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Enhanced expression of high-affinity IgE receptor (Fc epsilon RI) alpha chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells.
J Allergy Clin Immunol. 1997 Jul; 100(1):78-86.JA

Abstract

BACKGROUND

IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (Fc epsilon RI) is involved in the pathogenesis of allergen-induced immediate and late responses.

OBJECTIVE

We investigated the expression and cellular distribution of Fc epsilon RI in the nasal mucosa after allergen challenge in patients with summer hay fever.

METHODS

Fourteen grass pollen-sensitive patients and seven normal control subjects underwent nasal challenge with grass pollen and allergen diluent in random order separated by 2 weeks. Nasal airway caliber was monitored by acoustic rhinometry, and nasal biopsy was performed at 6 hours. Messenger RNA for Fc epsilon RI was determined by using reverse-transcription polymerase chain reaction, and Fc epsilon RI protein expression was determined by immunohistology with a mouse monoclonal antibody (22E7) and a rabbit polyclonal antibody (997) directed against the alpha subunit. Co-localization of Fc epsilon RI receptors was performed by using double-immunostaining methods.

RESULTS

In atopic subjects, there was a significant early decrease in nasal airway caliber, which extended up to 6 hours after allergen challenge. Fc epsilon RI mRNA levels were elevated at 6 hours (p = 0.03). Cells expressing Fc epsilon RI protein were increased in patients with atopic rhinitis compared with normal control subjects (p = 0.03). Further increases in Fc epsilon RI+ cells were observed after allergen challenge only in the atopic group (p = 0.02). Double immunohistochemistry revealed that the majority of Fc epsilon RI+ cells were mast cells (64%), followed by macrophages (20%), eosinophils (4%), and dendritic cells (2%), with 10% Fc epsilon RI+ cells being unidentified.

CONCLUSIONS

Our results demonstrate increased Fc epsilon RI expression during allergen-induced rhinitis and highlight a potential target for treatment.

Authors+Show Affiliations

Upper Respiratory Medicine, Imperial College of Medicine, National Heart and Lung Institute, London, England.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

9257791

Citation

Rajakulasingam, K, et al. "Enhanced Expression of High-affinity IgE Receptor (Fc Epsilon RI) Alpha Chain in Human Allergen-induced Rhinitis With Co-localization to Mast Cells, Macrophages, Eosinophils, and Dendritic Cells." The Journal of Allergy and Clinical Immunology, vol. 100, no. 1, 1997, pp. 78-86.
Rajakulasingam K, Durham SR, O'Brien F, et al. Enhanced expression of high-affinity IgE receptor (Fc epsilon RI) alpha chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells. J Allergy Clin Immunol. 1997;100(1):78-86.
Rajakulasingam, K., Durham, S. R., O'Brien, F., Humbert, M., Barata, L. T., Reece, L., Kay, A. B., & Grant, J. A. (1997). Enhanced expression of high-affinity IgE receptor (Fc epsilon RI) alpha chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells. The Journal of Allergy and Clinical Immunology, 100(1), 78-86.
Rajakulasingam K, et al. Enhanced Expression of High-affinity IgE Receptor (Fc Epsilon RI) Alpha Chain in Human Allergen-induced Rhinitis With Co-localization to Mast Cells, Macrophages, Eosinophils, and Dendritic Cells. J Allergy Clin Immunol. 1997;100(1):78-86. PubMed PMID: 9257791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced expression of high-affinity IgE receptor (Fc epsilon RI) alpha chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells. AU - Rajakulasingam,K, AU - Durham,S R, AU - O'Brien,F, AU - Humbert,M, AU - Barata,L T, AU - Reece,L, AU - Kay,A B, AU - Grant,J A, PY - 1997/7/1/pubmed PY - 1997/7/1/medline PY - 1997/7/1/entrez SP - 78 EP - 86 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 100 IS - 1 N2 - BACKGROUND: IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (Fc epsilon RI) is involved in the pathogenesis of allergen-induced immediate and late responses. OBJECTIVE: We investigated the expression and cellular distribution of Fc epsilon RI in the nasal mucosa after allergen challenge in patients with summer hay fever. METHODS: Fourteen grass pollen-sensitive patients and seven normal control subjects underwent nasal challenge with grass pollen and allergen diluent in random order separated by 2 weeks. Nasal airway caliber was monitored by acoustic rhinometry, and nasal biopsy was performed at 6 hours. Messenger RNA for Fc epsilon RI was determined by using reverse-transcription polymerase chain reaction, and Fc epsilon RI protein expression was determined by immunohistology with a mouse monoclonal antibody (22E7) and a rabbit polyclonal antibody (997) directed against the alpha subunit. Co-localization of Fc epsilon RI receptors was performed by using double-immunostaining methods. RESULTS: In atopic subjects, there was a significant early decrease in nasal airway caliber, which extended up to 6 hours after allergen challenge. Fc epsilon RI mRNA levels were elevated at 6 hours (p = 0.03). Cells expressing Fc epsilon RI protein were increased in patients with atopic rhinitis compared with normal control subjects (p = 0.03). Further increases in Fc epsilon RI+ cells were observed after allergen challenge only in the atopic group (p = 0.02). Double immunohistochemistry revealed that the majority of Fc epsilon RI+ cells were mast cells (64%), followed by macrophages (20%), eosinophils (4%), and dendritic cells (2%), with 10% Fc epsilon RI+ cells being unidentified. CONCLUSIONS: Our results demonstrate increased Fc epsilon RI expression during allergen-induced rhinitis and highlight a potential target for treatment. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/9257791/Enhanced_expression_of_high_affinity_IgE_receptor__Fc_epsilon_RI__alpha_chain_in_human_allergen_induced_rhinitis_with_co_localization_to_mast_cells_macrophages_eosinophils_and_dendritic_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674997001735 DB - PRIME DP - Unbound Medicine ER -