially overlap. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/9233769/Bcl_2_and_Hsp27_act_at_different_levels_to_suppress_programmed_cell_death_ L2 - http://dx.doi.org/10.1038/sj.onc.1201182 DB - PRIME DP - Unbound Medicine ER -