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Cytochemical localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in the facial nerve of reserpinized guinea pigs.
J Histochem Cytochem. 1997 Aug; 45(8):1129-35.JH

Abstract

Ion-transporting Na,K-ATPase plays an essential role in nerve conduction. To clarify the cytochemical effects of reserpine on transport Na,K-ATPase activity, the localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase (K-NPPase) activity was investigated in the facial nerves of normal and reserpinized guinea pigs using a cerium-based method. In the normal facial nerve, the reaction product of K-NPPase activity was observed on the internodal axolemma and Schmidt-Lanterman incisures. In the Ranvier nodes, enzyme activity was localized to the paranodal and nodal axolemma. In the reserpinized nerves, reaction product was detectable on the nodal axolemma but was undetectable on the other parts of the axolemma. Nodal K-NPPase was not affected by reserpine treatment. Therefore, the transport Na,K-ATPase on the nodal axolemma might differ from that on the other parts of the axolemma. Allowing reserpinized animals to survive. Two different ouabain-sensitive K-NPPase reactivities, "reserpine-sensitive" and "reserpine-resistant," might be present in the facial nerve of guinea pigs.

Authors+Show Affiliations

Department of Otolaryngology, Hyogo College of Medicine, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9267473

Citation

Kanoh, N. "Cytochemical Localization of Ouabain-sensitive, K(+)-dependent P-nitrophenylphosphatase Activity in the Facial Nerve of Reserpinized Guinea Pigs." The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol. 45, no. 8, 1997, pp. 1129-35.
Kanoh N. Cytochemical localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in the facial nerve of reserpinized guinea pigs. J Histochem Cytochem. 1997;45(8):1129-35.
Kanoh, N. (1997). Cytochemical localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in the facial nerve of reserpinized guinea pigs. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 45(8), 1129-35.
Kanoh N. Cytochemical Localization of Ouabain-sensitive, K(+)-dependent P-nitrophenylphosphatase Activity in the Facial Nerve of Reserpinized Guinea Pigs. J Histochem Cytochem. 1997;45(8):1129-35. PubMed PMID: 9267473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochemical localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in the facial nerve of reserpinized guinea pigs. A1 - Kanoh,N, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 1129 EP - 35 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JO - J Histochem Cytochem VL - 45 IS - 8 N2 - Ion-transporting Na,K-ATPase plays an essential role in nerve conduction. To clarify the cytochemical effects of reserpine on transport Na,K-ATPase activity, the localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase (K-NPPase) activity was investigated in the facial nerves of normal and reserpinized guinea pigs using a cerium-based method. In the normal facial nerve, the reaction product of K-NPPase activity was observed on the internodal axolemma and Schmidt-Lanterman incisures. In the Ranvier nodes, enzyme activity was localized to the paranodal and nodal axolemma. In the reserpinized nerves, reaction product was detectable on the nodal axolemma but was undetectable on the other parts of the axolemma. Nodal K-NPPase was not affected by reserpine treatment. Therefore, the transport Na,K-ATPase on the nodal axolemma might differ from that on the other parts of the axolemma. Allowing reserpinized animals to survive. Two different ouabain-sensitive K-NPPase reactivities, "reserpine-sensitive" and "reserpine-resistant," might be present in the facial nerve of guinea pigs. SN - 0022-1554 UR - https://www.unboundmedicine.com/medline/citation/9267473/Cytochemical_localization_of_ouabain_sensitive_K_+__dependent_p_nitrophenylphosphatase_activity_in_the_facial_nerve_of_reserpinized_guinea_pigs_ L2 - https://journals.sagepub.com/doi/10.1177/002215549704500810?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -