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Relationship between state of a thermosensitive matrix and the activity of urease immobilized in it.

Abstract

The effect of temperature on kinetic and equilibrium parameters of urea hydrolysis catalyzed with urease immobilized into a thermosensitive poly-N-isopropylacrylamide gel was studied. The temperature behavior of the gel-urease system is different from similar systems. After a decrease in the enzyme activity above the critical temperature, the maximal rate of the enzymatic reaction and gel swelling ratio begin to increase. Urea hydrolysis catalyzed with immobilized urease and shrinking-swelling of the thermosensitive urease-containing gel depend on each other. Under collapse, gel swelling increases due to the enzymatic reaction. The rate of the enzymatic reaction no longer follows Michaelis-Menten kinetics, and the dependence of the reaction rate on substrate concentration becomes more complicated.

Authors

, , ,

Source

Biochemistry. Biokhimii͡a 62:4 1997 Apr pg 371-6

MeSH

Acrylamides
Enzymes, Immobilized
Fabaceae
Gels
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Plants, Medicinal
Temperature
Urea
Urease

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9275275

Citation

TY - JOUR T1 - Relationship between state of a thermosensitive matrix and the activity of urease immobilized in it. AU - Kukhtin,A V, AU - Eremeev,N L, AU - Belyaeva,E A, AU - Kazanskaya,N F, PY - 1997/4/1/pubmed PY - 1997/4/1/medline PY - 1997/4/1/entrez SP - 371 EP - 6 JF - Biochemistry. Biokhimii͡a JO - Biochemistry Mosc. VL - 62 IS - 4 N2 - The effect of temperature on kinetic and equilibrium parameters of urea hydrolysis catalyzed with urease immobilized into a thermosensitive poly-N-isopropylacrylamide gel was studied. The temperature behavior of the gel-urease system is different from similar systems. After a decrease in the enzyme activity above the critical temperature, the maximal rate of the enzymatic reaction and gel swelling ratio begin to increase. Urea hydrolysis catalyzed with immobilized urease and shrinking-swelling of the thermosensitive urease-containing gel depend on each other. Under collapse, gel swelling increases due to the enzymatic reaction. The rate of the enzymatic reaction no longer follows Michaelis-Menten kinetics, and the dependence of the reaction rate on substrate concentration becomes more complicated. SN - 0006-2979 UR - https://www.unboundmedicine.com/medline/citation/9275275/Relationship_between_state_of_a_thermosensitive_matrix_and_the_activity_of_urease_immobilized_in_it_ L2 - http://toxnet.nlm.nih.gov/cgi-bin/sis/search/r?dbs+hsdb:@term+@rn+57-13-6 ER -