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Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries.
Br J Pharmacol. 1997 Aug; 121(8):1509-11.BJ

Abstract

The endogenous cannabinoid, anandamide, has been suggested as an endothelium-derived hyperpolarizing factor (EDHF). We found that anandamide-evoked relaxation in isolated segments of rat mesenteric artery was associated with smooth muscle hyperpolarization. However, although anandamide-evoked relaxation was inhibited by either charybdotoxin (ChTX) or iberiotoxin, inhibition of the relaxation to EDHF required a combination of ChTX and apamin. The relaxations induced by either anandamide or EDHF were not inhibited by the cannabinoid receptor (CB1) antagonist SRI41716A, or mimicked by selective CB1 agonists. Thus, anandamide appears to cause smooth muscle relaxation via a CB1 receptor-independent mechanism and cannabinoid receptor activation apparently does not contribute to EDHF-mediated relaxation in this resistance artery.

Authors+Show Affiliations

Department of Pharmacology, University of Bristol.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9283682

Citation

Plane, F, et al. "Evidence That Anandamide and EDHF Act Via Different Mechanisms in Rat Isolated Mesenteric Arteries." British Journal of Pharmacology, vol. 121, no. 8, 1997, pp. 1509-11.
Plane F, Holland M, Waldron GJ, et al. Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries. Br J Pharmacol. 1997;121(8):1509-11.
Plane, F., Holland, M., Waldron, G. J., Garland, C. J., & Boyle, J. P. (1997). Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries. British Journal of Pharmacology, 121(8), 1509-11.
Plane F, et al. Evidence That Anandamide and EDHF Act Via Different Mechanisms in Rat Isolated Mesenteric Arteries. Br J Pharmacol. 1997;121(8):1509-11. PubMed PMID: 9283682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries. AU - Plane,F, AU - Holland,M, AU - Waldron,G J, AU - Garland,C J, AU - Boyle,J P, PY - 1997/8/1/pubmed PY - 1997/8/1/medline PY - 1997/8/1/entrez SP - 1509 EP - 11 JF - British journal of pharmacology JO - Br J Pharmacol VL - 121 IS - 8 N2 - The endogenous cannabinoid, anandamide, has been suggested as an endothelium-derived hyperpolarizing factor (EDHF). We found that anandamide-evoked relaxation in isolated segments of rat mesenteric artery was associated with smooth muscle hyperpolarization. However, although anandamide-evoked relaxation was inhibited by either charybdotoxin (ChTX) or iberiotoxin, inhibition of the relaxation to EDHF required a combination of ChTX and apamin. The relaxations induced by either anandamide or EDHF were not inhibited by the cannabinoid receptor (CB1) antagonist SRI41716A, or mimicked by selective CB1 agonists. Thus, anandamide appears to cause smooth muscle relaxation via a CB1 receptor-independent mechanism and cannabinoid receptor activation apparently does not contribute to EDHF-mediated relaxation in this resistance artery. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/9283682/Evidence_that_anandamide_and_EDHF_act_via_different_mechanisms_in_rat_isolated_mesenteric_arteries_ L2 - https://doi.org/10.1038/sj.bjp.0701361 DB - PRIME DP - Unbound Medicine ER -