Separation of chiral amino acids by micellular electrokinetic chromatography with derivatized cyclodextrins.Biomed Chromatogr. 1997 Jul-Aug; 11(4):230-5.BC
The chiral separation of several amino acid (AA) enantiomers derivatized with naphthalene-2,3-dialdehyde (NDA) was achieved by use of cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Both neutral hydroxypropyl beta-cyclodextrin (HP-beta-CD) and charged carboxymethyl beta-cyclodextrin (CM-beta-CD) were used as buffer additives for optical resolution of derivatized amino acids. The order of elution and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl suphate, CD and amino acid system. Furthermore, the importance of SDS for the separation of a mixture of AAs, and the effect of CD and analyte concentration on the resolution will be discussed.