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Characterization of cytokine profiles and double-positive lymphocyte subpopulations in normal bovine lungs.
Am J Vet Res. 1997 Sep; 58(9):969-75.AJ

Abstract

OBJECTIVE

To characterize cytokine profiles and lymphocyte subpopulations in lung parenchyma and bronchoalveolar lavage (BAL) fluid from normal bovine lungs.

ANIMALS

Eight 12- to 18-month-old cattle.

PROCEDURE

Cell populations in BAL fluid and collagenase-digested lung parenchyma were analyzed by flow cytometry and monoclonal antibodies. Proportions of total cell populations were determined, using Giemsa-stained cytospots. Distribution of lymphocytes within the lung parenchyma was analyzed by immunohistochemistry, and cytokine mRNA species in the parenchyma were characterized by use of reverse transcriptase-polymerase chain reaction analysis.

RESULTS

Cytokine profiles indicated high amounts of mRNA for interleukins 6 and 10 and transforming growth factor beta. In the BAL fluid and lung parenchyma, macrophages were the predominant cell type, although the proportion was lower in the parenchyma. Lymphocytes made up approximately 3% of both cell populations. Common to both lung compartments was the predominance of CD2+ and gamma delta T cells over B lymphocytes. There were more CD8+ T cells than CD4+ T cells in both compartments. The gamma delta cells made up approximately 9% of the lymphocyte populations. Two-color flow cytometry revealed CD8+ gamma delta T cell and CD8+CD5- populations that were unique to BAL fluid. In the BAL fluid and parenchyma, most CD4+ and CD8+ T cells expressed high amounts of CD44, a characteristic of memory T cells. The gamma delta T cells were CD44(10), as were B cells in the lung parenchyma. The B cells from BAL fluid expressed high amounts of CD44. Immunohistologic analysis of lung tissue revealed bronchus-associated lymphoid tissue structures with distinctive germinal center organization of B cells encompassed by CD4+ T cells.

CONCLUSIONS

Results provided normal values for comparison with those of other species and with the bovine respiratory tract response to disease.

Authors+Show Affiliations

Centre for Animal Biotechnology, University of Melbourne, Parkville, Victoria, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9285000

Citation

Mathy, N L., et al. "Characterization of Cytokine Profiles and Double-positive Lymphocyte Subpopulations in Normal Bovine Lungs." American Journal of Veterinary Research, vol. 58, no. 9, 1997, pp. 969-75.
Mathy NL, Walker J, Lee RP. Characterization of cytokine profiles and double-positive lymphocyte subpopulations in normal bovine lungs. Am J Vet Res. 1997;58(9):969-75.
Mathy, N. L., Walker, J., & Lee, R. P. (1997). Characterization of cytokine profiles and double-positive lymphocyte subpopulations in normal bovine lungs. American Journal of Veterinary Research, 58(9), 969-75.
Mathy NL, Walker J, Lee RP. Characterization of Cytokine Profiles and Double-positive Lymphocyte Subpopulations in Normal Bovine Lungs. Am J Vet Res. 1997;58(9):969-75. PubMed PMID: 9285000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of cytokine profiles and double-positive lymphocyte subpopulations in normal bovine lungs. AU - Mathy,N L, AU - Walker,J, AU - Lee,R P, PY - 1997/9/1/pubmed PY - 1997/9/1/medline PY - 1997/9/1/entrez SP - 969 EP - 75 JF - American journal of veterinary research JO - Am J Vet Res VL - 58 IS - 9 N2 - OBJECTIVE: To characterize cytokine profiles and lymphocyte subpopulations in lung parenchyma and bronchoalveolar lavage (BAL) fluid from normal bovine lungs. ANIMALS: Eight 12- to 18-month-old cattle. PROCEDURE: Cell populations in BAL fluid and collagenase-digested lung parenchyma were analyzed by flow cytometry and monoclonal antibodies. Proportions of total cell populations were determined, using Giemsa-stained cytospots. Distribution of lymphocytes within the lung parenchyma was analyzed by immunohistochemistry, and cytokine mRNA species in the parenchyma were characterized by use of reverse transcriptase-polymerase chain reaction analysis. RESULTS: Cytokine profiles indicated high amounts of mRNA for interleukins 6 and 10 and transforming growth factor beta. In the BAL fluid and lung parenchyma, macrophages were the predominant cell type, although the proportion was lower in the parenchyma. Lymphocytes made up approximately 3% of both cell populations. Common to both lung compartments was the predominance of CD2+ and gamma delta T cells over B lymphocytes. There were more CD8+ T cells than CD4+ T cells in both compartments. The gamma delta cells made up approximately 9% of the lymphocyte populations. Two-color flow cytometry revealed CD8+ gamma delta T cell and CD8+CD5- populations that were unique to BAL fluid. In the BAL fluid and parenchyma, most CD4+ and CD8+ T cells expressed high amounts of CD44, a characteristic of memory T cells. The gamma delta T cells were CD44(10), as were B cells in the lung parenchyma. The B cells from BAL fluid expressed high amounts of CD44. Immunohistologic analysis of lung tissue revealed bronchus-associated lymphoid tissue structures with distinctive germinal center organization of B cells encompassed by CD4+ T cells. CONCLUSIONS: Results provided normal values for comparison with those of other species and with the bovine respiratory tract response to disease. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/9285000/Characterization_of_cytokine_profiles_and_double_positive_lymphocyte_subpopulations_in_normal_bovine_lungs_ L2 - https://antibodies.cancer.gov/detail/CPTC-CD44-1 DB - PRIME DP - Unbound Medicine ER -