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c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells.
Oncogene. 1997 Jul 10; 15(2):223-30.O

Abstract

Previous studies have shown that treatment of avian reticuloendotheliosis virus-transformed RECC-UTC4-1 (C4-1) lymphoblastoid cells with 10 microg/ml (18.8 microM) of RRR-alpha-tocopheryl succinate (vitamin E succinate, VES) for 3 days induced approximately 50% of the cells to undergo apoptosis. Elevated and prolonged expression of c-jun mRNA and protein was temporally correlated with VES-induced cell death. Data presented in this paper show that the elevated and prolonged expression of c-jun message and protein are not accounted for by enhanced stability, and show the involvement of c-Jun in VES-induced apoptosis in this lymphoblastoid cell type. C4-1 cells infected with a virus carrying a dominant, negatively acting mutant form of c-Jun, supjun-1, exhibited: (i) 71% reduction in VES-induced apoptosis, (ii) a 2.0-2.5-fold decrease in wildtype, endogenous c-Jun expression, and (iii) a 2.4-2.6-fold reduction in AP-1 binding activity. Additionally, cells co-treated with VES plus RRR-alpha-tocopherol, exhibited a 70% reduction in apoptosis, a marked reduction in c-Jun expression and a 1.6-fold reduction in AP-1 binding activity. These studies suggest that c-Jun plays a crucial role in VES-induced apoptosis in C4-1 cells, and add to our understanding of mechanisms of action involved in VES-mediated tumor cell growth inhibition.

Authors+Show Affiliations

Genetics Institute, Academy of Sciences of Czech Republic, Prague.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9244357

Citation

Qian, M, et al. "C-Jun Involvement in Vitamin E Succinate Induced Apoptosis of Reticuloendotheliosis Virus Transformed Avian Lymphoid Cells." Oncogene, vol. 15, no. 2, 1997, pp. 223-30.
Qian M, Kralova J, Yu W, et al. C-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells. Oncogene. 1997;15(2):223-30.
Qian, M., Kralova, J., Yu, W., Bose, H. R., Dvorak, M., Sanders, B. G., & Kline, K. (1997). C-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells. Oncogene, 15(2), 223-30.
Qian M, et al. C-Jun Involvement in Vitamin E Succinate Induced Apoptosis of Reticuloendotheliosis Virus Transformed Avian Lymphoid Cells. Oncogene. 1997 Jul 10;15(2):223-30. PubMed PMID: 9244357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells. AU - Qian,M, AU - Kralova,J, AU - Yu,W, AU - Bose,H R,Jr AU - Dvorak,M, AU - Sanders,B G, AU - Kline,K, PY - 1997/7/10/pubmed PY - 2001/3/28/medline PY - 1997/7/10/entrez SP - 223 EP - 30 JF - Oncogene JO - Oncogene VL - 15 IS - 2 N2 - Previous studies have shown that treatment of avian reticuloendotheliosis virus-transformed RECC-UTC4-1 (C4-1) lymphoblastoid cells with 10 microg/ml (18.8 microM) of RRR-alpha-tocopheryl succinate (vitamin E succinate, VES) for 3 days induced approximately 50% of the cells to undergo apoptosis. Elevated and prolonged expression of c-jun mRNA and protein was temporally correlated with VES-induced cell death. Data presented in this paper show that the elevated and prolonged expression of c-jun message and protein are not accounted for by enhanced stability, and show the involvement of c-Jun in VES-induced apoptosis in this lymphoblastoid cell type. C4-1 cells infected with a virus carrying a dominant, negatively acting mutant form of c-Jun, supjun-1, exhibited: (i) 71% reduction in VES-induced apoptosis, (ii) a 2.0-2.5-fold decrease in wildtype, endogenous c-Jun expression, and (iii) a 2.4-2.6-fold reduction in AP-1 binding activity. Additionally, cells co-treated with VES plus RRR-alpha-tocopherol, exhibited a 70% reduction in apoptosis, a marked reduction in c-Jun expression and a 1.6-fold reduction in AP-1 binding activity. These studies suggest that c-Jun plays a crucial role in VES-induced apoptosis in C4-1 cells, and add to our understanding of mechanisms of action involved in VES-mediated tumor cell growth inhibition. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/9244357/c_Jun_involvement_in_vitamin_E_succinate_induced_apoptosis_of_reticuloendotheliosis_virus_transformed_avian_lymphoid_cells_ DB - PRIME DP - Unbound Medicine ER -