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Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1.
Genomics. 1997 Aug 15; 44(1):94-100.G

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant familial cancer syndrome characterized by parathyroid, pancreatic, and anterior pituitary tumors. The MEN1 locus has been previously localized to chromosome 11q13, and a 2-Mb gene-rich region flanked by D11S1883 and D11S449 has been defined. We have pursued studies to facilitate identification of the MEN1 gene by narrowing this critical region to a 900-kb interval between the VRF and D11S1783 loci through melotic mapping. This was achieved by investigating 17 cosmids for microsatellite polymorphisms, which defined two novel polymorphisms at the VRF and A0138 loci, and utilizing these to characterize recombinants in MEN1 families. In addition, we have established a 1200-kb sequence-ready contig consisting of 26 cosmids, eight BACs, and eight PACs that encompass this region. The precise locations for 19 genes and three ESTs within this contig have been determined, and three gene clusters consisting of a centromeric group (VRF, FKBP2, PNG, and PLCB3), a middle group (PYGM, ZFM1, SCG1, SCG2 (which proved to be the MEN1 gene), and PPP2R5B), and a telomeric group (H4B, ANG3, ANG2, ANG1, FON, FAU, NOF, NON, and D11S2196E) were observed. These results represent a valuable transcriptional map of chromosome 11q13 that will help in the search for disease genes in this region.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9286704

Citation

Lemmens, I, et al. "Construction of a 1.2-Mb Sequence-ready Contig of Chromosome 11q13 Encompassing the Multiple Endocrine Neoplasia Type 1 (MEN1) Gene. the European Consortium On MEN1." Genomics, vol. 44, no. 1, 1997, pp. 94-100.
Lemmens I, Merregaert J, Van de Ven WJ, et al. Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1. Genomics. 1997;44(1):94-100.
Lemmens, I., Merregaert, J., Van de Ven, W. J., Kas, K., Zhang, C. X., Giraud, S., Wautot, V., Buisson, N., De Witte, K., Salandre, J., Lenoir, G., Calender, A., Parente, F., Quincey, D., Courseaux, A., Carle, G. F., Gaudray, P., De Wit, M. J., Lips, C. J., ... Thakker, R. V. (1997). Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1. Genomics, 44(1), 94-100.
Lemmens I, et al. Construction of a 1.2-Mb Sequence-ready Contig of Chromosome 11q13 Encompassing the Multiple Endocrine Neoplasia Type 1 (MEN1) Gene. the European Consortium On MEN1. Genomics. 1997 Aug 15;44(1):94-100. PubMed PMID: 9286704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1. A1 - Lemmens,I, AU - Merregaert,J, AU - Van de Ven,W J, AU - Kas,K, AU - Zhang,C X, AU - Giraud,S, AU - Wautot,V, AU - Buisson,N, AU - De Witte,K, AU - Salandre,J, AU - Lenoir,G, AU - Calender,A, AU - Parente,F, AU - Quincey,D, AU - Courseaux,A, AU - Carle,G F, AU - Gaudray,P, AU - De Wit,M J, AU - Lips,C J, AU - Höppener,J W, AU - Khodaei,S, AU - Grant,A L, AU - Weber,G, AU - Kytölä,S, AU - Thakker,R V, PY - 1997/8/15/pubmed PY - 1997/8/15/medline PY - 1997/8/15/entrez SP - 94 EP - 100 JF - Genomics JO - Genomics VL - 44 IS - 1 N2 - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant familial cancer syndrome characterized by parathyroid, pancreatic, and anterior pituitary tumors. The MEN1 locus has been previously localized to chromosome 11q13, and a 2-Mb gene-rich region flanked by D11S1883 and D11S449 has been defined. We have pursued studies to facilitate identification of the MEN1 gene by narrowing this critical region to a 900-kb interval between the VRF and D11S1783 loci through melotic mapping. This was achieved by investigating 17 cosmids for microsatellite polymorphisms, which defined two novel polymorphisms at the VRF and A0138 loci, and utilizing these to characterize recombinants in MEN1 families. In addition, we have established a 1200-kb sequence-ready contig consisting of 26 cosmids, eight BACs, and eight PACs that encompass this region. The precise locations for 19 genes and three ESTs within this contig have been determined, and three gene clusters consisting of a centromeric group (VRF, FKBP2, PNG, and PLCB3), a middle group (PYGM, ZFM1, SCG1, SCG2 (which proved to be the MEN1 gene), and PPP2R5B), and a telomeric group (H4B, ANG3, ANG2, ANG1, FON, FAU, NOF, NON, and D11S2196E) were observed. These results represent a valuable transcriptional map of chromosome 11q13 that will help in the search for disease genes in this region. SN - 0888-7543 UR - https://www.unboundmedicine.com/medline/citation/9286704/Construction_of_a_1_2_Mb_sequence_ready_contig_of_chromosome_11q13_encompassing_the_multiple_endocrine_neoplasia_type_1__MEN1__gene__The_European_Consortium_on_MEN1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0888754397948722 DB - PRIME DP - Unbound Medicine ER -