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Clodronate prevents bone loss in aged ovariectomized rats.
Calcif Tissue Int. 1997 Aug; 61(2):151-7.CT

Abstract

The purpose of this study was to investigate the ability of clodronate to prevent ovariectomy (OVX)-induced osteopenia in aged rats. Fourteen-month-old female Sprague-Dawley rats (n = 166) were randomized into six groups. One group was sacrificed at the start of the study, four groups were ovariectomized, and one group was sham-operated (Sham). The OVX rats were given subcutaneously either vehicle (veh) or clodronate at doses of 3, 7, or 25 mg/kg once a week for 3 months, and the Sham rats were given the vehicle. At all dose levels clodronate inhibited trabecular bone loss in the distal femur and in the fourth lumbar vertebral body (L4), and decreased bone resorption as evidenced by urinary deoxypyridinoline excretion. The lowest dose of clodronate preserved serum osteocalcin and endosteal bone formation of secondary spongiosa in L4 at the level of the Sham/veh group. The OVX-induced increase in periosteal bone formation of femoral diaphysis was unaffected by two smaller doses of clodronate, but was decreased to the level of Sham rats after the highest dose. After 3 mg/kg clodronate, the percentage of femoral cortical bone area and the mean relative cortical thickness were higher compared with the OVX/veh group. There was a good positive correlation between the maximum load in three-point bending of the tibia and tibial ash weight. Normal lamellar pattern of newly formed cancellous and cortical bone was found after clodronate treatment. No signs of adverse accumulation of osteoid or any deleterious effect on mechanical strength of long bones and lumbar vertebrae were found.

Authors+Show Affiliations

Leiras Oy, Biomedical Research Center, P.O. Box 415, 20101 Turku, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9236264

Citation

Kippo, K, et al. "Clodronate Prevents Bone Loss in Aged Ovariectomized Rats." Calcified Tissue International, vol. 61, no. 2, 1997, pp. 151-7.
Kippo K, Hannuniemi R, Laurén L, et al. Clodronate prevents bone loss in aged ovariectomized rats. Calcif Tissue Int. 1997;61(2):151-7.
Kippo, K., Hannuniemi, R., Laurén, L., Peng, Z., Isaksson, P., Virtamo, T., Osterman, T., Pasanen, I., Sellman, R., & Väänänen, H. K. (1997). Clodronate prevents bone loss in aged ovariectomized rats. Calcified Tissue International, 61(2), 151-7.
Kippo K, et al. Clodronate Prevents Bone Loss in Aged Ovariectomized Rats. Calcif Tissue Int. 1997;61(2):151-7. PubMed PMID: 9236264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clodronate prevents bone loss in aged ovariectomized rats. AU - Kippo,K, AU - Hannuniemi,R, AU - Laurén,L, AU - Peng,Z, AU - Isaksson,P, AU - Virtamo,T, AU - Osterman,T, AU - Pasanen,I, AU - Sellman,R, AU - Väänänen,H K, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 151 EP - 7 JF - Calcified tissue international JO - Calcif Tissue Int VL - 61 IS - 2 N2 - The purpose of this study was to investigate the ability of clodronate to prevent ovariectomy (OVX)-induced osteopenia in aged rats. Fourteen-month-old female Sprague-Dawley rats (n = 166) were randomized into six groups. One group was sacrificed at the start of the study, four groups were ovariectomized, and one group was sham-operated (Sham). The OVX rats were given subcutaneously either vehicle (veh) or clodronate at doses of 3, 7, or 25 mg/kg once a week for 3 months, and the Sham rats were given the vehicle. At all dose levels clodronate inhibited trabecular bone loss in the distal femur and in the fourth lumbar vertebral body (L4), and decreased bone resorption as evidenced by urinary deoxypyridinoline excretion. The lowest dose of clodronate preserved serum osteocalcin and endosteal bone formation of secondary spongiosa in L4 at the level of the Sham/veh group. The OVX-induced increase in periosteal bone formation of femoral diaphysis was unaffected by two smaller doses of clodronate, but was decreased to the level of Sham rats after the highest dose. After 3 mg/kg clodronate, the percentage of femoral cortical bone area and the mean relative cortical thickness were higher compared with the OVX/veh group. There was a good positive correlation between the maximum load in three-point bending of the tibia and tibial ash weight. Normal lamellar pattern of newly formed cancellous and cortical bone was found after clodronate treatment. No signs of adverse accumulation of osteoid or any deleterious effect on mechanical strength of long bones and lumbar vertebrae were found. SN - 0171-967X UR - https://www.unboundmedicine.com/medline/citation/9236264/Clodronate_prevents_bone_loss_in_aged_ovariectomized_rats_ L2 - https://dx.doi.org/10.1007/s002239900314 DB - PRIME DP - Unbound Medicine ER -