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In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype.
Blood. 1997 Sep 01; 90(5):2089-96.Blood

Abstract

We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phenotype could be generated in vitro, and that such functionally defined T-cell subsets mediated a graft-versus-leukemia (GVL) effect with reduced graft-versus-host disease (GVHD). To evaluate whether analogous Tc1 and Tc2 subsets might be generated in humans, CD8+ T cells were allostimulated in the presence of either interleukin-12 (IL-12) and transforming growth factor-beta (TGF-beta) (Tc1 culture) or IL-4 (Tc2 culture). Tc1-type CD8 cells secreted the type I cytokines IL-2 and interferon gamma (IFN-gamma), whereas Tc2-type cells primarily secreted the type II cytokines IL-4, IL-5, and IL-10. Both cytokine-secreting populations effectively lysed tumor targets when stimulated with anti-T-cell receptor (TCR) antibody; allospecificity of Tc1- and Tc2-mediated cytolytic function was demonstrated using bone marrow-derived stimulator cells as targets. In addition, both Tc1 and Tc2 subsets were capable of mediating cytolysis through the fas pathway. We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype can be generated in vitro, and that these T-cell populations may be important for the mediation and regulation of allogeneic transplantation responses.

Authors+Show Affiliations

Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9292548

Citation

Halverson, D C., et al. "In Vitro Generation of Allospecific Human CD8+ T Cells of Tc1 and Tc2 Phenotype." Blood, vol. 90, no. 5, 1997, pp. 2089-96.
Halverson DC, Schwartz GN, Carter C, et al. In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. Blood. 1997;90(5):2089-96.
Halverson, D. C., Schwartz, G. N., Carter, C., Gress, R. E., & Fowler, D. H. (1997). In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. Blood, 90(5), 2089-96.
Halverson DC, et al. In Vitro Generation of Allospecific Human CD8+ T Cells of Tc1 and Tc2 Phenotype. Blood. 1997 Sep 1;90(5):2089-96. PubMed PMID: 9292548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro generation of allospecific human CD8+ T cells of Tc1 and Tc2 phenotype. AU - Halverson,D C, AU - Schwartz,G N, AU - Carter,C, AU - Gress,R E, AU - Fowler,D H, PY - 1997/9/18/pubmed PY - 1997/9/18/medline PY - 1997/9/18/entrez SP - 2089 EP - 96 JF - Blood JO - Blood VL - 90 IS - 5 N2 - We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phenotype could be generated in vitro, and that such functionally defined T-cell subsets mediated a graft-versus-leukemia (GVL) effect with reduced graft-versus-host disease (GVHD). To evaluate whether analogous Tc1 and Tc2 subsets might be generated in humans, CD8+ T cells were allostimulated in the presence of either interleukin-12 (IL-12) and transforming growth factor-beta (TGF-beta) (Tc1 culture) or IL-4 (Tc2 culture). Tc1-type CD8 cells secreted the type I cytokines IL-2 and interferon gamma (IFN-gamma), whereas Tc2-type cells primarily secreted the type II cytokines IL-4, IL-5, and IL-10. Both cytokine-secreting populations effectively lysed tumor targets when stimulated with anti-T-cell receptor (TCR) antibody; allospecificity of Tc1- and Tc2-mediated cytolytic function was demonstrated using bone marrow-derived stimulator cells as targets. In addition, both Tc1 and Tc2 subsets were capable of mediating cytolysis through the fas pathway. We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype can be generated in vitro, and that these T-cell populations may be important for the mediation and regulation of allogeneic transplantation responses. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/9292548/In_vitro_generation_of_allospecific_human_CD8+_T_cells_of_Tc1_and_Tc2_phenotype_ L2 - https://ashpublications.org/blood/article-lookup/doi/&lo.doi; DB - PRIME DP - Unbound Medicine ER -