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Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats.
J Toxicol Environ Health. 1997 Aug 29; 51(6):541-55.JT

Abstract

This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development. Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient, with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed.

Authors+Show Affiliations

Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-0647, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9242227

Citation

Khanna, P, et al. "Oxidative Defense Enzyme Activity and mRNA Levels in Lenses of Diabetic Rats." Journal of Toxicology and Environmental Health, vol. 51, no. 6, 1997, pp. 541-55.
Khanna P, Wang L, Perez-Polo RJ, et al. Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats. J Toxicol Environ Health. 1997;51(6):541-55.
Khanna, P., Wang, L., Perez-Polo, R. J., & Ansari, N. H. (1997). Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats. Journal of Toxicology and Environmental Health, 51(6), 541-55.
Khanna P, et al. Oxidative Defense Enzyme Activity and mRNA Levels in Lenses of Diabetic Rats. J Toxicol Environ Health. 1997 Aug 29;51(6):541-55. PubMed PMID: 9242227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative defense enzyme activity and mRNA levels in lenses of diabetic rats. AU - Khanna,P, AU - Wang,L, AU - Perez-Polo,R J, AU - Ansari,N H, PY - 1997/8/29/pubmed PY - 1997/8/29/medline PY - 1997/8/29/entrez SP - 541 EP - 55 JF - Journal of toxicology and environmental health JO - J Toxicol Environ Health VL - 51 IS - 6 N2 - This study examines the mRNA expression and enzyme activity of oxidative defense enzymes during the course of streptozotocin-induced hyperglycemic cataract development. Diabetes was produced in 5-wk-old male Sprague-Dawley rats by administering streptozotocin ip and mRNA expression and enzyme activity were monitored on d 4, 8, 12, 16, 20, 40, 60, and 80; concomitantly, the onset and progress of cataract was followed by digital image analysis. Peak enzyme activity and mRNA expression were attained between d 20 and 40. Although catalase and glutathione peroxidase maintained high levels of mRNA expression through d 60, induction of CuZu-superoxide dismutase was transient, with the activity and mRNA levels returning to baseline values by d 40. There was a pronounced increase in aldose reductase activity, which gradually declined to basal levels by d 60; however, the mRNA levels remained unaltered. Other changes included a progressive loss of lenticular transparency, which declined to 40% of control by d 80. The role of antioxidant defense enzymes and, more interestingly, aldose reductase in combating oxidative stress in diabetic cataractogenesis is discussed. SN - 0098-4108 UR - https://www.unboundmedicine.com/medline/citation/9242227/Oxidative_defense_enzyme_activity_and_mRNA_levels_in_lenses_of_diabetic_rats_ DB - PRIME DP - Unbound Medicine ER -