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The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4.
Eur J Immunol. 1997 Jul; 27(7):1788-93.EJ

Abstract

In the bone marrow, progenitor (pro-) and precursor (pre-) B cells depend on close contact with stromal cells for growth and maturation. Stromal cell-derived factor 1 (SDF-1), also known as pre-B cell growth-stimulating factor, is produced by bone marrow stromal cells and was reported to act together with interleukin-7 as co-mitogen for pre-B cells. SDF-1 was recently shown to be a chemokine which is chemotactic for different types of leukocytes and acts via the chemokine receptor CXCR4. Using sorted B220+ bone marrow cells and several B cell lines characteristic for different stages of B lymphopoiesis, we now show that SDF-1 is a potent attractant for pro- and pre-B cells, but is inactive on B cells at later stages of development. In early B cell precursors, SDF-1 induced intracellular Ca2+ mobilization and in vitro migration with a potency and efficacy similar to that observed for chemokines acting on blood leukocytes. These responses were mediated via CXCR4 as they could be inhibited by an antireceptor antibody. SDF-1 is the first chemokine shown to act on early-stage B cell precursors. Mice lacking SDF-1 die perinatally and show a severe deficiency in B lymphopoiesis. We propose that SDF-1 released from the stromal cells exerts its critical hematopoietic function by selectively attracting and confining early B cell precursors within the bone marrow microenvironment that provides the necessary factors for growth and differentiation.

Authors+Show Affiliations

Theodor-Kocher Institute, University of Bern, Switzerland. dapuzzo@tki.unibe.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9247593

Citation

D'Apuzzo, M, et al. "The Chemokine SDF-1, Stromal Cell-derived Factor 1, Attracts Early Stage B Cell Precursors Via the Chemokine Receptor CXCR4." European Journal of Immunology, vol. 27, no. 7, 1997, pp. 1788-93.
D'Apuzzo M, Rolink A, Loetscher M, et al. The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4. Eur J Immunol. 1997;27(7):1788-93.
D'Apuzzo, M., Rolink, A., Loetscher, M., Hoxie, J. A., Clark-Lewis, I., Melchers, F., Baggiolini, M., & Moser, B. (1997). The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4. European Journal of Immunology, 27(7), 1788-93.
D'Apuzzo M, et al. The Chemokine SDF-1, Stromal Cell-derived Factor 1, Attracts Early Stage B Cell Precursors Via the Chemokine Receptor CXCR4. Eur J Immunol. 1997;27(7):1788-93. PubMed PMID: 9247593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4. AU - D'Apuzzo,M, AU - Rolink,A, AU - Loetscher,M, AU - Hoxie,J A, AU - Clark-Lewis,I, AU - Melchers,F, AU - Baggiolini,M, AU - Moser,B, PY - 1997/7/1/pubmed PY - 1997/7/1/medline PY - 1997/7/1/entrez SP - 1788 EP - 93 JF - European journal of immunology JO - Eur J Immunol VL - 27 IS - 7 N2 - In the bone marrow, progenitor (pro-) and precursor (pre-) B cells depend on close contact with stromal cells for growth and maturation. Stromal cell-derived factor 1 (SDF-1), also known as pre-B cell growth-stimulating factor, is produced by bone marrow stromal cells and was reported to act together with interleukin-7 as co-mitogen for pre-B cells. SDF-1 was recently shown to be a chemokine which is chemotactic for different types of leukocytes and acts via the chemokine receptor CXCR4. Using sorted B220+ bone marrow cells and several B cell lines characteristic for different stages of B lymphopoiesis, we now show that SDF-1 is a potent attractant for pro- and pre-B cells, but is inactive on B cells at later stages of development. In early B cell precursors, SDF-1 induced intracellular Ca2+ mobilization and in vitro migration with a potency and efficacy similar to that observed for chemokines acting on blood leukocytes. These responses were mediated via CXCR4 as they could be inhibited by an antireceptor antibody. SDF-1 is the first chemokine shown to act on early-stage B cell precursors. Mice lacking SDF-1 die perinatally and show a severe deficiency in B lymphopoiesis. We propose that SDF-1 released from the stromal cells exerts its critical hematopoietic function by selectively attracting and confining early B cell precursors within the bone marrow microenvironment that provides the necessary factors for growth and differentiation. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/9247593/The_chemokine_SDF_1_stromal_cell_derived_factor_1_attracts_early_stage_B_cell_precursors_via_the_chemokine_receptor_CXCR4_ L2 - https://doi.org/10.1002/eji.1830270729 DB - PRIME DP - Unbound Medicine ER -