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Electrophoretic characterisation of fractions collected from gluten protein extracts subjected to size-exclusion high-performance liquid chromatography.
Electrophoresis. 1997 Jun; 18(7):1064-7.E

Abstract

The electrophoretic analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; reduced and unreduced) of fractions, collected from a size exclusion-high performance liquid chromatography (SE-HPLC) separation of gluten proteins using a column with pore size of around 400A, showed clear resolution for the seven elution ranges studied in two Australian bread wheat lines. Polymeric proteins - high molecular weight (HMW) glutenin subunits, low molecular weight (LMW) glutenin subunits, HMW albumins and some modified omega-gliadins - appeared exclusively in the region within the first peak of the chromatogram (fractions 1 to 5), the limit being a region that resolved as a small peak before the large peak of gliadins and where some omega-gliadins eluted. A larger proportion of HMW glutenin subunits and B subunits contributed to polymer formation of higher molecular weight. The polymer size decreased as the proportion of the other protein components increased.

Authors+Show Affiliations

Larroque OR
CSIRO, Division of Plant Industry, North Ryde, NSW, Australia.
Gianibelli MC
No affiliation info available
Batey IL
No affiliation info available
MacRitchie F
No affiliation info available

MeSH

Chemical FractionationChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelGlutensTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9237556

Citation

Larroque, O R., et al. "Electrophoretic Characterisation of Fractions Collected From Gluten Protein Extracts Subjected to Size-exclusion High-performance Liquid Chromatography." Electrophoresis, vol. 18, no. 7, 1997, pp. 1064-7.
Larroque OR, Gianibelli MC, Batey IL, et al. Electrophoretic characterisation of fractions collected from gluten protein extracts subjected to size-exclusion high-performance liquid chromatography. Electrophoresis. 1997;18(7):1064-7.
Larroque, O. R., Gianibelli, M. C., Batey, I. L., & MacRitchie, F. (1997). Electrophoretic characterisation of fractions collected from gluten protein extracts subjected to size-exclusion high-performance liquid chromatography. Electrophoresis, 18(7), 1064-7.
Larroque OR, et al. Electrophoretic Characterisation of Fractions Collected From Gluten Protein Extracts Subjected to Size-exclusion High-performance Liquid Chromatography. Electrophoresis. 1997;18(7):1064-7. PubMed PMID: 9237556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrophoretic characterisation of fractions collected from gluten protein extracts subjected to size-exclusion high-performance liquid chromatography. AU - Larroque,O R, AU - Gianibelli,M C, AU - Batey,I L, AU - MacRitchie,F, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 1064 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 18 IS - 7 N2 - The electrophoretic analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; reduced and unreduced) of fractions, collected from a size exclusion-high performance liquid chromatography (SE-HPLC) separation of gluten proteins using a column with pore size of around 400A, showed clear resolution for the seven elution ranges studied in two Australian bread wheat lines. Polymeric proteins - high molecular weight (HMW) glutenin subunits, low molecular weight (LMW) glutenin subunits, HMW albumins and some modified omega-gliadins - appeared exclusively in the region within the first peak of the chromatogram (fractions 1 to 5), the limit being a region that resolved as a small peak before the large peak of gliadins and where some omega-gliadins eluted. A larger proportion of HMW glutenin subunits and B subunits contributed to polymer formation of higher molecular weight. The polymer size decreased as the proportion of the other protein components increased. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9237556/Electrophoretic_characterisation_of_fractions_collected_from_gluten_protein_extracts_subjected_to_size_exclusion_high_performance_liquid_chromatography_ L2 - https://doi.org/10.1002/elps.1150180706 DB - PRIME DP - Unbound Medicine ER -