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Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen.
J Clin Microbiol. 1997 Sep; 35(9):2252-5.JC

Abstract

The purpose of this study was to compare the conventional radioimmunoassay (RIA) to an enzyme-linked immunoassay (EIA) for the measurement of Histoplasma antigen in banked urine specimens. A correlation between the two methods would allow the EIA to be used as a nonradioactive alternative to the established 125I RIA. The study used stored urine from patients diagnosed with histoplasmosis during an outbreak in Indianapolis which began in 1988. Control specimens from healthy adults, patients with other fungal infections, urinary tract infections, or nonfungal pneumonia were also tested. Both the RIA and EIA were run concurrently. The RIA system measured antigen levels of 0.4 to 27.0 RIA units, while the EIA measured antigen levels of 0.6 to 20.1 units. Both the EIA and RIA detected measurable antigen levels in urine from 50 of 56 patients (89%) with disseminated disease and 11 of 30 patients (37%) with self-limiting disease. One of 96 control specimens, from a patient with paracoccidioidomycosis, was positive with both systems. Antigen levels measured by EIA correlated well with those measured by the established RIA method (correlation coefficient, 0.974). The EIA is an acceptable alternative to the RIA for measuring Histoplasma antigen levels in urine specimens.

Authors+Show Affiliations

Department of Medicine, Indiana University School of Medicine, Indianapolis 46262, USA. MDURKIN@MDEP.IUPUI.EDUNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9276396

Citation

Durkin, M M., et al. "Comparison of Radioimmunoassay and Enzyme-linked Immunoassay Methods for Detection of Histoplasma Capsulatum Var. Capsulatum Antigen." Journal of Clinical Microbiology, vol. 35, no. 9, 1997, pp. 2252-5.
Durkin MM, Connolly PA, Wheat LJ. Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen. J Clin Microbiol. 1997;35(9):2252-5.
Durkin, M. M., Connolly, P. A., & Wheat, L. J. (1997). Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen. Journal of Clinical Microbiology, 35(9), 2252-5.
Durkin MM, Connolly PA, Wheat LJ. Comparison of Radioimmunoassay and Enzyme-linked Immunoassay Methods for Detection of Histoplasma Capsulatum Var. Capsulatum Antigen. J Clin Microbiol. 1997;35(9):2252-5. PubMed PMID: 9276396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen. AU - Durkin,M M, AU - Connolly,P A, AU - Wheat,L J, PY - 1997/9/1/pubmed PY - 1997/9/1/medline PY - 1997/9/1/entrez SP - 2252 EP - 5 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 35 IS - 9 N2 - The purpose of this study was to compare the conventional radioimmunoassay (RIA) to an enzyme-linked immunoassay (EIA) for the measurement of Histoplasma antigen in banked urine specimens. A correlation between the two methods would allow the EIA to be used as a nonradioactive alternative to the established 125I RIA. The study used stored urine from patients diagnosed with histoplasmosis during an outbreak in Indianapolis which began in 1988. Control specimens from healthy adults, patients with other fungal infections, urinary tract infections, or nonfungal pneumonia were also tested. Both the RIA and EIA were run concurrently. The RIA system measured antigen levels of 0.4 to 27.0 RIA units, while the EIA measured antigen levels of 0.6 to 20.1 units. Both the EIA and RIA detected measurable antigen levels in urine from 50 of 56 patients (89%) with disseminated disease and 11 of 30 patients (37%) with self-limiting disease. One of 96 control specimens, from a patient with paracoccidioidomycosis, was positive with both systems. Antigen levels measured by EIA correlated well with those measured by the established RIA method (correlation coefficient, 0.974). The EIA is an acceptable alternative to the RIA for measuring Histoplasma antigen levels in urine specimens. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/9276396/Comparison_of_radioimmunoassay_and_enzyme_linked_immunoassay_methods_for_detection_of_Histoplasma_capsulatum_var__capsulatum_antigen_ L2 - https://journals.asm.org/doi/10.1128/jcm.35.9.2252-2255.1997?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -