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In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype.
Blood. 1997 Sep 01; 90(5):2089-96.Blood

Abstract

We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phenotype could be generated in vitro, and that such functionally defined T-cell subsets mediated a graft-versus-leukemia (GVL) effect with reduced graft-versus-host disease (GVHD). To evaluate whether analogous Tc1 and Tc2 subsets might be generated in humans, CD8+ T cells were allostimulated in the presence of either interleukin-12 (IL-12) and transforming growth factor-beta (TGF-beta) (Tc1 culture) or IL-4 (Tc2 culture). Tc1-type CD8 cells secreted the type I cytokines IL-2 and interferon gamma (IFN-gamma), whereas Tc2-type cells primarily secreted the type II cytokines IL-4, IL-5, and IL-10. Both cytokine-secreting populations effectively lysed tumor targets when stimulated with anti-T-cell receptor (TCR) antibody; allospecificity of Tc1- and Tc2-mediated cytolytic function was demonstrated using bone marrow-derived stimulator cells as targets. In addition, both Tc1 and Tc2 subsets were capable of mediating cytolysis through the fas pathway. We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype can be generated in vitro, and that these T-cell populations may be important for the mediation and regulation of allogeneic transplantation responses.

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Authors+Show Affiliations

Halverson DC
Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Schwartz GN
No affiliation info available
Carter C
No affiliation info available
Gress RE
No affiliation info available
Fowler DH
No affiliation info available

MeSH

AnimalsCD8 AntigensCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCytokinesCytotoxicity, ImmunologicHumansIsoantigensLymphocyte ActivationMiceT-Lymphocyte Subsets

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9292548

Citation

Halverson, D C., et al. "In Vitro Generation of Allospecific Human CD8+ T Cells of Tc1 and Tc2 Phenotype." Blood, vol. 90, no. 5, 1997, pp. 2089-96.
Halverson DC, Schwartz GN, Carter C, et al. In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. Blood. 1997;90(5):2089-96.
Halverson, D. C., Schwartz, G. N., Carter, C., Gress, R. E., & Fowler, D. H. (1997). In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. Blood, 90(5), 2089-96.
Halverson DC, et al. In Vitro Generation of Allospecific Human CD8+ T Cells of Tc1 and Tc2 Phenotype. Blood. 1997 Sep 1;90(5):2089-96. PubMed PMID: 9292548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. AU - Halverson,D C, AU - Schwartz,G N, AU - Carter,C, AU - Gress,R E, AU - Fowler,D H, PY - 1997/9/18/pubmed PY - 1997/9/18/medline PY - 1997/9/18/entrez SP - 2089 EP - 96 JF - Blood JO - Blood VL - 90 IS - 5 N2 - We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phenotype could be generated in vitro, and that such functionally defined T-cell subsets mediated a graft-versus-leukemia (GVL) effect with reduced graft-versus-host disease (GVHD). To evaluate whether analogous Tc1 and Tc2 subsets might be generated in humans, CD8+ T cells were allostimulated in the presence of either interleukin-12 (IL-12) and transforming growth factor-beta (TGF-beta) (Tc1 culture) or IL-4 (Tc2 culture). Tc1-type CD8 cells secreted the type I cytokines IL-2 and interferon gamma (IFN-gamma), whereas Tc2-type cells primarily secreted the type II cytokines IL-4, IL-5, and IL-10. Both cytokine-secreting populations effectively lysed tumor targets when stimulated with anti-T-cell receptor (TCR) antibody; allospecificity of Tc1- and Tc2-mediated cytolytic function was demonstrated using bone marrow-derived stimulator cells as targets. In addition, both Tc1 and Tc2 subsets were capable of mediating cytolysis through the fas pathway. We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype can be generated in vitro, and that these T-cell populations may be important for the mediation and regulation of allogeneic transplantation responses. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/9292548/In_vitro_generation_of_allospecific_human_CD8+_T_cells_of_Tc1_and_Tc2_phenotype_ L2 - https://ashpublications.org/blood/article-lookup/doi/&lo.doi; DB - PRIME DP - Unbound Medicine ER -