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Stationary-phase gene expression in Saccharomyces cerevisiae during wine fermentation.
Yeast. 1997 Aug; 13(10):903-15.Y

Abstract

Genetic engineering of wine yeast strains requires the identification of gene promoters specifically activated under wine processing conditions. In this study, transcriptional activation of specific genes was followed during the time course of wine fermentation by quantifying mRNA levels in a haploid wine strain of Saccharomyces cerevisiae grown on synthetic or natural winery musts. Northern analyses were performed using radioactive probes from 19 genes previously described as being expressed under laboratory growth conditions or on molasses in S. cerevisiae during the stationary phase and/or under nitrogen starvation. Nine genes, including members of the HSP family, showed a transition-phase induction profile. For three of them, mRNA transcripts could be detected until the end of the fermentation. Expression of one of these genes, HSP30, was further studied using a HSP30::lacZ fusion on both multicopy and monocopy expression vectors. The production of beta-galactosidase by recombinant cells was measured during cell growth and fermentation on synthetic and natural winery musts. We showed that the HSP30 promoter can induce high gene expression during late stationary phase and remains active until the end of the wine fermentation process. Similar expression profiles were obtained on five natural winery musts.

Authors+Show Affiliations

Laboratoire de Microbiologie et Technologie des Fermentations, INRA-IPV, Montpellier, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9271106

Citation

Riou, C, et al. "Stationary-phase Gene Expression in Saccharomyces Cerevisiae During Wine Fermentation." Yeast (Chichester, England), vol. 13, no. 10, 1997, pp. 903-15.
Riou C, Nicaud JM, Barre P, et al. Stationary-phase gene expression in Saccharomyces cerevisiae during wine fermentation. Yeast. 1997;13(10):903-15.
Riou, C., Nicaud, J. M., Barre, P., & Gaillardin, C. (1997). Stationary-phase gene expression in Saccharomyces cerevisiae during wine fermentation. Yeast (Chichester, England), 13(10), 903-15.
Riou C, et al. Stationary-phase Gene Expression in Saccharomyces Cerevisiae During Wine Fermentation. Yeast. 1997;13(10):903-15. PubMed PMID: 9271106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stationary-phase gene expression in Saccharomyces cerevisiae during wine fermentation. AU - Riou,C, AU - Nicaud,J M, AU - Barre,P, AU - Gaillardin,C, PY - 1997/8/1/pubmed PY - 2000/6/20/medline PY - 1997/8/1/entrez SP - 903 EP - 15 JF - Yeast (Chichester, England) JO - Yeast VL - 13 IS - 10 N2 - Genetic engineering of wine yeast strains requires the identification of gene promoters specifically activated under wine processing conditions. In this study, transcriptional activation of specific genes was followed during the time course of wine fermentation by quantifying mRNA levels in a haploid wine strain of Saccharomyces cerevisiae grown on synthetic or natural winery musts. Northern analyses were performed using radioactive probes from 19 genes previously described as being expressed under laboratory growth conditions or on molasses in S. cerevisiae during the stationary phase and/or under nitrogen starvation. Nine genes, including members of the HSP family, showed a transition-phase induction profile. For three of them, mRNA transcripts could be detected until the end of the fermentation. Expression of one of these genes, HSP30, was further studied using a HSP30::lacZ fusion on both multicopy and monocopy expression vectors. The production of beta-galactosidase by recombinant cells was measured during cell growth and fermentation on synthetic and natural winery musts. We showed that the HSP30 promoter can induce high gene expression during late stationary phase and remains active until the end of the wine fermentation process. Similar expression profiles were obtained on five natural winery musts. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/9271106/Stationary_phase_gene_expression_in_Saccharomyces_cerevisiae_during_wine_fermentation_ DB - PRIME DP - Unbound Medicine ER -