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Acquired resistance to experimental autoimmune encephalomyelitis is independent of V beta usage.
Cell Immunol. 1997 Jul 10; 179(1):55-65.CI

Abstract

In Lewis rats, activated encephalitogenic T-helper cells elicit a single bout of experimental autoimmune encephalomyelitis (EAE). Recovery from EAE is marked by reduced susceptibility to disease reinduction. The purpose of this study was to determine whether a dominant expression of V beta gene segments by encephalitogenic T cells was required for development of recovery-associated resistance. Several polyclonal and monoclonal T cell lines were derived from Lewis rats sensitized with R72-86, a synthetic peptide representing the 72- to 86-amino-acid sequence of rat myelin basic protein (RMBP). The results revealed broad heterogeneity among encephalitogenic T cells specific for R72-86 in regard to V beta expression and CDR3 sequence. Encephalitogenic clones exclusively bearing either V beta 4 or V beta 10 TCR or polyclonal T cells bearing heterogeneous TCR transferred EAE to recipient rats and elicited resistance to EAE as revealed by subsequent challenge with guinea pig (GP)MBP in complete Freund's adjuvant (CFA). Nonpathogenic V beta 3+ and V beta 8.6+ clones specific for the 68-86 and 55-66 regions of MBP, respectively, did not elicit effective protection from EAE. These data indicate that induction of postrecovery resistance to EAE does not depend upon a particular V beta usage.

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Authors+Show Affiliations

Johnson BD
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina, USA.
Nardella JP
No affiliation info available
McConnell TJ
No affiliation info available
Mannie MD
No affiliation info available

MeSH

Adoptive TransferAmino Acid SequenceAnimalsBase SequenceDNAEncephalomyelitis, Autoimmune, ExperimentalGuinea PigsImmunity, InnateMolecular Sequence DataMyelin Basic ProteinRatsRats, Inbred LewReceptors, Antigen, T-Cell, alpha-betaT-Lymphocytes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9259772

Citation

Johnson, B D., et al. "Acquired Resistance to Experimental Autoimmune Encephalomyelitis Is Independent of V Beta Usage." Cellular Immunology, vol. 179, no. 1, 1997, pp. 55-65.
Johnson BD, Nardella JP, McConnell TJ, et al. Acquired resistance to experimental autoimmune encephalomyelitis is independent of V beta usage. Cell Immunol. 1997;179(1):55-65.
Johnson, B. D., Nardella, J. P., McConnell, T. J., & Mannie, M. D. (1997). Acquired resistance to experimental autoimmune encephalomyelitis is independent of V beta usage. Cellular Immunology, 179(1), 55-65.
Johnson BD, et al. Acquired Resistance to Experimental Autoimmune Encephalomyelitis Is Independent of V Beta Usage. Cell Immunol. 1997 Jul 10;179(1):55-65. PubMed PMID: 9259772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acquired resistance to experimental autoimmune encephalomyelitis is independent of V beta usage. AU - Johnson,B D, AU - Nardella,J P, AU - McConnell,T J, AU - Mannie,M D, PY - 1997/7/10/pubmed PY - 1997/7/10/medline PY - 1997/7/10/entrez SP - 55 EP - 65 JF - Cellular immunology JO - Cell Immunol VL - 179 IS - 1 N2 - In Lewis rats, activated encephalitogenic T-helper cells elicit a single bout of experimental autoimmune encephalomyelitis (EAE). Recovery from EAE is marked by reduced susceptibility to disease reinduction. The purpose of this study was to determine whether a dominant expression of V beta gene segments by encephalitogenic T cells was required for development of recovery-associated resistance. Several polyclonal and monoclonal T cell lines were derived from Lewis rats sensitized with R72-86, a synthetic peptide representing the 72- to 86-amino-acid sequence of rat myelin basic protein (RMBP). The results revealed broad heterogeneity among encephalitogenic T cells specific for R72-86 in regard to V beta expression and CDR3 sequence. Encephalitogenic clones exclusively bearing either V beta 4 or V beta 10 TCR or polyclonal T cells bearing heterogeneous TCR transferred EAE to recipient rats and elicited resistance to EAE as revealed by subsequent challenge with guinea pig (GP)MBP in complete Freund's adjuvant (CFA). Nonpathogenic V beta 3+ and V beta 8.6+ clones specific for the 68-86 and 55-66 regions of MBP, respectively, did not elicit effective protection from EAE. These data indicate that induction of postrecovery resistance to EAE does not depend upon a particular V beta usage. SN - 0008-8749 UR - https://www.unboundmedicine.com/medline/citation/9259772/Acquired_resistance_to_experimental_autoimmune_encephalomyelitis_is_independent_of_V_beta_usage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-8749(97)91143-6 DB - PRIME DP - Unbound Medicine ER -
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