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Separation of chiral amino acids by micellular electrokinetic chromatography with derivatized cyclodextrins.
Biomed Chromatogr. 1997 Jul-Aug; 11(4):230-5.BC

Abstract

The chiral separation of several amino acid (AA) enantiomers derivatized with naphthalene-2,3-dialdehyde (NDA) was achieved by use of cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Both neutral hydroxypropyl beta-cyclodextrin (HP-beta-CD) and charged carboxymethyl beta-cyclodextrin (CM-beta-CD) were used as buffer additives for optical resolution of derivatized amino acids. The order of elution and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl suphate, CD and amino acid system. Furthermore, the importance of SDS for the separation of a mixture of AAs, and the effect of CD and analyte concentration on the resolution will be discussed.

Authors+Show Affiliations

DeSilva K
Department of Chemistry and Pharmaceutical Chemistry, University of Kansas, Lawrence 66047, USA.
Kuwana T
No affiliation info available

MeSH

Amino AcidsCyclodextrinsElectrophoresis, CapillaryMicellesOsmolar ConcentrationStereoisomerism

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9257000

Citation

DeSilva, K, and T Kuwana. "Separation of Chiral Amino Acids By Micellular Electrokinetic Chromatography With Derivatized Cyclodextrins." Biomedical Chromatography : BMC, vol. 11, no. 4, 1997, pp. 230-5.
DeSilva K, Kuwana T. Separation of chiral amino acids by micellular electrokinetic chromatography with derivatized cyclodextrins. Biomed Chromatogr. 1997;11(4):230-5.
DeSilva, K., & Kuwana, T. (1997). Separation of chiral amino acids by micellular electrokinetic chromatography with derivatized cyclodextrins. Biomedical Chromatography : BMC, 11(4), 230-5.
DeSilva K, Kuwana T. Separation of Chiral Amino Acids By Micellular Electrokinetic Chromatography With Derivatized Cyclodextrins. Biomed Chromatogr. 1997 Jul-Aug;11(4):230-5. PubMed PMID: 9257000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of chiral amino acids by micellular electrokinetic chromatography with derivatized cyclodextrins. AU - DeSilva,K, AU - Kuwana,T, PY - 1997/7/1/pubmed PY - 2000/6/20/medline PY - 1997/7/1/entrez SP - 230 EP - 5 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 11 IS - 4 N2 - The chiral separation of several amino acid (AA) enantiomers derivatized with naphthalene-2,3-dialdehyde (NDA) was achieved by use of cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Both neutral hydroxypropyl beta-cyclodextrin (HP-beta-CD) and charged carboxymethyl beta-cyclodextrin (CM-beta-CD) were used as buffer additives for optical resolution of derivatized amino acids. The order of elution and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl suphate, CD and amino acid system. Furthermore, the importance of SDS for the separation of a mixture of AAs, and the effect of CD and analyte concentration on the resolution will be discussed. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/9257000/Separation_of_chiral_amino_acids_by_micellular_electrokinetic_chromatography_with_derivatized_cyclodextrins_ DB - PRIME DP - Unbound Medicine ER -