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Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats.
J Hypertens. 1997 Aug; 15(8):863-9.JH

Abstract

OBJECTIVE

The purpose of the present study was to investigate liver microsomal membrane fluidity simultaneously with membrane fatty acid composition and desaturase activities in spontaneously hypertensive rats (SHR).

DESIGN AND METHODS

The membrane fluidity was determined, after electron spin resonance (ESR) measurement, in SHR compared with normotensive Wistar-Kyoto (WKY) rats, by calculating the order parameter S from ESR spectra of 5-nitroxide stearate and 10-nitroxide stearate, used as spin-labelled fatty acids. Desaturase activities were measured by incubating SHR and WKY rat liver microsomes with [14C]-radiolabeled fatty acids as substrates for desaturation reactions. The fatty acid composition of liver microsomal membranes was determined by gas-liquid chromatography.

RESULTS

Whereas no significant difference between S of 5-nitroxide stearate was observed for SHR and WKY rats, S of 10-nitroxide stearate was significantly lower in SHR than it was in WKY rat microsomal membrane, indicating that the core microsomal membrane fluidity was higher in SHR. Significant differences between fatty acid compositions were observed for SHR and WKY rat microsomal membranes. Delta9 and n-6 delta6 microsomal desaturase activities were significantly lower in SHR.

CONCLUSION

These results suggest that the higher liver core microsomal membrane fluidity observed in SHR might be dependent on the increased proportion of mono-unsaturated fatty acids. Such observed modifications and the alterations in delta9 and n-6 delta6 desaturase activities suggest that an impaired polyunsaturated fatty acid biosynthesis is related to changes in microsomal membrane fluidity in hypertension.

Authors+Show Affiliations

Unité de Nutrition Cellulaire et Métabolique, EA DGRT 1867, Université de Bourgogne, Faculté des Sciences, Dijon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9280209

Citation

Foucher, C, et al. "Liver Microsomal Membrane Fluidity and Microsomal Desaturase Activities in Adult Spontaneously Hypertensive Rats." Journal of Hypertension, vol. 15, no. 8, 1997, pp. 863-9.
Foucher C, Narce M, Nasr L, et al. Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats. J Hypertens. 1997;15(8):863-9.
Foucher, C., Narce, M., Nasr, L., Delachambre, M. C., & Poisson, J. P. (1997). Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats. Journal of Hypertension, 15(8), 863-9.
Foucher C, et al. Liver Microsomal Membrane Fluidity and Microsomal Desaturase Activities in Adult Spontaneously Hypertensive Rats. J Hypertens. 1997;15(8):863-9. PubMed PMID: 9280209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats. AU - Foucher,C, AU - Narce,M, AU - Nasr,L, AU - Delachambre,M C, AU - Poisson,J P, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 863 EP - 9 JF - Journal of hypertension JO - J Hypertens VL - 15 IS - 8 N2 - OBJECTIVE: The purpose of the present study was to investigate liver microsomal membrane fluidity simultaneously with membrane fatty acid composition and desaturase activities in spontaneously hypertensive rats (SHR). DESIGN AND METHODS: The membrane fluidity was determined, after electron spin resonance (ESR) measurement, in SHR compared with normotensive Wistar-Kyoto (WKY) rats, by calculating the order parameter S from ESR spectra of 5-nitroxide stearate and 10-nitroxide stearate, used as spin-labelled fatty acids. Desaturase activities were measured by incubating SHR and WKY rat liver microsomes with [14C]-radiolabeled fatty acids as substrates for desaturation reactions. The fatty acid composition of liver microsomal membranes was determined by gas-liquid chromatography. RESULTS: Whereas no significant difference between S of 5-nitroxide stearate was observed for SHR and WKY rats, S of 10-nitroxide stearate was significantly lower in SHR than it was in WKY rat microsomal membrane, indicating that the core microsomal membrane fluidity was higher in SHR. Significant differences between fatty acid compositions were observed for SHR and WKY rat microsomal membranes. Delta9 and n-6 delta6 microsomal desaturase activities were significantly lower in SHR. CONCLUSION: These results suggest that the higher liver core microsomal membrane fluidity observed in SHR might be dependent on the increased proportion of mono-unsaturated fatty acids. Such observed modifications and the alterations in delta9 and n-6 delta6 desaturase activities suggest that an impaired polyunsaturated fatty acid biosynthesis is related to changes in microsomal membrane fluidity in hypertension. SN - 0263-6352 UR - https://www.unboundmedicine.com/medline/citation/9280209/Liver_microsomal_membrane_fluidity_and_microsomal_desaturase_activities_in_adult_spontaneously_hypertensive_rats_ L2 - https://doi.org/10.1097/00004872-199715080-00010 DB - PRIME DP - Unbound Medicine ER -