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Fast separation of underivatized carbohydrates by coelectroosmotic capillary electrophoresis.
Electrophoresis. 1997 Jun; 18(7):1142-7.E

Abstract

A method for the rapid quantitative analysis of underivatized acidic sugars, monosaccharides and disaccharides using coelectroosmotic capillary electrophoresis was developed. Indirect UV detection at 254 nm using sorbate as background electrolyte was employed for monitoring the analytes. A highly alkaline pH value of the electrolyte system was chosen in order to achieve an electrophoretic mobility of the saccharides towards the anode. A dynamic reversal of the electroosmotic flow and, by this means, a codirectional movement of the negatively charged analytes and the electroosmotic flow is accomplished by employing a polycationic surfactant (hexadimethrine bromide), which is added to the background electrolyte. To further improve the resolution of specific carbohydrates, acetone is used as organic modifier. A practical application of the developed method for the fast determination of fructose, glucose, and sucrose in various soft drinks is provided.

Authors+Show Affiliations

Zemann A
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens-University Innsbruck, Austria. andreas.zemann@uibk.ac.at
Nguyen DT
No affiliation info available
Bonn G
No affiliation info available

MeSH

AcetoneCarbohydratesDisaccharidesElectrolytesElectrophoresis, CapillaryHydrogen-Ion ConcentrationMonosaccharidesReproducibility of ResultsSensitivity and SpecificitySolventsSugar Acids

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9237570

Citation

Zemann, A, et al. "Fast Separation of Underivatized Carbohydrates By Coelectroosmotic Capillary Electrophoresis." Electrophoresis, vol. 18, no. 7, 1997, pp. 1142-7.
Zemann A, Nguyen DT, Bonn G. Fast separation of underivatized carbohydrates by coelectroosmotic capillary electrophoresis. Electrophoresis. 1997;18(7):1142-7.
Zemann, A., Nguyen, D. T., & Bonn, G. (1997). Fast separation of underivatized carbohydrates by coelectroosmotic capillary electrophoresis. Electrophoresis, 18(7), 1142-7.
Zemann A, Nguyen DT, Bonn G. Fast Separation of Underivatized Carbohydrates By Coelectroosmotic Capillary Electrophoresis. Electrophoresis. 1997;18(7):1142-7. PubMed PMID: 9237570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast separation of underivatized carbohydrates by coelectroosmotic capillary electrophoresis. AU - Zemann,A, AU - Nguyen,D T, AU - Bonn,G, PY - 1997/6/1/pubmed PY - 1997/6/1/medline PY - 1997/6/1/entrez SP - 1142 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 18 IS - 7 N2 - A method for the rapid quantitative analysis of underivatized acidic sugars, monosaccharides and disaccharides using coelectroosmotic capillary electrophoresis was developed. Indirect UV detection at 254 nm using sorbate as background electrolyte was employed for monitoring the analytes. A highly alkaline pH value of the electrolyte system was chosen in order to achieve an electrophoretic mobility of the saccharides towards the anode. A dynamic reversal of the electroosmotic flow and, by this means, a codirectional movement of the negatively charged analytes and the electroosmotic flow is accomplished by employing a polycationic surfactant (hexadimethrine bromide), which is added to the background electrolyte. To further improve the resolution of specific carbohydrates, acetone is used as organic modifier. A practical application of the developed method for the fast determination of fructose, glucose, and sucrose in various soft drinks is provided. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9237570/Fast_separation_of_underivatized_carbohydrates_by_coelectroosmotic_capillary_electrophoresis_ L2 - https://doi.org/10.1002/elps.1150180720 DB - PRIME DP - Unbound Medicine ER -