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Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts.
J Cell Physiol. 1997 Sep; 172(3):361-72.JC

Abstract

Corneal epithelial stem cells and transient amplifying cells are located in the limbal and corneal regions, respectively. In a serum-free medium with or without different cytokines, limbal fibroblasts consistently produced greater levels of keratinocyte growth factor (KGF) transcript and protein than corneal fibroblasts, whereas corneal fibroblasts produced greater levels of hepatocyte growth factor/ scatter factor (HGF/SF) transcript and protein than limbal fibroblasts. Expression of HGF/SF transcript and protein was up-regulated mildly by epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), or platelet-derived growth factor B (PDGF-BB) but markedly by interleukin-1 beta (IL-1 beta) and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF transcript was down-regulated by EGF, TGF-alpha, and PDGF-BB, was markedly up-regulated by IL-1 beta, and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF protein was up-regulated markedly by IL-1 beta and moderately by PDGF-BB, especially in limbal fibroblasts. TGF-beta 1 uniquely turned off transcript and protein expression of HGF/SF and KGF in corneal fibroblasts. Although its transcript levels were similarly down-regulated in limbal fibroblasts, KGF protein levels were paradoxically up-regulated by TGF-beta 1 when added alone or with TGF-alpha or IL-1 beta. These data indicate that KGF and HGF/SF, two fibroblast-derived epithelial mitogens, are expressed differentially by limbal and corneal fibroblasts and are modulated by cytokines activated during epithelial-mesenchymal interactions, suggesting that they may play a different role in modulating corneal epithelial stem cells and transient amplifying cells.

Authors+Show Affiliations

Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida 33101, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9284956

Citation

Li, D Q., and S C. Tseng. "Differential Regulation of Keratinocyte Growth Factor and Hepatocyte Growth Factor/scatter Factor By Different Cytokines in Human Corneal and Limbal Fibroblasts." Journal of Cellular Physiology, vol. 172, no. 3, 1997, pp. 361-72.
Li DQ, Tseng SC. Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts. J Cell Physiol. 1997;172(3):361-72.
Li, D. Q., & Tseng, S. C. (1997). Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts. Journal of Cellular Physiology, 172(3), 361-72.
Li DQ, Tseng SC. Differential Regulation of Keratinocyte Growth Factor and Hepatocyte Growth Factor/scatter Factor By Different Cytokines in Human Corneal and Limbal Fibroblasts. J Cell Physiol. 1997;172(3):361-72. PubMed PMID: 9284956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts. AU - Li,D Q, AU - Tseng,S C, PY - 1997/9/1/pubmed PY - 2000/6/20/medline PY - 1997/9/1/entrez SP - 361 EP - 72 JF - Journal of cellular physiology JO - J Cell Physiol VL - 172 IS - 3 N2 - Corneal epithelial stem cells and transient amplifying cells are located in the limbal and corneal regions, respectively. In a serum-free medium with or without different cytokines, limbal fibroblasts consistently produced greater levels of keratinocyte growth factor (KGF) transcript and protein than corneal fibroblasts, whereas corneal fibroblasts produced greater levels of hepatocyte growth factor/ scatter factor (HGF/SF) transcript and protein than limbal fibroblasts. Expression of HGF/SF transcript and protein was up-regulated mildly by epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), or platelet-derived growth factor B (PDGF-BB) but markedly by interleukin-1 beta (IL-1 beta) and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF transcript was down-regulated by EGF, TGF-alpha, and PDGF-BB, was markedly up-regulated by IL-1 beta, and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF protein was up-regulated markedly by IL-1 beta and moderately by PDGF-BB, especially in limbal fibroblasts. TGF-beta 1 uniquely turned off transcript and protein expression of HGF/SF and KGF in corneal fibroblasts. Although its transcript levels were similarly down-regulated in limbal fibroblasts, KGF protein levels were paradoxically up-regulated by TGF-beta 1 when added alone or with TGF-alpha or IL-1 beta. These data indicate that KGF and HGF/SF, two fibroblast-derived epithelial mitogens, are expressed differentially by limbal and corneal fibroblasts and are modulated by cytokines activated during epithelial-mesenchymal interactions, suggesting that they may play a different role in modulating corneal epithelial stem cells and transient amplifying cells. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/9284956/Differential_regulation_of_keratinocyte_growth_factor_and_hepatocyte_growth_factor/scatter_factor_by_different_cytokines_in_human_corneal_and_limbal_fibroblasts_ L2 - https://doi.org/10.1002/(SICI)1097-4652(199709)172:3<361::AID-JCP10>3.0.CO;2-9 DB - PRIME DP - Unbound Medicine ER -