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Relations between the extracellular concentrations of choline and acetylcholine in rat striatum.
J Neurochem. 1997 Sep; 69(3):1246-51.JN

Abstract

Changes in extracellular levels of acetylcholine (ACh) and choline (Ch) in the striatum of rats were examined by in vivo microdialysis after intraperitoneal injections of drugs. A dopamine D2 antagonist, sulpiride (20 mg/kg), and a muscarinic antagonist, atropine (3.5 mg/ kg), increased ACh levels and decreased Ch levels. On the contrary, the D2 agonist (+/-)-2-(N-phenylethyl-N-propyl)amino-5-hydroxytetralin (N-434; 5 mg/kg) and an anesthetic, pentobarbital (50 mg/kg), decreased ACh levels and increased Ch levels. Perfusion of 10 microM hemicholinium-3 (HC-3), a Ch uptake inhibitor, through the striatum induced a complete inhibition of ACh release and increased Ch levels in all drug-treated groups. The degree of relative increase in the level of Ch induced by HC-3 differed among the drug-pretreated groups; compared with the control group, the relative increase was larger in the sulpiride- and atropine-treated groups and smaller in the N-434 and pentobarbital-treated groups. Thus, we demonstrated reciprocal relations between extracellular concentrations of Ch and ACh after treatments by drugs. The data suggest that in the striatum, which is rich in cholinergic innervation, the extracellular Ch concentration is to a large extent determined by activity of the cholinergic transmission reflected in high-affinity choline uptake.

Authors+Show Affiliations

Department of Neuropsychopharmacology (Tsumura), Gunma University, School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9282949

Citation

Ikarashi, Y, et al. "Relations Between the Extracellular Concentrations of Choline and Acetylcholine in Rat Striatum." Journal of Neurochemistry, vol. 69, no. 3, 1997, pp. 1246-51.
Ikarashi Y, Takahashi A, Ishimaru H, et al. Relations between the extracellular concentrations of choline and acetylcholine in rat striatum. J Neurochem. 1997;69(3):1246-51.
Ikarashi, Y., Takahashi, A., Ishimaru, H., Arai, T., & Maruyama, Y. (1997). Relations between the extracellular concentrations of choline and acetylcholine in rat striatum. Journal of Neurochemistry, 69(3), 1246-51.
Ikarashi Y, et al. Relations Between the Extracellular Concentrations of Choline and Acetylcholine in Rat Striatum. J Neurochem. 1997;69(3):1246-51. PubMed PMID: 9282949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relations between the extracellular concentrations of choline and acetylcholine in rat striatum. AU - Ikarashi,Y, AU - Takahashi,A, AU - Ishimaru,H, AU - Arai,T, AU - Maruyama,Y, PY - 1997/9/1/pubmed PY - 1997/9/1/medline PY - 1997/9/1/entrez SP - 1246 EP - 51 JF - Journal of neurochemistry JO - J Neurochem VL - 69 IS - 3 N2 - Changes in extracellular levels of acetylcholine (ACh) and choline (Ch) in the striatum of rats were examined by in vivo microdialysis after intraperitoneal injections of drugs. A dopamine D2 antagonist, sulpiride (20 mg/kg), and a muscarinic antagonist, atropine (3.5 mg/ kg), increased ACh levels and decreased Ch levels. On the contrary, the D2 agonist (+/-)-2-(N-phenylethyl-N-propyl)amino-5-hydroxytetralin (N-434; 5 mg/kg) and an anesthetic, pentobarbital (50 mg/kg), decreased ACh levels and increased Ch levels. Perfusion of 10 microM hemicholinium-3 (HC-3), a Ch uptake inhibitor, through the striatum induced a complete inhibition of ACh release and increased Ch levels in all drug-treated groups. The degree of relative increase in the level of Ch induced by HC-3 differed among the drug-pretreated groups; compared with the control group, the relative increase was larger in the sulpiride- and atropine-treated groups and smaller in the N-434 and pentobarbital-treated groups. Thus, we demonstrated reciprocal relations between extracellular concentrations of Ch and ACh after treatments by drugs. The data suggest that in the striatum, which is rich in cholinergic innervation, the extracellular Ch concentration is to a large extent determined by activity of the cholinergic transmission reflected in high-affinity choline uptake. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/9282949/Relations_between_the_extracellular_concentrations_of_choline_and_acetylcholine_in_rat_striatum_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=1997&volume=69&issue=3&spage=1246 DB - PRIME DP - Unbound Medicine ER -