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Effect of a two-year supplementation with low doses of antioxidant vitamins and/or minerals in elderly subjects on levels of nutrients and antioxidant defense parameters.
J Am Coll Nutr 1997; 16(4):357-65JA

Abstract

BACKGROUND

Eighty-one elderly hospitalized subjects (> 65 years) were recruited for a double-blind placebo-controlled study to examine low dose supplementation of antioxidant vitamins and minerals on biological and functional parameters of free radical metabolism. Subjects were randomly assigned to one of the four treatment groups, daily receiving for 2 years: placebo group; mineral group: 20 mg zinc, 100 micrograms selenium; vitamin group: 120 mg vitamin C (Vit C), 6 mg beta-carotene (beta CA), 15 mg vitamin E (Vit E); mineral and vitamin group: Zn 20 mg, Se 100 micrograms, Vit C 120 mg, beta CA 6 mg, Vit E 15 mg.

RESULTS

Fifty-seven subjects completed the study. A large frequency of Vit C, Zn and Se deficiencies were observed at baseline. As early as 6 months of treatment, a significant increase in vitamin and mineral serum levels was observed in the corresponding groups. The increases ranged from 1.1-4.0 fold depending on the nutrient. Antioxidant defense, studied in vitro with a test using red blood cells in presence of 2,2'-azo-bis (2-amidinopropane) by hydrochloride, showed an increase of cell resistance in patients receiving vitamins (p = 0.002); it was positively correlated with serum Vit C (p < 0.0001), alpha-tocopherol/cholesterol (p = 0.06), beta CA (p = 0.0014), serum Cu and Se (p < 0.05). Moreover, red blood cell antioxidant defense was reduced in elderly compared with young control subjects (50% hemolysis time: 69 +/- 14 mn and 109 +/- 12 mn, respectively). Erythrocyte glutathione peroxidase activity was enhanced in groups receiving minerals, whereas no significant change was observed for other indicators of oxidative stress (erythrocyte superoxide dismutase activity, thiobarbituric acid-reactive substances, total glutathione, reduced and oxidized forms).

DISCUSSION

Our results provide experimental evidence that a low dose supplementation with vitamins and minerals was able to normalize biological nutrient status as early as 6 months of treatment. In addition, our data indicate that antioxidant defense in elderly subjects was improved with low doses of vit C, vit E and beta CA as studied by means of a functional test utilizing red blood cells challenged in vitro with free radicals.

Authors+Show Affiliations

Laboratoire de Biochimie des Lipoprotéines, Université de Bourgogne, Digon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9263186

Citation

Girodon, F, et al. "Effect of a Two-year Supplementation With Low Doses of Antioxidant Vitamins And/or Minerals in Elderly Subjects On Levels of Nutrients and Antioxidant Defense Parameters." Journal of the American College of Nutrition, vol. 16, no. 4, 1997, pp. 357-65.
Girodon F, Blache D, Monget AL, et al. Effect of a two-year supplementation with low doses of antioxidant vitamins and/or minerals in elderly subjects on levels of nutrients and antioxidant defense parameters. J Am Coll Nutr. 1997;16(4):357-65.
Girodon, F., Blache, D., Monget, A. L., Lombart, M., Brunet-Lecompte, P., Arnaud, J., ... Galan, P. (1997). Effect of a two-year supplementation with low doses of antioxidant vitamins and/or minerals in elderly subjects on levels of nutrients and antioxidant defense parameters. Journal of the American College of Nutrition, 16(4), pp. 357-65.
Girodon F, et al. Effect of a Two-year Supplementation With Low Doses of Antioxidant Vitamins And/or Minerals in Elderly Subjects On Levels of Nutrients and Antioxidant Defense Parameters. J Am Coll Nutr. 1997;16(4):357-65. PubMed PMID: 9263186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of a two-year supplementation with low doses of antioxidant vitamins and/or minerals in elderly subjects on levels of nutrients and antioxidant defense parameters. AU - Girodon,F, AU - Blache,D, AU - Monget,A L, AU - Lombart,M, AU - Brunet-Lecompte,P, AU - Arnaud,J, AU - Richard,M J, AU - Galan,P, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 357 EP - 65 JF - Journal of the American College of Nutrition JO - J Am Coll Nutr VL - 16 IS - 4 N2 - BACKGROUND: Eighty-one elderly hospitalized subjects (> 65 years) were recruited for a double-blind placebo-controlled study to examine low dose supplementation of antioxidant vitamins and minerals on biological and functional parameters of free radical metabolism. Subjects were randomly assigned to one of the four treatment groups, daily receiving for 2 years: placebo group; mineral group: 20 mg zinc, 100 micrograms selenium; vitamin group: 120 mg vitamin C (Vit C), 6 mg beta-carotene (beta CA), 15 mg vitamin E (Vit E); mineral and vitamin group: Zn 20 mg, Se 100 micrograms, Vit C 120 mg, beta CA 6 mg, Vit E 15 mg. RESULTS: Fifty-seven subjects completed the study. A large frequency of Vit C, Zn and Se deficiencies were observed at baseline. As early as 6 months of treatment, a significant increase in vitamin and mineral serum levels was observed in the corresponding groups. The increases ranged from 1.1-4.0 fold depending on the nutrient. Antioxidant defense, studied in vitro with a test using red blood cells in presence of 2,2'-azo-bis (2-amidinopropane) by hydrochloride, showed an increase of cell resistance in patients receiving vitamins (p = 0.002); it was positively correlated with serum Vit C (p < 0.0001), alpha-tocopherol/cholesterol (p = 0.06), beta CA (p = 0.0014), serum Cu and Se (p < 0.05). Moreover, red blood cell antioxidant defense was reduced in elderly compared with young control subjects (50% hemolysis time: 69 +/- 14 mn and 109 +/- 12 mn, respectively). Erythrocyte glutathione peroxidase activity was enhanced in groups receiving minerals, whereas no significant change was observed for other indicators of oxidative stress (erythrocyte superoxide dismutase activity, thiobarbituric acid-reactive substances, total glutathione, reduced and oxidized forms). DISCUSSION: Our results provide experimental evidence that a low dose supplementation with vitamins and minerals was able to normalize biological nutrient status as early as 6 months of treatment. In addition, our data indicate that antioxidant defense in elderly subjects was improved with low doses of vit C, vit E and beta CA as studied by means of a functional test utilizing red blood cells challenged in vitro with free radicals. SN - 0731-5724 UR - https://www.unboundmedicine.com/medline/citation/9263186/Effect_of_a_two_year_supplementation_with_low_doses_of_antioxidant_vitamins_and/or_minerals_in_elderly_subjects_on_levels_of_nutrients_and_antioxidant_defense_parameters_ L2 - http://www.tandfonline.com/doi/full/10.1080/07315724.1997.10718698 DB - PRIME DP - Unbound Medicine ER -