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Ontogenic changes in proglucagon mRNA in BB diabetes prone and normal rats weaned onto a chow diet.
Diabetologia 1997; 40(8):871-8D

Abstract

Weaning onto chow diets causes the highest incidence of diabetes in the BB rat. Changes in gut development and absorption of nutrients in the diabetes prone rat and the subsequent effect on pancreatic function may play a role in the ultimate development of the disease. BB diabetes prone (dp) and BB normal (n) dams were fed chow diets. Pups were killed at various ages ranging from 7 to 30 days. BBdp rats had higher small intestine and colon weights expressed per body weight at all ages (p < 0.0001). RNA content (mg/g) in the jejunum, ileum and colon was higher in the BBdp rats beginning at the critical period at 21 days and maintained at 24 days and 30 days (p < 0.0001). Proglucagon message decreased with age in both BBdp and BBn animals (p < 0.0001). Levels of proglucagon mRNA were higher in BBdp compared to BBn animals only in the ileum at 10 days (p < 0.01). Adjusting for total ileal and colonic RNA content resulted in BBdp animals having higher total colonic proglucagon mRNA at 21, 24 and 30 days (p < 0.0001). Plasma GLP-1(7-36) amide was more than doubled in BBdp compared to BBn animals (p < 0.0005) at 30 days. Expressing sodium-dependent D-glucose co-transporter (SGLT-1), GLUT2 and GLUT5 mRNA per total jejunal RNA shows increased transporter mRNA in BBdp compared to BBn rats at weaning (21 days) (p < 0.05). Radical differences exist between BBdp and BBn animals at 'critical periods' in both proglucagon and glucose transporter gene expression. These differences may help explain altered growth and diseases incidence between these two strains.

Authors+Show Affiliations

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9267980

Citation

Reimer, R A., et al. "Ontogenic Changes in Proglucagon mRNA in BB Diabetes Prone and Normal Rats Weaned Onto a Chow Diet." Diabetologia, vol. 40, no. 8, 1997, pp. 871-8.
Reimer RA, Field CJ, McBurney MI. Ontogenic changes in proglucagon mRNA in BB diabetes prone and normal rats weaned onto a chow diet. Diabetologia. 1997;40(8):871-8.
Reimer, R. A., Field, C. J., & McBurney, M. I. (1997). Ontogenic changes in proglucagon mRNA in BB diabetes prone and normal rats weaned onto a chow diet. Diabetologia, 40(8), pp. 871-8.
Reimer RA, Field CJ, McBurney MI. Ontogenic Changes in Proglucagon mRNA in BB Diabetes Prone and Normal Rats Weaned Onto a Chow Diet. Diabetologia. 1997;40(8):871-8. PubMed PMID: 9267980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ontogenic changes in proglucagon mRNA in BB diabetes prone and normal rats weaned onto a chow diet. AU - Reimer,R A, AU - Field,C J, AU - McBurney,M I, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 871 EP - 8 JF - Diabetologia JO - Diabetologia VL - 40 IS - 8 N2 - Weaning onto chow diets causes the highest incidence of diabetes in the BB rat. Changes in gut development and absorption of nutrients in the diabetes prone rat and the subsequent effect on pancreatic function may play a role in the ultimate development of the disease. BB diabetes prone (dp) and BB normal (n) dams were fed chow diets. Pups were killed at various ages ranging from 7 to 30 days. BBdp rats had higher small intestine and colon weights expressed per body weight at all ages (p < 0.0001). RNA content (mg/g) in the jejunum, ileum and colon was higher in the BBdp rats beginning at the critical period at 21 days and maintained at 24 days and 30 days (p < 0.0001). Proglucagon message decreased with age in both BBdp and BBn animals (p < 0.0001). Levels of proglucagon mRNA were higher in BBdp compared to BBn animals only in the ileum at 10 days (p < 0.01). Adjusting for total ileal and colonic RNA content resulted in BBdp animals having higher total colonic proglucagon mRNA at 21, 24 and 30 days (p < 0.0001). Plasma GLP-1(7-36) amide was more than doubled in BBdp compared to BBn animals (p < 0.0005) at 30 days. Expressing sodium-dependent D-glucose co-transporter (SGLT-1), GLUT2 and GLUT5 mRNA per total jejunal RNA shows increased transporter mRNA in BBdp compared to BBn rats at weaning (21 days) (p < 0.05). Radical differences exist between BBdp and BBn animals at 'critical periods' in both proglucagon and glucose transporter gene expression. These differences may help explain altered growth and diseases incidence between these two strains. SN - 0012-186X UR - https://www.unboundmedicine.com/medline/citation/9267980/Ontogenic_changes_in_proglucagon_mRNA_in_BB_diabetes_prone_and_normal_rats_weaned_onto_a_chow_diet_ L2 - https://dx.doi.org/10.1007/s001250050762 DB - PRIME DP - Unbound Medicine ER -